Ultra-sensitive DNAzyme-based optofluidic biosensor with liquid crystal-Au nanoparticle hybrid amplification for molecular detection

Wang, Ziyihui; Liu, Yize; Wang, Haonan; Wang, Shuang; Liu, Kun; Xu, Tianhua; Jiang, Junfeng; Chen, Yu‐Cheng

doi:10.1016/j.snb.2022.131608

Cited by 29 publications

(12 citation statements)

References 35 publications

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“…Wang et al developed a DNAzyme-based biosensor employing an LC–AuNPs hybrid amplified optofluidic resonator to realize the biodetection [ 134 ]. The microbubble resonator, with an outer diameter of 190

m and a wall thickness of 4

m, was obtained through the capillary stretching and expanding.…”

Section: Lc-based Whispering Gallery Mode Microcavity Biosensingmentioning

confidence: 99%

“… ( a ) Diagram of the DNAzyme optofluidic biosensor for L-histidine detection and the spectral response of the LC–AuNPs biosensor at different concentrations of L-histidine [ 134 ]; reproduced with permission from Elsevier. ( b ) Schematic of the optofluidic LC biosensor for protein assay detection [ 135 ]; reproduced with permission from Springer.…”

Section: Figurementioning

confidence: 99%

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Liquid Crystal Biosensors: Principles, Structure and Applications

Wang

et al. 2022

Biosensors

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Liquid crystals (LCs) have been widely used as sensitive elements to construct LC biosensors based on the principle that specific bonding events between biomolecules can affect the orientation of LC molecules. On the basis of the sensing interface of LC molecules, LC biosensors can be classified into three types: LC–solid interface sensing platforms, LC–aqueous interface sensing platforms, and LC–droplet interface sensing platforms. In addition, as a signal amplification method, the combination of LCs and whispering gallery mode (WGM) optical microcavities can provide higher detection sensitivity due to the extremely high quality factor and the small mode volume of the WGM optical microcavity, which enhances the interaction between the light field and biotargets. In this review, we present an overview of the basic principles, the structure, and the applications of LC biosensors. We discuss the important properties of LC and the principle of LC biosensors. The different geometries of LCs in the biosensing systems as well as their applications in the biological detection are then described. The fabrication and the application of the LC-based WGM microcavity optofluidic sensor in the biological detection are also introduced. Finally, challenges and potential research opportunities in the development of LC-based biosensors are discussed.

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m and a wall thickness of 4

m, was obtained through the capillary stretching and expanding.…”

Section: Lc-based Whispering Gallery Mode Microcavity Biosensingmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Liquid Crystal Biosensors: Principles, Structure and Applications

Wang

et al. 2022

Biosensors

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“…The reaction process is challenging to quantify and accurately characterize in these sensing modalities, limiting the further development of the LC sensing device in real-time monitoring. In order to achieve real-time quantitative monitoring of the analyte reaction process and to overcome these limitations [ 37 , 38 , 39 ], many groups use whispering gallery mode (WGM) lasers in LC droplets [ 40 , 41 , 42 ]. When light is incident from a light-dense medium to a light-sparse medium and the angle of incidence is large enough, total reflection can happen at the intersection of the two media, producing an optical WGM at the surface of the high-refractive index medium.…”

Section: Introductionmentioning

confidence: 99%

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Xie

et al. 2022

Biosensors

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The development of biosensing platforms has been impressively accelerated by advancements in liquid crystal (LC) technology. High response rate, easy operation, and good stability of the LC droplet-based biosensors are all benefits of the long-range order of LC molecules. Bioprobes emerged when LC droplets were combined with biotechnology, and these bioprobes are used extensively for disease diagnosis, food safety, and environmental monitoring. The LC droplet biosensors have high sensitivity and excellent selectivity, making them an attractive tool for the label-free, economical, and real-time detection of different targets. Portable devices work well as the accessory kits for LC droplet-based biosensors to make them easier to use by anyone for on-site monitoring of targets. Herein, we offer a review of the latest developments in the design of LC droplet-based biosensors for qualitative target monitoring and quantitative target analysis.

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“…Noble-metal nanoparticles (NPs) have been widely used in various fields, such as catalysis [ 1 , 2 ], biosensors [ 3 , 4 ], antibacterial materials [ 5 , 6 , 7 ] and pharmaceuticals [ 8 , 9 ], due to their unique physical and chemical properties. Considering its wide application in biomedical and clinical practice, the green synthesis of NPs by adopting reducing sugars as reductants and stabilizers has attracted the attention of many [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Xylan-Click-Quaternized Chitosan via Click Chemistry and Its Application in the Preparation of Nanometal Materials

et al. 2022

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For the high-valued utilization of hemicelluloses and for realizing the controllable synthesis of NPs, this paper’s aim is to combine xylan, chitosan and nanometal materials at the same time. In this research study, firstly, propargyl xylan was synthesized via nucleophilic substitution reaction between xylan and propargyl bromide in NaOH solution. On the other hand, a tosyl group was introduced onto the 6th position of synthesized quaternized chitosan (QCS), and the azide group replaced the tosyl group to obtain 6-amido-QCS (QCS-N3). The synthesis conditions of the above reactions were optimized. Subsequently, the novel xylan-click-QCS polymer was obtained via click reaction between terminal alkyne groups on the xylan chains and azide groups on QCS. Then, AgNPs and AuNPs were synthesized by adopting the xylan-click-QCS polymer as the reducing and stabilizing agent, and the reaction conditions were optimized to obtain well-dispersed and highly stable nanoparticles. There were two kinds of Ag nanomaterials, with diameters of 10~20 nm and 2~5 nm, respectively, indicating the formation of Ag nanoclusters, except for Ag nanoparticles, in this reaction. The diameter of the synthesized AuNPs was 20~30 nm, which possessed a more uniform size distribution. The Ag nanoclusters with a smaller size (2~5 nm) could inhibit MCF-7 cell proliferation effectively, indicating their application potential in cancer therapy. The study gives a new approach to the high-value utilization of biopolymers.

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Ultra-sensitive DNAzyme-based optofluidic biosensor with liquid crystal-Au nanoparticle hybrid amplification for molecular detection

Cited by 29 publications

References 35 publications

Liquid Crystal Biosensors: Principles, Structure and Applications

Liquid Crystal Biosensors: Principles, Structure and Applications

Liquid Crystal Droplet-Based Biosensors: Promising for Point-of-Care Testing

Synthesis of Xylan-Click-Quaternized Chitosan via Click Chemistry and Its Application in the Preparation of Nanometal Materials

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