Ultra structure analysis of cell–cell interactions between pericytes and neutrophils in vitro

Pieper, Christian; Galla, Hans‐Joachim

doi:10.1016/j.bbrc.2014.01.159

Cited by 8 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resistance created by these final two layers depends on the tissue and their coverage. Pericytes have multiple roles in assisting transmigrating leukocytes during inflammation, including secretion of cytokines, remodeling and degradation of the basement membrane, and upregulation of adhesion molecules, such as ICAM-1, to guide migrating immune cells after they cross the EC barrier (187)(188)(189)(190). Expression of LAMs, at least in some part, appears to be tissue-dependent (Figure 1, Table 3) (186).…”

Section: Pericytes Backgroundmentioning

confidence: 99%

Sourcing cells for in vitro models of human vascular barriers of inflammation

McCloskey

Zhang²,

Ahmad³

et al. 2022

Front. Med. Technol.

View full text Add to dashboard Cite

The vascular system plays a critical role in the progression and resolution of inflammation. The contributions of the vascular endothelium to these processes, however, vary with tissue and disease state. Recently, tissue chip models have emerged as promising tools to understand human disease and for the development of personalized medicine approaches. Inclusion of a vascular component within these platforms is critical for properly evaluating most diseases, but many models to date use “generic” endothelial cells, which can preclude the identification of biomedically meaningful pathways and mechanisms. As the knowledge of vascular heterogeneity and immune cell trafficking throughout the body advances, tissue chip models should also advance to incorporate tissue-specific cells where possible. Here, we discuss the known heterogeneity of leukocyte trafficking in vascular beds of some commonly modeled tissues. We comment on the availability of different tissue-specific cell sources for endothelial cells and pericytes, with a focus on stem cell sources for the full realization of personalized medicine. We discuss sources available for the immune cells needed to model inflammatory processes and the findings of tissue chip models that have used the cells to studying transmigration.

show abstract

Section: Pericytes Backgroundmentioning

confidence: 99%

Sourcing cells for in vitro models of human vascular barriers of inflammation

McCloskey

Zhang²,

Ahmad³

et al. 2022

Front. Med. Technol.

View full text Add to dashboard Cite

show abstract

“…The ultrastructural details behind neutrophil and pericyte contacts were observed using scanning force microscopy [23]. TNF- α -stimulated porcine pericytes expressed ICAM-1 and interacted with neutrophils through cellular extensions that both cell types formed.…”

Section: Pericytes Mediate Leukocyte Diapedesismentioning

confidence: 99%

“…Pericyte extensions were generally broader and larger than neutrophil extensions. However, after neutrophils interacted with pericytes, neutrophil extensions grew to almost the same height and width as the pericyte extensions [23]. While Proebstl et al found that pericyte gap enlargement in inflammatory conditions was independent of interactions with neutrophils [30], another study reported that contact between neutrophils and pericytes causes gap enlargement in IL-1 β -stimulated cremasteric venules as well as pericyte relaxation in culture [27].…”

Section: Pericytes Mediate Leukocyte Diapedesismentioning

confidence: 99%

“…Pericytes are involved in fine-tuning inflammatory responses by controlling leukocyte diapedesis. Pericyte-mediated leukocyte diapedesis involves crawling through the basement membrane, adhesion to and crawling on pericytes, and finally migration through pericyte gaps into interstitial space towards inflammatory foci [23]. Before migrating through gaps between pericytes, neutrophils first interact with adhesion molecules on the surface of pericytes [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role and Molecular Mechanisms of Pericytes in Regulation of Leukocyte Diapedesis in Inflamed Tissues

Rudziak

Ellis

Kowalewska

2019

Mediators of Inflammation

View full text Add to dashboard Cite

Leukocyte recruitment is a hallmark of the inflammatory response. Migrating leukocytes breach the endothelium along with the vascular basement membrane and associated pericytes. While much is known about leukocyte-endothelial cell interactions, the mechanisms and role of pericytes in extravasation are poorly understood and the classical paradigm of leukocyte recruitment in the microvasculature seldom adequately discusses the involvement of pericytes. Emerging evidence shows that pericytes are essential players in the regulation of leukocyte extravasation in addition to their functions in blood vessel formation and blood-brain barrier maintenance. Junctions between venular endothelial cells are closely aligned with extracellular matrix protein low expression regions (LERs) in the basement membrane, which in turn are aligned with gaps between pericytes. This forms preferential paths for leukocyte extravasation. Breaching of the layer formed by pericytes and the basement membrane entails remodelling of LERs, leukocyte-pericyte adhesion, crawling of leukocytes on pericyte processes, and enlargement of gaps between pericytes to form channels for migrating leukocytes. Furthermore, inflamed arteriolar and capillary pericytes induce chemotactic migration of leukocytes that exit postcapillary venules, and through direct pericyte-leukocyte contact, they induce efficient interstitial migration to enhance the immunosurveillance capacity of leukocytes. Given their role as regulators of leukocyte extravasation, proper pericyte function is imperative in inflammatory disease contexts such as diabetic retinopathy and sepsis. This review summarizes research on the molecular mechanisms by which pericytes mediate leukocyte diapedesis in inflamed tissues.

show abstract

Pregnancy-induced Cardiovascular Pathologies: Importance of Structural Components and Lipids

Aleksenko

Quaye

2020

The American Journal of the Medical Sciences

View full text Add to dashboard Cite

Ultra structure analysis of cell–cell interactions between pericytes and neutrophils in vitro

Cited by 8 publications

References 19 publications

Sourcing cells for in vitro models of human vascular barriers of inflammation

Sourcing cells for in vitro models of human vascular barriers of inflammation

Role and Molecular Mechanisms of Pericytes in Regulation of Leukocyte Diapedesis in Inflamed Tissues

Pregnancy-induced Cardiovascular Pathologies: Importance of Structural Components and Lipids

Contact Info

Product

Resources

About