Ultra-low aspect ratio spiral microchannel with ordered micro-bars for flow-rate insensitive blood plasma extraction

Shen, Shaofei; Zhang, Fangjuan; Wang, Shuting; Wang, Jiangran; Long, Dandan; Wang, Defu

doi:10.1016/j.snb.2019.02.066

Cited by 36 publications

(39 citation statements)

References 32 publications

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“…Therefore, a low-aspect-ratio (AR) channel should be developed instead of a high-AR channel. Our group recently established a unique approach using ultra-low-AR microchannels for regulating the Dean vortex to achieve highly efficient fluid mixing and cell manipulation [ 37 , 38 , 39 ]. The systematic investigation and demonstration of multi-vortexes in vertical and horizontal planes have been less advanced.…”

Section: Introductionmentioning

confidence: 99%

Numerical Study of Multivortex Regulation in Curved Microchannels with Ultra-Low-Aspect-Ratio

et al. 2021

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The field of inertial microfluidics has been significantly advanced in terms of application to fluid manipulation for biological analysis, materials synthesis, and chemical process control. Because of their superior benefits such as high-throughput, simplicity, and accurate manipulation, inertial microfluidics designs incorporating channel geometries generating Dean vortexes and helical vortexes have been studied extensively. However, existing technologies have not been studied by designing low-aspect-ratio microchannels to produce multi-vortexes. In this study, an inertial microfluidic device was developed, allowing the generation and regulation of the Dean vortex and helical vortex through the introduction of micro-obstacles in a semicircular microchannel with ultra-low aspect ratio. Multi-vortex formations in the vertical and horizontal planes of four dimension-confined curved channels were analyzed at different flow rates. Moreover, the regulation mechanisms of the multi-vortex were studied systematically by altering the micro-obstacle length and channel height. Through numerical simulation, the regulation of dimensional confinement in the microchannel is verified to induce the Dean vortex and helical vortex with different magnitudes and distributions. The results provide insights into the geometry-induced secondary flow mechanism, which can inspire simple and easily built planar 2D microchannel systems with low-aspect-ratio design with application in fluid manipulations for chemical engineering and bioengineering.

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Section: Introductionmentioning

confidence: 99%

Numerical Study of Multivortex Regulation in Curved Microchannels with Ultra-Low-Aspect-Ratio

et al. 2021

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“…25 As perhaps the most successful advanced microchannel design, curved microfluidic channels create a relatively strong and stable secondary flow. This type of microchannel design can be categorized as either spiral 10,[26][27][28][29][30] or asymmetric curving channels 31,32 (sigmoidal channels with alternating curvature and serpentines). Due to the centrifugal forces present in curved channels, fluid tends to flow from the inner wall toward the outer wall, returning through the regions near the channel's top and bottom walls.…”

Section: Introductionmentioning

confidence: 99%

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

et al. 2019

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Curved microchannels allow controllable microparticle focusing, but a full understanding of particle behavior has been limited-even for simple rectangular and trapezoidal shapes. At present, most microfluidic particle separation literature is dedicated to adding "internal" complexity (via sheath flow or obstructions) to relatively simple cross-sectional channel shapes. We propose that, with sufficient understanding of particle behavior, an equally viable pathway for microparticle focusing could utilize complex "external" cross-sectional shapes. By investigating three novel, complex spiral microchannels, we have found that it is possible to passively focus (6, 10, and 13 μm) microparticles in the middle of a convex channel. Also, we found that in concave and jagged channel designs, it is possible to create multiple, tight focusing bands. In addition to these performance benefits, we report an "additive rule" herein, which states that complex channels can be considered as multiple, independent, simple cross-sectional shapes. We show with experimental and numerical analysis that this new additive rule can accurately predict particle behavior in complex cross-sectional shaped channels and that it can help to extract general inertial focusing tendencies for suspended particles in curved channels. Overall, this work provides simple, yet reliable, guidelines for the design of advanced curved microchannel cross sections.

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“…Dean drag forces can accelerate the focusing process [ 153 ], so the spiral microchannel design benefits from a smaller device footprint. Various channel cross-sections (e.g., low-aspect-ratio rectangular and trapezoidal) and various microfeatures (e.g., micropillar filters, microbar obstacles) were employed to further enhance plasma separation [ 151 , 152 , 153 , 156 , 157 , 158 ].…”

Section: Endogenous Targetsmentioning

confidence: 99%

“…By combining a spiral microchannel with equally spaced microbar obstacles, blood cells were more effectively focused into a single stream [ 153 ]. Through systematic optimization process, the authors had chosen 450 μm × 200 μm microbars and π/15 angle between neighboring microbars [ 157 ] and purify plasma from 15× diluted blood with high purity (99.99%) and throughput (up to 15 mL/min, Figure 9 c). This system’s ability to tolerate variations in operational flow rates enabled plasma extraction using a hand-operated syringe with 99.99% purity and 67.57% of plasma yield.…”

Section: Endogenous Targetsmentioning

confidence: 99%

Inertial Microfluidics Enabling Clinical Research

et al. 2021

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Fast and accurate interrogation of complex samples containing diseased cells or pathogens is important to make informed decisions on clinical and public health issues. Inertial microfluidics has been increasingly employed for such investigations to isolate target bioparticles from liquid samples with size and/or deformability-based manipulation. This phenomenon is especially useful for the clinic, owing to its rapid, label-free nature of target enrichment that enables further downstream assays. Inertial microfluidics leverages the principle of inertial focusing, which relies on the balance of inertial and viscous forces on particles to align them into size-dependent laminar streamlines. Several distinct microfluidic channel geometries (e.g., straight, curved, spiral, contraction-expansion array) have been optimized to achieve inertial focusing for a variety of purposes, including particle purification and enrichment, solution exchange, and particle alignment for on-chip assays. In this review, we will discuss how inertial microfluidics technology has contributed to improving accuracy of various assays to provide clinically relevant information. This comprehensive review expands upon studies examining both endogenous and exogenous targets from real-world samples, highlights notable hybrid devices with dual functions, and comments on the evolving outlook of the field.

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Ultra-low aspect ratio spiral microchannel with ordered micro-bars for flow-rate insensitive blood plasma extraction

Cited by 36 publications

References 32 publications

Numerical Study of Multivortex Regulation in Curved Microchannels with Ultra-Low-Aspect-Ratio

Numerical Study of Multivortex Regulation in Curved Microchannels with Ultra-Low-Aspect-Ratio

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

Inertial Microfluidics Enabling Clinical Research

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