Ultra-performance liquid chromatography–mass spectrometry as a sensitive and powerful technology in lipidomic applications

Zhao, Ying-Yong; Wu, Shaoping; Liu, Shuman; Zhang, Yongmin; Lin, Rui‐Chao

doi:10.1016/j.cbi.2014.06.029

Cited by 129 publications

(88 citation statements)

References 96 publications

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“…Recently, an increased number of lipidomic studies and reviews have been published using mass spectrometry (MS), nuclear magnetic resonance (NMR), and other spectroscopic modalities [56][57][58][59][60][61]. Separation technologies, gas chromatography (GC), liquid chromatography (LC), super-critical fluid chromatography, and capillary electrophoresis, are critical for lipidomic examination of complex samples [62]. To obtain structural molecular ion information, low collision energy MS is first used followed by higher collision energy MS 2 conditions to obtain fragment ions.…”

Section: Lipidomicsmentioning

confidence: 99%

Lipidomics

Zhao

Vaziri

Lin

2015

Advances in Clinical Chemistry

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Section: Lipidomicsmentioning

confidence: 99%

Lipidomics

Zhao

Vaziri

Lin

2015

Advances in Clinical Chemistry

Self Cite

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“…Therefore, reduction of the complexity of lipid extracts is essential for reliable and accurate identification and quantification of individual species in the complex extract. LC-based lipidomics achieves this utilizing separation science in addition to enriching the low abundance species [33, 38]. In contrast, shotgun lipidomics is a high-throughput approach but at expense of preserving the complexity of a lipid extract because of the absence of pre-chromatographic separation.…”

Section: Lipidomics Workflow and Techniquesmentioning

confidence: 99%

Lipidomics: Techniques, Applications, and Outcomes Related to Biomedical Sciences

Yang

Han

2016

Trends in Biochemical Sciences

430

372

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Lipidomics is a newly emerged discipline that studies cellular lipids on a large scale based on analytical chemistry principles and technological tools, particularly mass spectrometry. Recently, techniques have greatly advanced and novel applications of lipidomics in the biomedical sciences have emerged. This review provides a timely update on these aspects. After briefly introducing the lipidomics discipline, we compare mass spectrometry-based techniques for analysis of lipids and summarize very recent applications of lipidomics in health and disease. Finally, we discuss the status of the field, future directions, and advantages and limitations of the field.

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“…UPLC operates with the use of sub-2 µm particles as packing materials in the analytical columns and specially designed instrumentation to withstand high pressure (6000–15 000 psi). 219 In this vein, the UPLC-MS technique has gained increased popularity in DNA adduct analysis in recent years for the reason that it can provide a wider range of flow rates while maintaining good chromatographic resolution and therefore more rapid analysis compared to conventional HPLC. For instance, Feng and co-workers 220 measured four diastereomers of anti-BPDE- N 2 -dG adducts on a UPLC-MS/MS system, where the separation time was shortened to 2–4 min and a low limit of detection of <0.7 fmol (s/n = 3) was achieved.…”

Section: Quantitative Analysis Of Dna Adductsmentioning

confidence: 99%

Mass spectrometry for the assessment of the occurrence and biological consequences of DNA adducts

2015

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Exogenous and endogenous sources of chemical species can react, directly or after metabolic activation, with DNA to yield DNA adducts. If not repaired, DNA adducts may compromise cellular functions by blocking DNA replication and/or inducing mutations. Unambiguous identification of the structures and accurate measurements of the levels of DNA adducts in cellular and tissue DNA constitute the first and important step towards understanding the biological consequences of these adducts. The advances in mass spectrometry (MS) instrumentation in the past 2–3 decades have rendered MS an important tool for structure elucidation, quantification, and revelation of the biological consequences of DNA adducts. In this review, we summarized the development of MS techniques on these fronts for DNA adduct analysis. We placed our emphasis of discussion on sample preparation, the combination of MS with gas chromatography-or liquid chromatography (LC)-based separation techniques for the quantitative measurement of DNA adducts, and the use of LC-MS along with molecular biology tools for understanding the human health consequences of DNA adducts. The applications of mass spectrometry-based DNA adduct analysis for predicting the therapeutic outcome of anti-cancer agents, for monitoring the human exposure to endogenous and environmental genotoxic agents, and for DNA repair studies were also discussed.

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Ultra-performance liquid chromatography–mass spectrometry as a sensitive and powerful technology in lipidomic applications

Cited by 129 publications

References 96 publications

Lipidomics

Lipidomics

Lipidomics: Techniques, Applications, and Outcomes Related to Biomedical Sciences

Mass spectrometry for the assessment of the occurrence and biological consequences of DNA adducts

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