Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS)-based metabolomic analysis of mycelial biomass of three Ganoderma isolates from the Lower Volta River Basin of Ghana

Adotey, Gideon; Alolga, Raphael N.; Quarcoo, Abraham; Gedel, Mohammed Ahmed; Anang, Abraham K.; Holliday, John C.

doi:10.1016/j.jpba.2021.114355

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…Based on the MS and MS/MS data, peaks 1–6 were identified as ganoderic acid I, ganoderic acid C 2 , ganoderic acid G, ganoderic acid B, ganoderenic acid A, and ganoderic acid A, respectively. The data listed in Table 1 are consistent with the findings of previous studies, and the structures of the compounds are listed in Table 2 21–26 …”

Section: Resultssupporting

confidence: 89%

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Modeling and optimization of the protocol of complex chromatography separation of cyclooxygenase‐2 inhibitors from Ganoderma lucidum spore

Hou

et al. 2023

Phytochemical Analysis

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Introduction The spores of the medicinal fungus Ganoderma lucidum possess hepatoprotective properties. The main components, triterpenes, are particularly beneficial, making the screening and preparation of active triterpenes from Ganoderma lucidum significant. Objectives We aimed to screen and verify cyclooxygenase‐2 inhibitors from G. lucidum spores, establish a rapid online hyphenated technique for the preparation of active ingredients, and analyze the structures of the active ingredients. Methods Ultrafiltration LC combined with an enzyme inhibition assay and molecular docking was employed to screen and evaluate cyclooxygenase‐2 ligands, which were prepared by pressurized liquid extraction coupled online with countercurrent chromatography and semi‐preparative LC. The structures of the compounds were identified by nuclear magnetic resonance spectroscopy. Results Six cyclooxygenase‐2 inhibitors, namely, ganoderic acids I, C2, G, B, and A and ganoderenic acid A, were screened and evaluated. They were prepared using the online hyphenated technique, following which their structures were identified. Conclusion This study provides opportunities for the discovery and development of new therapeutic drugs from other natural resources, as the present instrumental setup achieved efficient and systematic extraction and isolation of natural products compared with reference separation methods, thus exhibiting significant potential for industrial applications.

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Section: Resultssupporting

confidence: 89%

“…The identification of the compounds obtained through PLE/CCC/SPLC was based on LC retention time, ESI/MS, 1 H‐NMR, and 13 C‐NMR data. The 1 H‐NMR and 13 C‐NMR data, which are provided in the Supporting Information, are in agreement with those of the corresponding compounds 21–26 …”

Section: Resultssupporting

confidence: 79%

Modeling and optimization of the protocol of complex chromatography separation of cyclooxygenase‐2 inhibitors from Ganoderma lucidum spore

Hou

et al. 2023

Phytochemical Analysis

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“…This current isolation of G. enigmaticum from the Lower Volta River Basin is very useful information and represents the second new record of G. enigmaticum in Ghana, although the third-ever isolation was made from Nigeria [30]. Recently, four lanostane triterpenoids were identified in the artificially cultivated mycelial biomass of G. enigmaticum from Ghana [35]. The identified lanostane compounds include ganoderic acid C6, ganoderenic acid D, ganoderenic acid A, and ganoderic acid G, together with ganoderenic acid K and ganoderenic acid AM1 as annotated compounds.…”

Section: Discussionmentioning

confidence: 75%

Molecular Identification and Characterization of Five Ganoderma Species from the Lower Volta River Basin of Ghana Based on Nuclear Ribosomal DNA (nrDNA) Sequences

Adotey,

Alolga,

Quarcoo

et al. 2023

JoF

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Ganoderma is a genus of biomedical fungus that is used in the development of numerous health products throughout the world. The Lower Volta River Basin of Ghana is an undulating land surface covered by extensive vegetation and water bodies and is rich in polypore mushrooms resembling various members of the Ganoderma genus. Despite the extensive biopharmaceutical benefits of Ganoderma spp., the isolates from the Lower Volta River Basin have not been properly characterized, thus limiting their use in the development of biotechnological products. In this study, Ganoderma spp. collected from the Lower Volta River Basin were genetically analyzed using the nuclear ribosomal sequences, the internal transcribed spacer 2 (ITS 2), the complete internal transcribed spacer (ITS), and the nuclear large subunit (nLSU). Blastn search and sequence analysis revealed that the sample we coded as Ganoderma LVRB-2 belongs to G. mbrekobenum, whereas Ganoderma LVRB-1, Ganoderma LVRB-14, and Ganoderma LVRB-16 belong to the species G. enigmaticum. Our analysis further demonstrates that Ganoderma LVRB-17 belongs to the species G. resinaceum. Thus, the five samples collected in the present study were positioned in three different distinct groups, namely G. mbrekobenum, G. enigmaticum, and G. resinaceum. The current data may serve as reference points for future studies.

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“…D. L. Liu et al (2015) revealed that ganoderenic acid B exhibited a potent reversal effect on multi‐drug resistance of HepG2/ADM cells to doxorubicin, vincristine and paclitaxel. Ganoderic acid C6 has been reported to be an antinociceptive component of G. lucidum (Adotey et al, 2021). Therefore, it can be inferred that the G. lucidum samples from Henan may have more bioactive effects.…”

Section: Resultsmentioning

confidence: 99%

Deciphering the chemical composition of Ganoderma lucidum from different geographical origins by mass spectrometry molecular networking coupled with multivariate analysis

Liu

Chen

Yang

et al. 2022

Biomedical Chromatography

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Ganoderma lucidum is a medicinal fungus that has been widely used in China and many Asian countries for thousands of years. This once rare macrofungus has now been artificially cultivated in a number of regions in China. However, detailed knowledge of its composition across different geographical origins is still lacking, as are analytical methods for comprehensive profiling of the diverse phytochemicals contained in G. lucidum. In this work, an on‐demand strategy based on high‐resolution MS and molecular networking is applied for natural product characterization, which led to the identification of 84 constituents in G. lucidum. Moreover, multivariate analysis, including hierarchical cluster analysis and orthogonal partial least squares‐discriminant analysis, was used to analyze the (dis)similarity of the G. lucidum samples collected from the three main production areas (i.e., Jilin, Henan and Shandong Province). The results revealed a significant variation in the chemical composition of samples from different provinces. Marker constituents corresponding to the differentiation were then screened in terms of the variable importance in projection value, P‐value and fold change. A total of 24 constituents were identified as geoherbalism markers, such as ganoderenic acid A for Henan, ganolucidic acid B for Jilin and ganodernoid D for Shandong. This proof‐of‐concept application demonstrates that combining MS molecular networking with meticulous multivariate analysis can provide a sensitive and comprehensive analytical approach for the quality assessment of traditional Chinese medicine ingredients. This study also suggests that the bioactivity and efficacy from different origins should be further evaluated considering the large difference in chemical compositions.

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Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS)-based metabolomic analysis of mycelial biomass of three Ganoderma isolates from the Lower Volta River Basin of Ghana

Cited by 5 publications

References 24 publications

Modeling and optimization of the protocol of complex chromatography separation of cyclooxygenase‐2 inhibitors from Ganoderma lucidum spore

Modeling and optimization of the protocol of complex chromatography separation of cyclooxygenase‐2 inhibitors from Ganoderma lucidum spore

Molecular Identification and Characterization of Five Ganoderma Species from the Lower Volta River Basin of Ghana Based on Nuclear Ribosomal DNA (nrDNA) Sequences

Deciphering the chemical composition of Ganoderma lucidum from different geographical origins by mass spectrometry molecular networking coupled with multivariate analysis

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