Ultra‐performance convergence chromatography with tandem mass spectrometry‐assisted method for rapid enantioseparation and determination of fluazifop‐butyl in tobacco and soil

Abstract

A quick, green, and sensitive method for chiral separation and determination of fluazifop-butyl enantiomers in tobacco and soil was established by ultraperformance convergence chromatography with tandem mass spectrometry (UPC 2 -MS/MS). The baseline separation was obtained on an ACQUITY UPC 2 Trefoil CEL2 column in 4 minutes with CO 2 and methanol as mobile phase. Column temperature, auto back pressure regulator pressure (ABPR), and modifier solvent were optimized to obtain the best separation efficiency.Under the optimal conditions, the recoveries of both enantiomers were 82.8% to 99.5% with relative standard deviations (RSDs) less than 5.5% at three different concentration levels in two matrices. Good coefficients of determination (R 2 ≥ 0.9976) were achieved over the concentration range of 10 to 500 ng/mL. The limits of detection (LODs) for all enantiomers in the two matrices varied from 1.6 to 2.1 μg/kg, and the limits of quantification (LOQs) did not exceed 7.0 μg/kg. The proposed method was then successfully applied to analyze authentic samples, confirming that it was a green, convenient, and reliable strategy for the analysis of fluazifop-butyl enantiomers in tobacco and soil.

Simultaneous determination of guaifenesin enantiomers and ambroxol HCl using 50‐mm chiral column for a negligible environmental impact

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An alternative strategy based on ultra‐high‐performance supercritical fluid chromatography for full monosaccharide compositional analysis of polysaccharides in Schisandra chinensis fruits

“…The high-density diol surface bonded stationary phase of the Torus DIOL column can effectively improve the overall stability of analytical methods [20]. In addition, Trefoil AMY stationary phases and Viridis BEH 2-EP column with optimal bed density filling technology have considerable retention, peak shape, and selectivity for most compounds [20,21].…”

Analytical methods to analyze pesticides and herbicides

“…Mean recoveries of 74.6%-108.1%, with intra-day RSDs (0.9%-18.3%) and inter-day RSDs < 13.8%, were also realized. Yang, Tang, et al (2019) utilized ultra-performance convergence chromatography with tandem mass spectrometry (UPC2-MS/MS) to determine fluazifop-butyl in tobacco and soil. The method had recoveries of 82.8%-99.5% (RSDs < 5.5%) for the target compounds at three levels in both matrices.…”