Ultra-Sensitive and Specific Detection of S. aureus Bacterial Cultures Using an Oligonucleotide Probe Integrated in a Lateral Flow-Based Device

Machado, Isabel Cristina Kasper; Goikoetxea, Garazi; Alday, Enara; Jiménez, Tania; Arias-Moreno, Xabier; Hernandez, Frank J.; Hernandez, Luiza I.

doi:10.3390/diagnostics11112022

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…21 Herein, we report on a novel biomarker for specific identification and targeting of S. pneumoniae using the nuclease activity associated with these bacteria. Nuclease activity has been proposed as an attractive biomarker for bacterial identification, 22,23 leveraging the catalytic function of nucleases to increase the sensitivity by degrading oligonucleotide substrates. This operational concept has been effectively applied to identify bacteria, cancer cells, and various human conditions.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent and electrochemical detection of nuclease activity associated with Streptococcus pneumoniae using specific oligonucleotide probes

Goikoetxea,

Akhtar,

Prysiazhniuk

et al. 2024

Analyst

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Section: Introductionmentioning

confidence: 99%

Fluorescent and electrochemical detection of nuclease activity associated with Streptococcus pneumoniae using specific oligonucleotide probes

Goikoetxea,

Akhtar,

Prysiazhniuk

et al. 2024

Analyst

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“…The lateral flow immunochromatographic kit (LFK) is a simple, rapid test that can be carried out in the field. Now the LFK technology is widely used for the determination of bacterial pathogens in clinical samples of different diseases, such as S. aureus in clinical samples [ 8 ], S. aureus enterotoxin A in milk [ 9 ], Vibrio cholera [ 10 ], Vibrio harveyi [ 11 ], Yersinia pestis in humans [ 12 ], Leptospira in urine [ 13 ], Helicobacter pylori in stool [ 14 ], Streptococcus suis serotype 2 [ 15 ], Salmonella enterica subsp. Enterica serovar Typhimurium ( S. typhimurium ) [ 16 , 17 ], Candida albicans [ 18 ], and COVID-19 coronavirus [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a lateral flow device for rapid simultaneous multiple detections of some common bacterial causes of bovine mastitis

Sayed¹,

Soliman²,

Elsaady³

2023

J Adv Vet Anim Res

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Objective: This work was conducted for the development of a 5-combi lateral flow immunochro¬matographic kit (LFK) for rapid and simultaneous identification of the common bacterial causes of bovine mastitis. The following pathogens are the identification targets of this kit: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus agalactiae, and Streptococcus pyogenes in milk samples from suspected bovine mastitis cases. The conventional microbiological identification of these agents is not only time-consuming and requires a fully equipped laboratory but also requires experienced personnel. Materials and Methods: Rabbit polyclonal antibodies (PAbs) specific to the antigenic components of the selected pathogens were prepared, and the pathogen-specific IgG was separated, purified, and conjugated with nanogold that was laid on the conjugate pad. Guinea pig PAbs specific to the microbial antigens of the selected pathogens were prepared, and their IgG content was separated, purified, and used as a capture antibody in the test (T) line on the nitrocellulose (NC) strips. Goat anti-rabbit IgG antibodies were used to capture the rabbit antibodies in the control (C) line of NC strips. The kit was held in a device comprising five strip-holding channels for the above-mentioned five bacterial species antigens. The developed LFK was evaluated, and its sensitivity and specificity were determined. Results: The developed kits were applied for the examination of bovine milk samples from suspected mastitis cases, and the average sensitivity, specificity, and accuracy of 5-combi LFK for the detection of the five selected bacterial species compared to bacteriological examination (gold standard test) were 93.90%, 80.83%, and 90.53%, respectively. The minimal microbial count that gave positive results using the developed LFK was 103 colony forming unit/ml. Treatment of the milk samples with an application buffer and its pre-incubation in trypticase soy broth for 6 h at 37°C before testing significantly increased the sensitivity of the prepared LFK. The developed kit proved simple and convenient, and the results could be obtained in less than 10 min.

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“…Due to the demand to quantitatively detect proteins at extremely low concentrations, versatile ultrasensitive technologies have been developed. − One of the assay technologies is immunomagnetic reduction (IMR). − …”

Section: Introductionmentioning

confidence: 99%

Development of a 36-Channel Instrument for Assaying Biomarkers of Ultralow Concentrations Utilizing Immunomagnetic Reduction

Chen¹,

Hsu²,

Lee³

et al. 2022

ACS Meas. Sci. Au

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With the demands of the high-throughput assay of biomarkers of ultralow concentrations in clinics, a 36-channel instrument utilizing immunomagnetic reduction (IMR) has been developed. The instrument involves the use of a high-T c superconducting-quantum-interference-device (SQUID) magnetometer to detect the signals due to the associations between target biomarker molecules and the antibody-functionalized magnetic nanoparticles in the reagent of IMR. In addition to illustrating the design and the measurements of the instrument, the assay characterizations for eight kinds of biomarkers related to neurodegenerative disease are investigated. Furthermore, the assay results among three independent instruments were compared. For an instrument, the channel-to-channel variations in measured concentrations of biomarkers are within a range of 2.09 to 5.62%. The assay accuracy was found to be from 99 to 103.7%. The p values in measured concentrations for any of the tested biomarkers were higher than 0.05 among the three instruments. The results demonstrate high throughput, high stability, and high consistency for the SQUID-IMR instruments.

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Ultra-Sensitive and Specific Detection of S. aureus Bacterial Cultures Using an Oligonucleotide Probe Integrated in a Lateral Flow-Based Device

Cited by 4 publications

References 27 publications

Fluorescent and electrochemical detection of nuclease activity associated with Streptococcus pneumoniae using specific oligonucleotide probes

Fluorescent and electrochemical detection of nuclease activity associated with Streptococcus pneumoniae using specific oligonucleotide probes

Development of a lateral flow device for rapid simultaneous multiple detections of some common bacterial causes of bovine mastitis

Development of a 36-Channel Instrument for Assaying Biomarkers of Ultralow Concentrations Utilizing Immunomagnetic Reduction

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