Ultra-sensitive aptasensor based on a GQD nanocomposite for detection of hepatitis C virus core antigen

Ghanbari, Kazhal; Roushani, Mahmoud; Azadbakht, Azadeh

doi:10.1016/j.ab.2017.07.016

Cited by 93 publications

(52 citation statements)

References 31 publications

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“…Employing an aptamer proximity-binding assay approach, Ghanbari et al introduced an electrochemical aptasensor based on the functionalization of GCE surface by GQDs for the quantification of the hepatitis C virus (HCV) core antigen [330]. GQDs served as an appropriate substrate for aptamers via π-π stacking interactions to enrich the aptamer absorption on the GCE surface.…”

Section: Impedimetric Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

176

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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Section: Impedimetric Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

176

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“…111 Ghanbari et al developed an electrochemical aptasensor for sensitive detection of hepatitis C virus (HCV) core antigen ( Figure 3A). 112 At first, graphene quantum dots (GQD) were synthesized according to their previous work. 113 A glassy carbon electrode (GCE) was modified with GQD and the aptamer was adsorbed to the surface of GQDs due to the interactions between hydroxyl and carboxyl groups at GQDs edges and amine groups of the aptamer.…”

Section: Aptamer-based Sensorsmentioning

confidence: 99%

Review—Chemical and Biological Sensors for Viral Detection

Özer

Geiss

Henry

2019

J. Electrochem. Soc.

122

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Infectious diseases commonly occur in contaminated water, food, and bodily fluids and spread rapidly, resulting in death of humans and animals worldwide. Among infectious agents, viruses pose a serious threat to public health and global economy because they are often difficult to detect and their infections are hard to treat. Since it is crucial to develop rapid, accurate, cost-effective, and in-situ methods for early detection viruses, a variety of sensors have been reported so far. This review provides an overview of the recent developments in electrochemical sensors and biosensors for detecting viruses and use of these sensors on environmental, clinical and food monitoring. Electrochemical biosensors for determining viruses are divided into four main groups including nucleic acid-based, antibody-based, aptamer-based and antigen-based electrochemical biosensors. Finally, the drawbacks and advantages of each type of sensors are identified and discussed.

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“…Other research groups reported aptamers against HCV core (C) antigen for HCV detection. [109][110][111] Ghanbari et al immobilized an anti-C antigen aptamer onto graphene quantum dots (GOD), and the aptamer-GOD was placed on the surface of a glassy carbon electrode. 111 They demonstrated that the electrochemical aptamer-based biosensor effectively detects the HCV C antigen with the detection limit of 3.3 pg/mL.…”

Section: Hiv-1mentioning

confidence: 99%

“…[109][110][111] Ghanbari et al immobilized an anti-C antigen aptamer onto graphene quantum dots (GOD), and the aptamer-GOD was placed on the surface of a glassy carbon electrode. 111 They demonstrated that the electrochemical aptamer-based biosensor effectively detects the HCV C antigen with the detection limit of 3.3 pg/mL. 111 These aptamers against HCV E2 and C antigens are crucial for the early diagnosis of hepatitis C within the "diagnostic window period," when serum antibodies have not appeared yet.…”

Section: Hiv-1mentioning

confidence: 99%

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Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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Ultra-sensitive aptasensor based on a GQD nanocomposite for detection of hepatitis C virus core antigen

Cited by 93 publications

References 31 publications

Applications of Graphene Quantum Dots in Biomedical Sensors

Applications of Graphene Quantum Dots in Biomedical Sensors

Review—Chemical and Biological Sensors for Viral Detection

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

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