Ultra-high-throughput SPE-MALDI workflow: Blueprint for efficient purification and screening of peptide libraries

Identification of dipeptides by MALDI-ToF mass spectrometry in long-processing Spanish dry-cured ham

“…A further point is that MALDI-ToF analysis shows high potential for faster peptide identification when coupled with separation techniques. Actually, ultra-high-throughput-solid-phase extraction (SPE)-RP-ultra performance liquid chromatography (UPLC)- electrospray ionization (ESI)-MALDI-ToF was proposed as an analytical workflow to support the rapid analysis of large SPE-purified peptide libraries ( Bennett et al, 2021 ). On the other hand, MALDI-ToF has also been used in the detection of food adulterations and characterization of food allergens ( Calvano, Bianco, Losito, & Cataldi, 2021 ).…”

High‐Throughput Platform for Novel Reaction Discovery

“…[7] Notably, ionexchange solid-phase extraction (SPE) methods with cartridges or 96-well microtiter plates have greatly facilitated high-throughput syntheses of new molecules. [8] Quaternary ammonium (QA) groups have a strong ionic character and carry a permanent charge that is not affected by the pH of the solution. Among the different types of ion-exchange resins, weak cation exchange (WCX) resins offer enhanced selectivity for QA-containing molecules.…”

“…Based on electrostatic interactions between sorbents and analytes of interest, [6] ion‐exchange resins are widely used in organic synthesis to purify reaction mixtures [7] . Notably, ion‐exchange solid‐phase extraction (SPE) methods with cartridges or 96‐well microtiter plates have greatly facilitated high‐throughput syntheses of new molecules [8] . Quaternary ammonium (QA) groups have a strong ionic character and carry a permanent charge that is not affected by the pH of the solution.…”

Rapid antibody conformational screening by matrix‐assisted laser desorption/ionization hydrogen‐deuterium exchange mass spectrometry

“…Areas of the protein that are involved in extensive hydrogen bonding networks or are physically occluded from the surrounding solvent and, exchange at a slower rate relative to highly exposed or labile hydrogens [11]. Our group has previously reported methods combining the advantages of HDX-MS with the speed of MALDI to rapidly analyze peptide and protein conformation [12][13][14]. However, this prior work focused on smaller proteins and peptides and must be adapted to suit the analytical and high-throughput needs of analyzing ADCs, BsAbs, and mAbs produced from cell-development efforts.…”