Ultra‐performance liquid chromatography coupled with electrospray ionization/quadrupole‐time‐of‐flight mass spectrometry for rapid analysis of constituents of Suanzaoren decoction

A rapid, sensitive, and reliable approach for analyzing five kinds of erythrocyte phospholipids in Sprague-Dawley rats was provided by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry with MassLynx(TM) MassFragment. Improving conventional high performance liquid chromatography techniques, ultra high performance liquid chromatography integrated with quadrupole time-of-flight tandem mass spectrometry offers high sensitivity and increased analytical speed by using columns packed with sub-2 μm particles (1.7 μm), which allows a faster separation to be achieved. Through this method, 83 phospholipids were tentatively characterized based on their mass spectra and tandem mass spectra, as well as by matching the in-house formula database within a mass error of 5 ppm, including 40 phosphatidylcholines, 24 phosphatidyl ethanolamines, three phosphatidylinositols, six phosphatidylserines, and ten sphingomyelins. Our present results proved that the established method could be used to qualitatively analyze complex erythrocyte phospholipids in Sprague-Dawley rats and provide a useful data base for pharmacology and phospholipidomics to seek potential biomarkers of disease prediction.

Section: Introductionmentioning

confidence: 98%

Rapid identification of erythrocyte phospholipids in Sprague–Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Yang

et al. 2015

“…Recently, an LC Triple Quadrupole MS method using the multiple reaction monitoring pattern was developed for the simultaneous determination the bioactive constituents in TWHF extracts and its preparations [21]. Currently, LC-TOF-MS or UHPLC-TOF-MS has established a new platform for the online analysis of complex constituents in herbal medicines, with high sensitivity, short time, and low consumption of samples [22][23][24][25]. LC-TOF-MS can not only permit a quick and effective confirmation of the elemental composition of the ions, but can also provide high specificity and selectivity in the determination of the compounds in complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of multiple bioactive constituents in tripterygium glycosides tablets using liquid chromatography coupled with time‐of‐flight mass spectrometry

Zhou

Lan

et al. 2015

A simultaneous determination method based on liquid chromatography coupled with time-of-flight mass spectrometry was developed for the analysis of 11 bioactive constituents in tripterygium glycosides tablets, an immune and inflammatory prescription used in China. The analysis was fully optimized on a 1.8 μm particle size C18 column with linear gradient elution, permitting good separation of the 11 analytes and two internal standards in 21 min. The quantitation of each target constituent was carried out using the narrow window extracted ion chromatograms with a ±l0 ppm extraction window, yielding good linearity (r(2) > 0.996) with a linear range of 10-1000 ng/mL. The limits of quantitation were low ranging from 0.25 to 5.02 ng/mL for the 11 analytes, and the precisions and repeatability were better than 1.6 and 5.3%, respectively. The acceptable recoveries obtained were in the range of 93.4-107.4%. This proposed method was successfully applied to quantify the 11 bioactive constituents in commercial samples produced by nine pharmaceutical manufacturers to profile the quality of these preparations. The overall results demonstrate that the contents of the 11 bioactive constituents in different samples were in great diversity, therefore, the quality, clinical safety, and efficacy of this drug needs further research and evaluation.

“…A TCM formula usually contains several herbs in different proportions and exerts its therapeutic effects via a multicomponent and multitargeted approach . However, it is difficult to investigate the mechanism of actions of each individual component without isolation and characterization of such bioactive component, it would be impossible to access the mechanism of action of a complex TCM formula . Thus, the development of a reliable and rapid analytical method, such as UHPLC–MS and UHPLC–ESI‐Q‐TOF‐MS, to facilitate the chemical identification of a complex mixture of TCM would be important.…”

Section: Introductionmentioning

confidence: 99%

Systematic chemical profiling of a multicomponent Chinese herbal formula Huo Luo Xiao Ling Dan by ultra high performance liquid chromatography coupled with electrospray ionization quadrupoletime‐of‐flight mass spectrometry

Wang

et al. 2015

Huo Luo Xiao Ling Dan, a Chinese herbal formula consisting of 11 different herbs, has been used in folk medicine for the treatment of arthritis and other chronic inflammatory diseases. However, the chemical compositions of Huo Luo Xiao Ling Dan are not completely characterized. In the present study, an ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry method in positive and negative ion modes was employed to identify biochemical constitutes in Huo Luo Xiao Ling Dan. As a result, a total of 76 compounds including alkaloids, monoterpene glycosides, iridoids, phenolic acids, and tanshinones, coumarins, lactones, flavones, and their glycosides, triterpenes, and triterpene saponins were characterized by comparing the retention time and mass spectrometry data with reference standards within 5 ppm error or by reference to the reference literature. These results would provide the basis for a further in vivo study of Huo Luo Xiao Ling Dan and information for potential new drug candidates for treating arthritis and other chronic inflammatory diseases.