Ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging

Wang, Chaoguang; Xiao, Rui; Dong, Peitao; Wu, Xuezhong; Rong, Zhen; Lin, Xin; Tang, Jun; Wang, Shengqi

doi:10.1016/j.bios.2014.01.016

Cited by 18 publications

(6 citation statements)

References 16 publications

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“…Some groups tried to develop such detection methods by using luminol and the specific hybridization of DNA sequences. This method was validated by the successful identification of pathogens that associated with infectious diarrheal diseases, such as Vibrio cholerae, Shigella , and Salmonella [185]. Such method should be extended to other diseases by immobilizing corresponding probe associated with those diseases.…”

Section: Future Prospectivementioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

228

181

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Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields.

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Section: Future Prospectivementioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

228

181

View full text Add to dashboard Cite

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“…thermal cycles, and special storage for protein enzymes, which may be difficult for resource-limited sites. Therefore, enzyme-free amplification has attracted increased 4 attention, including bio-barcode-based detection [34][35][36] , or hybridization DNA circuits [37][38][39][40][41][42] . The bio-barcode amplification uses target molecules to connect nanoparticles that carries vast number of barcode DNAs.…”

Section: Introductionmentioning

confidence: 99%

“…Visual detections of nucleic acids provide simple and rapid assays with visualized readout 1 , and have been widely used for pathogen identification [2][3][4][5] or disease diagnosis [6][7][8] . Nanomaterials, such as gold nanoparticles (AuNPs) 9-14 , silver nanoparticles 15 and graphene oxide 16 , have been employed for the visual detections according to their unique optical properties, biocompatibility, conductivity, and high surface-to-volume ratio [17][18][19][20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

“…Visual detection of nucleic acids provides simple and rapid assays with visualized readout, and has been widely used for pathogen identification − or disease diagnosis. − Nanomaterials, such as gold nanoparticles (AuNPs), − silver nanoparticles, and graphene oxide, have been employed for visual detection according to their unique optical properties, biocompatibility, conductivity, and high surface-to-volume ratio. − For example, AuNPs were used for detection of target molecules by modifying capturing probes on the surface of AuNPs. ,,− As such, the presence of target molecules would cause the aggregation of AuNPs and induce a change of solution color from red to purple. Moreover, this scheme was recently developed into lateral flow strips and magnetophoretic assays that provide fast visual readout for detection of ssDNA/RNA oligonucleotides. − However, although promise was shown, these visual detections are subject to the limit of detection (LOD), which usually falls into nanomole per liter level because a large amount of target oligonucleotides is required to induce AuNP aggregation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme-Free Amplification by Nano Sticky Balls for Visual Detection of ssDNA/RNA Oligonucleotides

Chen

Chu

Yeung

et al. 2015

ACS Appl. Mater. Interfaces

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Visual detection of nucleic acids provides simple and rapid screening for infectious diseases or environmental pathogens. However, sensitivity is the current bottleneck, which may require enzymatic amplification for targets in low abundance and make them incompatible with detection at resource-limited sites. Here we report an enzyme-free amplification that provides a sensitive visual detection of ssDNA/RNA oligonucleotides on the basis of nano "sticky balls". When target oligonucleotides are present, magnetic microparticles (MMPs) and gold nanoparticles (AuNPs) were linked together, allowing the collection of AuNPs after magnetic attraction. Subsequently, the collected AuNPs, which carry many oligonucleotides, were used as the sticky balls to link a second pair of MMPs and polymer microparticles (PMPs). Thus, because the magnetic field can attract the MMPs as well as the linked PMPs to the sidewall, the reduction of suspended PMPs yields a change of light transmission visible by the naked eye. Our results demonstrate that the limit of detection is 10 amol for ssDNAs (228 fM in 45 μL) and 75 amol for ssRNAs (1.67 pM in 45 μL). This method is also compatible with the serum environment and detection of a microRNA, miR-155, derived from human breast cancer cells. With significantly improved sensitivity for visual detection, it provides great potential for point-of-care applications at resource-limited sites.

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“…Short, single-stranded nucleic acids often serve as biomarkers of disease and bioterrorism agents. Their detection has broad applications, such as in pathogen identification [1][2][3][4] and disease diagnosis. 5,6 Many platforms have been developed for detecting nucleic acid molecules with specific sequences, including polymerase chain reaction (PCR), [7][8][9][10][11][12][13][14] bio-barcode-based detection 15,16 and electrochemistry.…”

Section: Introductionmentioning

confidence: 99%

Visual detection of nucleic acids based on Mie scattering and the magnetophoretic effect

Zhao

Chen

et al. 2015

Analyst

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Visual detection of nucleic acid biomarkers is a simple and convenient approach to point-of-care applications. However, issues of sensitivity and the handling of complex bio-fluids have posed challenges. Here we report on a visual method detecting nucleic acids using Mie scattering of polystyrene microparticles and the magnetophoretic effect. Magnetic microparticles (MMPs) and polystyrene microparticles (PMPs) were surface-functionalised with oligonucleotide probes, which can hybridise with target oligonucleotides in juxtaposition and lead to the formation of MMPs-targets-PMPs sandwich structures. Using an externally applied magnetic field, the magnetophoretic effect attracts the sandwich structure to the sidewall, which reduces the suspended PMPs and leads to a change in the light transmission via the Mie scattering. Based on the high extinction coefficient of the Mie scattering (∼3 orders of magnitude greater than that of the commonly used gold nanoparticles), our results showed the limit of detection to be 4 pM using a UV-Vis spectrometer or 10 pM by direct visual inspection. Meanwhile, we also demonstrated that this method is compatible with multiplex assays and detection in complex bio-fluids, such as whole blood or a pool of nucleic acids, without purification in advance. With a simplified operation procedure, low instrumentation requirement, high sensitivity and compatibility with complex bio-fluids, this method provides an ideal solution for visual detection of nucleic acids in resource-limited settings.

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Ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging

Cited by 18 publications

References 16 publications

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Enzyme-Free Amplification by Nano Sticky Balls for Visual Detection of ssDNA/RNA Oligonucleotides

Visual detection of nucleic acids based on Mie scattering and the magnetophoretic effect

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