Ultra-performance liquid chromatography–tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide–DNA adducts

Feng, Feng; Wang, Xiaoli; Huang, Yuan; Wang, Hailin

doi:10.1016/j.jchromb.2009.05.054

Cited by 28 publications

(19 citation statements)

References 34 publications

(45 reference statements)

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“…1 B, showing typical autoradiography images, the major adduct band corresponds to (+)-anti-trans-BPDE-N2-dG, which is reported to be the most abundant DNA adduct. The other bands were (+)-anti-cis-BPDE-N2-dG and syn-BPDE-N2-dG, as reported previously [28]. BaP-DNA adduct was detected in HepG2 cells treated with BaP at concentrations higher than 0.5 M BaP.…”

Section: Effects Of Tcdd Pretreatment On the Bap-dna Adduct Formationsupporting

confidence: 87%

Dioxin suppresses benzo[a]pyrene-induced mutations and DNA adduct formation through cytochrome P450 1A1 induction and (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide inactivation in human hepatoma cells

Shiizaki¹,

Kawanishi²,

Yagi³

2013

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Effects Of Tcdd Pretreatment On the Bap-dna Adduct Formationsupporting

confidence: 87%

Dioxin suppresses benzo[a]pyrene-induced mutations and DNA adduct formation through cytochrome P450 1A1 induction and (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide inactivation in human hepatoma cells

Shiizaki¹,

Kawanishi²,

Yagi³

2013

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…DNA digestion was performed as described previously [36]. Genomic DNA (1 g) extracted from human sperms or cultured cells was digested with 1 U DNase I, 2 U CIP and 0.005 U snake venom phosphodiesterase I at 37 • C for 24 h. Microcon centrifugal filter device with a 3000 D cutoff membrane was used to remove protein from the digested DNA samples by centrifuging at 12,000 rpm for 60 min.…”

Section: Dna Enzymatic Digestionmentioning

confidence: 99%

Ultra-performance liquid chromatography/tandem mass spectrometry for accurate quantification of global DNA methylation in human sperms

Wang

Suo

Yin

et al. 2011

Journal of Chromatography B

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“…To calibrate the content of BPDE-DNA adducts in treated gDNA, one aliquot of genomic DNA was enzymatically digested into individual deoxynucleotides, and the BPDE-dG adduct was quantied by using stable isotope dilution LC-MS/MS analysis. 45 The estimated total frequency of BPDE-DNA adducts in gDNA was 5.8 adducts per 10 5 nucleotides, which was used as a BPDE-gDNA standard in this experiment.…”

Section: Lscm Uorescent Imaging Of Bpde-dna Adductsmentioning

confidence: 99%

Plastic antibody for DNA damage: fluorescent imaging of BPDE–dG adducts in genomic DNA

Yin

Wang

Song

et al. 2013

Analyst

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Exogenous and endogenous genotoxic carcinogens and their in vivo metabolites may result in DNA damage and cause adverse health effects. It is very difficult to specifically detect trace DNA damage in the presence of a large excess of unmodified nucleosides. Here we report a molecularly imprinted polymer (MIP) nanocomposite, namely nanoMIP, which can be used as a "plastic antibody" for specific recognition of benzo[a]pyrene diol epoxide (BPDE)-DNA adduct. Enhanced binding affinity (880 nM) of nanoMIPs was achieved by using two tailor-made functional monomers. The property of binding kinetics was greatly improved in virtue of the well-defined nanostructure, which was fabricated by initiators for continuous activator regeneration-atom transfer radical polymerization (ICAR-ATRP). The BPDE-adducted DNA can be specifically captured by synthetic nanoMIP. By taking advantage of this antibody-mimicking behavior, we further developed a fluorescently imaged particle counting immunoassay (FIPCIA) method for ultrasensitive detection of BPDE-ssDNA adducts using a laser scanning confocal microscope (LSCM). The number of countable fluorescent dots is proportional to the content of BPDE-ssDNA adducts in the DNA sample. The proposed plastic antibody-based FIPCIA method can detect traces of BPDE-DNA adducts as low as 18 pM in human lung carcinoma A549 cells.This highly-sensitive detection of DNA lesions offers a promising alternative to immunogenic antibodybased immunoassays for genomics and DNA modification analysis.

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Ultra-performance liquid chromatography–tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide–DNA adducts

Cited by 28 publications

References 34 publications

Dioxin suppresses benzo[a]pyrene-induced mutations and DNA adduct formation through cytochrome P450 1A1 induction and (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide inactivation in human hepatoma cells

Dioxin suppresses benzo[a]pyrene-induced mutations and DNA adduct formation through cytochrome P450 1A1 induction and (±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide inactivation in human hepatoma cells

Ultra-performance liquid chromatography/tandem mass spectrometry for accurate quantification of global DNA methylation in human sperms

Plastic antibody for DNA damage: fluorescent imaging of BPDE–dG adducts in genomic DNA

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