Ultra-sensitive electrochemical immunosensor using analyte peptidomimetics selected from phage display peptide libraries

Electrochemical immunosensor for Enterobacter sakazakii detection based on electrochemically reduced graphene oxide–gold nanoparticle/ionic liquid modified electrode

“…In incubated with E. sakazakii (10 9 cfu/mL), B.cereus (10 9 cfu/mL), S. aureus (10 9 cfu/mL ), Vp (10 9 cfu/mL ), E. coli (10 9 cfu/mL ), Salmonella (10 9 cfu/mL) and PBS M A N U S C R I P T AUT/AuNPs/CHIT/MWNTs/SiO 2 @THI (CV) 4.12×10 2 -4.12×10 5 2.50×10 2 [46] Agarose/AuNPs (CV) 10 3 to 10 9 7.37 ×104 [47] CHI-SH/Fc/C 60 /Au-SiO 2 /GOD/PtNCs (CV) 3.2×10 1 -3.2×10 6 15 [16] ERGO-AuNPs /ILs (CV) 10 3 to 10 9 1. Table 2 A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 30 Graphical abstract…”

“…Electrochemical immunosensor is based on the specific binding of antibody to its antigen, with an increased sensitivity, selectivity and a reduction in analysis time [6,7]. Electrochemical immunosensor has attracted much research interest because of its high sensitivity, fast response, low cost and uncomplicated instrumentation [8][9][10][11].…”

Virus Outbreaks in Chemical and Biological Sensors

“…The long thread-like phages bound to the analyte-antibody immunocomplex can be visualized, with an increased sensitivity due to a larger surface area, by using HRP-conjugated anti-phage antibodies. Using this technique, they successfully detected small molecules, such as molinate [59,61], phenoxybenzoic acid (PBA) [60,62,63], brominated diphenyl ether 47 (BDE 47) [64], and clomazone [65]. Notably, the PHAIA method works well with a magnetic microbead-based immunoassay [62], PCR [63], and magneto-electrochemical immunoassay [61] (Figure 6).…”

“…Using this technique, they successfully detected small molecules, such as molinate [59,61], phenoxybenzoic acid (PBA) [60,62,63], brominated diphenyl ether 47 (BDE 47) [64], and clomazone [65]. Notably, the PHAIA method works well with a magnetic microbead-based immunoassay [62], PCR [63], and magneto-electrochemical immunoassay [61] (Figure 6). Similarly, a landscape phage developed by Petrenko et al in 1996 [39], carrying an analyte binding motif on all copies of the major coat protein pVIII, can replace primary antibodies with an exceptional multivalency in a sandwich immunoassay [66].…”

“…( a ) The increased surface area due to filamentous phages enhances detection signals using enzyme [61] (Copyright © 2011 Elsevier); ( b ) Analyte-bound phages can be amplified and identified by real-time PCR [63] (Copyright © 2010 ACS).…”