Ultra-tough injectable cytocompatible hydrogel for 3D cell culture and cartilage repair

Zhao, Yanran; Li, Mengnan; Liu, Bingchuan; Xiang, Junfeng; Cui, Zhiyong; Qu, Xiaozhong; Qiu, Dong; Yang, Zhenzhong

doi:10.1039/c7tb03177g

Cited by 51 publications

(53 citation statements)

References 51 publications

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“…A,B) Plots of the compressive versus tensile strength and modulus for BC–PVA–PAMPS (this work) and other strong hydrogels (see Table S1 in the Supporting Information for data and references). [ 13–35 ] The multiple data points for BC–PVA–PAMPS are for different compositions. C) BC–PVA–PAMPS easily bears the weight of a 100 lb.…”

Section: Introductionmentioning

confidence: 99%

A Synthetic Hydrogel Composite with the Mechanical Behavior and Durability of Cartilage

Yang

Zhao

Koshut

et al. 2020

Adv Funct Materials

204

178

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This article reports the first hydrogel with the strength and modulus of cartilage in both tension and compression, and the first to exhibit cartilage‐equivalent tensile fatigue strength at 100 000 cycles. These properties are achieved by infiltrating a bacterial cellulose (BC) nanofiber network with a poly(vinyl alcohol) (PVA)–poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid sodium salt) (PAMPS) double network hydrogel. The BC provides tensile strength in a manner analogous to collagen in cartilage, while the PAMPS provides a fixed negative charge and osmotic restoring force similar to the role of aggrecan in cartilage. The hydrogel has the same aggregate modulus and permeability as cartilage, resulting in the same time‐dependent deformation under confined compression. The hydrogel is not cytotoxic, has a coefficient of friction 45% lower than cartilage, and is 4.4 times more wear‐resistant than a PVA hydrogel. The properties of this hydrogel make it an excellent candidate material for replacement of damaged cartilage.

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Section: Introductionmentioning

confidence: 99%

A Synthetic Hydrogel Composite with the Mechanical Behavior and Durability of Cartilage

Yang

Zhao

Koshut

et al. 2020

Adv Funct Materials

204

178

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“…As depicted in Figure 1, two kinds of water-soluble naturally derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA), were chosen to fabricate injectable hydrogels. The oxidized alginate (OSA) with aldehyde groups was obtained via the periodate oxidation reaction, modified on our previous work [30]. The oxidation reaction was confirmed by 1 H-NMR spectrum of SA and OSA ( Figure S1).…”

Section: Design and Construction Of Cmc-osa-dtp Hydrogelsmentioning

confidence: 91%

A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds

et al. 2020

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Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance. Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials. The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications. In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels. Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3′-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds. Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability. In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro. Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.

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“…The dynamic gathering of CPBA could lead to a self-reinforcing effect inside the hydrogel matrix, resulting in high compressive and tensile moduli of the hydrogel over 1.0 MPa, including the highest compressive modulus up to 5.6 MPa. Moreover, after 3 months of implantation of this hydrogel into osteochondral defect in a rabbit model, smooth and complete cartilage layer was obtained, and the enrichment of glycosaminoglycan (GAG) and type-II collagen were observed in the cartilage layer (Zhao et al, 2018).…”

Section: Ionic Bonds Interactionsmentioning

confidence: 99%

“…Hydrogels crosslinked by ionic bonds interactions could also be sufficiently robust for cartilage regeneration, which was examined on rabbit model bearing articular osteochondral defect. Through the generation of ionic interaction and borate bonding, poly(vinyl alcohol) (PVA) was crosslinked by 4carboxyphenylboronic acid (CPBA) to bridge the polymer chains with the presence of calcium ions (Zhao et al, 2018). The dynamic gathering of CPBA could lead to a self-reinforcing effect inside the hydrogel matrix, resulting in high compressive and tensile moduli of the hydrogel over 1.0 MPa, including the highest compressive modulus up to 5.6 MPa.…”

Section: Ionic Bonds Interactionsmentioning

confidence: 99%

Recent Progress in 3D Printing of Elastic and High-Strength Hydrogels for the Treatment of Osteochondral and Cartilage Diseases

Dai

Sun

Leng

et al. 2020

Front. Bioeng. Biotechnol.

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Despite considerable progress for the regenerative medicine, repair of full-thickness articular cartilage defects and osteochondral interface remains challenging. This low efficiency is largely due to the difficulties in recapitulating the stratified zonal architecture of articular cartilage and engineering complex gradients for bone-soft tissue interface. This has led to increased interest in three-dimensional (3D) printing technologies in the field of musculoskeletal tissue engineering. Printable and biocompatible hydrogels are attractive materials for 3D printing applications because they not only own high tunability and complexity, but also offer favorable biomimetic environments for live cells, such as porous structure, high water content, and bioactive molecule incorporation. However, conventional hydrogels are usually mechanically weak and brittle, which cannot reach the mechanical requirements for repair of articular cartilage defects and osteochondral interface. Therefore, the development of elastic and high-strength hydrogels for 3D printing in the repairment of cartilage defects and osteochondral interface is crucial. In this review, we summarized the recent progress in elastic and high-strength hydrogels for 3D printing and categorized them into six groups, namely ion bonds interactions, nanocomposites integrated in hydrogels, supramolecular guest–host interactions, hydrogen bonds interactions, dynamic covalent bonds interactions, and hydrophobic interactions. These 3D printed elastic and high-strength hydrogels may provide new insights for the treatment of osteochondral and cartilage diseases.

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Ultra-tough injectable cytocompatible hydrogel for 3D cell culture and cartilage repair

Abstract: A high-performance hydrogel was synthesized by a facile dual dynamic crosslinking strategy that showed injectability, cytocompatibility, broadly tunable mechanical properties and the potential for repair of load-bearing tissues.

Cited by 51 publications

References 51 publications

A Synthetic Hydrogel Composite with the Mechanical Behavior and Durability of Cartilage

A Synthetic Hydrogel Composite with the Mechanical Behavior and Durability of Cartilage

A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds

Recent Progress in 3D Printing of Elastic and High-Strength Hydrogels for the Treatment of Osteochondral and Cartilage Diseases

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