Ultra rapid liquid chromatography as second dimension in a comprehensive two-dimensional method for the screening of pharmaceutical samples in stability and stress studies

Huidobro, A.L.; Pruim, Peter; Schoenmakers, Peter J.; Barbas, Coral

doi:10.1016/j.chroma.2008.02.114

Cited by 62 publications

(25 citation statements)

References 18 publications

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“…Novel types of stationary phases and pump design are available that can withstand very high pressures (A500 bar) in so-called "ultra HPLC". Such systems are promising for the second dimension operation, as they allow essentially increased flow-rates and reduced separation times [22]. Unfortunately, such a system was not available in the present study.…”

Section: Introductionmentioning

confidence: 94%

“…4, showing the record of the second dimension UV detector, transformed into a 2-D contour plot as reported earlier [22], for three parallel gradient times, 280, 140 and 70 min. With a fixed sampling loop volume of 100 lL, the flow-rates in the two dimensions were increased in inverse proportion to the gradient time (25,50 and 100 lL /min in the first dimension and 1, 2 and 4 mL/min in the second dimension).…”

Section: Performance Of Lc6lc Systems With Various Alkyl Silica Colummentioning

confidence: 99%

“…These HPLC columns packed with particles with a thin porous shell (0.5 lm) exhibit high efficiency due to the shallow diffusion paths in the porous shell. Therefore in the present work we tested a C18 and a C8 porous shell column and compared them with fully porous and monolithic silica C18 columns used earlier for fast 2-D separations [22].…”

Section: Introductionmentioning

confidence: 99%

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Multidimensional LC×LC analysis of phenolic and flavone natural antioxidants with UV‐electrochemical coulometric and MS detection

Hájek

Škeříková

Česla

et al. 2008

J of Separation Science

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A comprehensive 2-D LC x LC system was developed for the separation of phenolic and flavone antioxidants, using a PEG-silica column in the first dimension and a C(18) column with porous-shell particles or a monolithic column in the second dimension. Combination of PEG and C18 or C8 stationary phase chemistries provide low selectivity correlations between the first dimension and the second dimension separation systems. This was evidenced by large differences in structural contributions to the retention by -COOH, -OH and other substituents on the basic phenol or flavone structure. Superficially porous columns with fused core particles or monolithic columns improve the resolution and speed of second dimension separation in comparison to a fully porous particle C(18) column. Increased peak capacity and high orthogonality in different 2-D setups was achieved by using gradients with matching profiles running in parallel in the two dimensions over the whole 2-D separation time range. Multi-dimensional set-up combining the LC x LC separation on-line with UV and multi-channel coulometric detection and off-line with MS/MS technique allowed positive peak identification. The Coularray software compensates for the effects of the baseline drift during the gradient elution and is compatible with parallel gradient comprehensive LC x LC technique. Furthermore, it provides significant improvement in the sensitivity and selectivity of detection in comparison to both UV and MS detection. The utility of these systems has been demonstrated in the analysis of beer samples.

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Section: Introductionmentioning

confidence: 94%

Section: Performance Of Lc6lc Systems With Various Alkyl Silica Colummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multidimensional LC×LC analysis of phenolic and flavone natural antioxidants with UV‐electrochemical coulometric and MS detection

Hájek

Škeříková

Česla

et al. 2008

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Using fast second dimension gradients (20-60 s, including the post-gradient column re-equilibration time), it was possible to accomplish on-line comprehensive LC × LC separations in 30-45 min [10][11][12]. Fast gradient separations within a fixed time improve at high linear flow velocities on short efficient columns, such as sub-2 m columns at very high operation pressures.…”

Section: Introductionmentioning

confidence: 98%

Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Jandera

Hájek

Česla

2011

Journal of Chromatography A

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“…Monolith columns were used in the second dimension and operated at a high flow rate of up to 10 mL min -1 [9,10]. Ultra-high pressure LC using a sub-2-lm stationary phase could carry out fast separation within 1 min [11,12]. The high-temperature LC technique was also used to construct a fast 2D-LC system [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Development of a Parallel-Tandem Column Interface in a Two-Dimensional Liquid Chromatography System

Zhang

Wang

et al. 2011

Chromatographia

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An ion exchange-reversed-phase two-dimensional liquid chromatography (2D-LC) system was developed based on a newly designed interface. The interface was constructed by alternately connecting tandem columns to one of the parallel separation columns to increase the separation efficiency. Separation on the tandem column does not affect the switching frequency; the valve switching could be maintained at high frequency. The constructed interface could be used in both ambient and elevated temperature modes. Under high-temperature mode, only the tandem column was heated, which simplified the operation, decreased the backpressure, improved the solvent compatibility, and speeded up the separation. Tryptic digestion of four proteins was used to evaluate the performance of the developed system. The peak capacity of the 2D-LC system reached 650 under ambient conditions and 890 under high temperature conditions. When compared with the traditional parallel column interface constructed in this paper, the new interface produced a 60% higher peak capacity.

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Ultra rapid liquid chromatography as second dimension in a comprehensive two-dimensional method for the screening of pharmaceutical samples in stability and stress studies

Cited by 62 publications

References 18 publications

Multidimensional LC×LC analysis of phenolic and flavone natural antioxidants with UV‐electrochemical coulometric and MS detection

Multidimensional LC×LC analysis of phenolic and flavone natural antioxidants with UV‐electrochemical coulometric and MS detection

Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Development of a Parallel-Tandem Column Interface in a Two-Dimensional Liquid Chromatography System

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