Ultra-sensitive electrochemical aptasensor for label-free detection of Aflatoxin B1 in wheat flour sample using factorial design experiments

Rahimi, Faezeh; Roshanfekr, Hamideh; Peyman, Hossein

doi:10.1016/j.foodchem.2020.128436

Cited by 45 publications

(27 citation statements)

References 29 publications

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“…In this study, electrochemical measurements were based on EIS and DPV. The AFB 1 dynamic range of 3 ag ml −1 −1.9 µg ml −1 and a low LOD of 0.9 ± 0.04 ag ml −1 were detected (Figure 7A) [36].…”

Section: Electrochemical Aptasensorsmentioning

confidence: 97%

“…The plentiful presence of carboxyl groups in the CQDs provided strong bonding of aptamers on the surface of CQDs with π-π interactions [35]. Rahimi and colleagues developed an ultrasensitive aptasensor based on an AFB 1 aptamer immobilized on CQD/octahedral Cu 2 O nanocomposites [36].…”

Section: Aptamer-functionalized Cnmsmentioning

confidence: 99%

“…CQD-Cu 2 O nanocomposites were used to improve the immobilization efficiency of the aptamer, thereby improving the electron transfer and increasing the sensitivity of the aptasensor [56]. Therefore, combined with the good electrochemical performance of octahedral Cu 2 O and the water solubility and biocompatibility of CQDs, an electrochemical aptasensor based on a Cu 2 O-CQD nanocomposite for the detection of AFB1 in a wheat flour sample was constructed [36]. ZnO and NGQDs were constructed by a one-step in situ conjugate method.…”

Section: Cnms Support Metal Oxide Nanoparticlesmentioning

confidence: 99%

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Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Xiang

Zhang

et al. 2021

Nanomaterials

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Carbon-based nanomaterials have become the subject of intensive interest because their intriguing physical and chemical properties are different from those of their bulk counterparts, leading to novel applications in smart sensors. Mycotoxins are secondary metabolites with different structures and toxic effects produced by fungi. Mycotoxins have low molecular weights and highly diverse molecular structures, which can induce a spectrum of biological effects in humans and animals even at low concentrations. A tremendous amount of biosensor platforms based on various carbon nanocomposites have been developed for the determination of mycotoxins. Therefore, the contents of this review are based on a balanced combination of our own studies and selected research studies performed by academic groups worldwide. We first address the vital preparation methods of biorecognition unit (antibodies, aptamers, molecularly imprinted polymers)-functionalized carbon-based nanomaterials for sensing mycotoxins. Then, we summarize various types of smart sensors for the detection of mycotoxins. We expect future research on smart sensors to show a significant impact on the detection of mycotoxins in food products.

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Section: Electrochemical Aptasensorsmentioning

confidence: 97%

Section: Aptamer-functionalized Cnmsmentioning

confidence: 99%

Section: Cnms Support Metal Oxide Nanoparticlesmentioning

confidence: 99%

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Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Xiang

Zhang

et al. 2021

Nanomaterials

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“…Therefore, a variety of GEC methods with excellent detection performance for detecting mycotoxins have been proposed [42][43][44]. Based on the detection signals, GEC biosensors for mycotoxins are described as below:…”

Section: General Electrochemical Biosensors For Mycotoxins Detectionmentioning

confidence: 99%

“…The erGO is not only used as modifying agent of electrode material with highly conductive, it can also be used to attach anti-HT2 (10) Fab by carbodiimide coupling. The composite GEC active material was prepared by mixing carbon quantum dots and octahedral Cu 2 O for DPV detecting AFB1 (Figure 1D) [43]. This composite material combined the excellent GEC activity of octahedral Cu 2 O and the biocompatibility and solubility of carbon quantum dots.…”

Section: Differential Pulse Voltammetry Biosensorsmentioning

confidence: 99%

Mycotoxins Detection Based on Electrochemical Approaches

et al. 2021

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Electrochemical biosensors have attracted much attention in mycotoxin bioanalysis. In this review, three electrochemical biosensor technologies for mycotoxins were reviewed, including general electrochemistry, photoelectrochemistry, and electrochemiluminescence. Based on the classification of multiple electrochemical detection methods, the design schemes, recognition mechanism and probe materials were described in detail. Moreover, the characteristics and limitations of these electrochemical biosensors were summarized. The challenges and future trends of electrochemical biosensor development in mycotoxin bioanalysis were also briefly discussed in the end. This review is expected to provide some inspirations for point-of-care testing in electrochemical sensors for mycotoxins and further electrochemical analysis application.

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Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†

et al. 2022

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Comprehensive Summary Ratiometric biosensor is among the promising strategies for the advanced analysis; its applicability in multiple scenarios, however, is seriously limited for the fixed analytical feature. Here, we reported ratiometric electrochemical biosensors with programmable analytical feature by alternating the binding site of ferrocene (Fc) to DNA duplex for the detection of aflatoxin B1 (AFB1). DNA duplex was synthesized with AFB1 aptamer and its complementary DNA (cDNA) with Fc molecular tagged on aptamer or cDNA. For analysis, aptamer‐AFB1 complex stripped from the electrode, and free cDNA triggered hybridization chain reaction to produce double‐stranded DNA for methylene blue (MB) absorption. In this way, redox currents of Fc (IFc) and absorbed MB (IMB) at the electrode were collected to produce the ratiometric signal of IMB/IFc as a yardstick for AFB1. When Fc was tagged on aptamer, IFc decreased with higher concentration of AFB1 (cAFB1), the resultant biosensor allowed detection with an On/Off (IMB/IFc) mode; when Fc was tagged on cDNA, IFc kept constant as cAFB1 varying, offering an On/Constant (IMB/IFc) mode for the detection. Consequently, biosensor with On/Off mode showed a linear range of 0.1—100 pg/mL with a limit of detection (LOD) of 0.0650 pg/mL, while biosensor with On/Constant mode offered a wider linear rang of 1—10000 pg/mL but a relatively poor LOD of 0.330 pg/mL. Our work revealed the vital importance of binding site of probes to DNA, and this principle can endow ratiometric biosensor with programmable analytical feature (e.g., linear range, LOD), allowing detections in various scenarios.

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Ultra-sensitive electrochemical aptasensor for label-free detection of Aflatoxin B1 in wheat flour sample using factorial design experiments

Cited by 45 publications

References 29 publications

Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Mycotoxins Detection Based on Electrochemical Approaches

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†

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Ultra-sensitive electrochemical aptasensor for label-free detection of Aflatoxin B1 in wheat flour sample using factorial design experiments

Cited by 45 publications

References 29 publications

Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins

Mycotoxins Detection Based on Electrochemical Approaches

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1†

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Product

Resources

About

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1^†