Ultra high performance liquid chromatography-quadrupole-time of flight analysis for the identification and the determination of resveratrol and its metabolites in mouse plasma

Menet, Marie-Claude; Cottart, Charles‐Henry; Taghi, Méryam; Nivet-Antoine, Valérie; Dargère, Delphine; Vibert, Françoise; Laprévôte, Olivier; Beaudeux, Jean-Louis

doi:10.1016/j.aca.2012.11.048

Cited by 16 publications

(29 citation statements)

References 37 publications

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“…The method used to quantify resveratrol and its sulfo- and glucurono-derivatives has been previously validated in mouse plasma and has been described in detail elsewhere [11]. Briefly, initial standard-stock solutions of trans -resveratrol, trans -resveratrol-4′-O-ß-D-glucuronide, trans -resveratrol-3-O-ß-D-glucuronide, and trans -resveratrol-3-sulfate, as well as initial corresponding internal standard-stock solutions, were prepared in MeOH at 5 mg.mL −1 .…”

Section: Methodsmentioning

confidence: 99%

“…Plasma samples were prepared according to Menet et al [11]. Briefly, 50 µL of untreated mouse plasma spiking by analyte standards or 50 µL of treated lemur plasma, were vortex-mixed with the internal standard solution and 200 µL of ACN for 20 s. They were then let to stand for 20 min and centrifuged at 1000 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The conditions for analyses were the same as described by Menet et al [11]. Samples were injected into an Acquity UPLC system (Waters, Manchester, UK), equipped with an Acquity UHPLC BEH C18 column (50×2.1 mm inner diameter, particle size 1.7 µm), which was purchased from Waters (Guyancourt, France).…”

Section: Methodsmentioning

confidence: 99%

“…However, the sulfo- and glucurono-metabolites of resveratrol are mainly represented in plasma and could sustain their own activities, although resveratrol metabolism may vary from one species to another [10]. Up until now, the identification and assessment of resveratrol metabolites has been performed in several species, such as the mouse [11], rat [12], dog [13], pig [14], and humans [15], [16].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the present study was to identify and assess resveratrol metabolites in the plasma of mouse lemurs after oral intake of 200 mg.kg −1 of resveratrol, using ultra-high performance liquid chromatography (UHPLC) coupled to a high-resolution mass-spectrometer, quadrupole-time of flight (Q-TOF) [11].…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur (Microcebus murinus), Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of Flight

et al. 2014

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The grey mouse lemur (Microcebus murinus) is a non-human primate used to study the ageing process. Resveratrol is a polyphenol that may increase lifespan by delaying age-associated pathologies. However, no information about resveratrol absorption and metabolism is available for this primate. Resveratrol and its metabolites were qualitatively and quantitatively analyzed in male mouse-lemur plasma (after 200 mg.kg−1 of oral resveratrol) by ultra-high performance liquid chromatography (UHPLC), coupled to a quadrupole-time-of-flight (Q-TOF) mass spectrometer used in full-scan mode. Data analyses showed, in MSE mode, an ion common to resveratrol and all its metabolites: m/z 227.072, and an ion common to dihydro-resveratrol metabolites: m/z 229.08. A semi-targeted study enabled us to identify six hydrophilic resveratrol metabolites (one diglucurono-conjugated, two monoglucurono-conjugated, one monosulfo-conjugated and two both sulfo- and glucurono-conjugated derivatives) and three hydrophilic metabolites of dihydro-resveratrol (one monoglucurono-conjugated, one monosulfo-conjugated, and one both sulfo- and glucurono-conjugated derivatives). The presence of such metabolites has been already detected in the mouse, rat, pig, and humans. Free resveratrol was measurable for several hours in mouse-lemur plasma, and its two main metabolites were trans-resveratrol-3-O-glucuronide and trans-resveratrol-3-sulfate. Free dihydro-resveratrol was not measurable whatever the time of plasma collection, while its hydrophilic metabolites were present at 24 h after intake. These data will help us interpret the effect of resveratrol in mouse lemurs and provide further information on the inter-species characteristics of resveratrol metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur (Microcebus murinus), Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of Flight

et al. 2014

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Distribution of trans‐resveratrol and its metabolites after acute or sustained administration in mouse heart, brain, and liver

Menet

Baron

Taghi

et al. 2017

Molecular Nutrition Food Res

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Scope Trans‐resveratrol is widely studied for its potentially beneficial effects on numerous disorders. It is rapidly metabolized and its metabolites can exhibit biological activity. The present study aimed to investigate whether acute or sustained trans‐resveratrol administration impacted on the distribution of trans‐resveratrol and its metabolites in brain, heart, and liver. Methods and results We used ultra‐HPLC quadrupole‐TOF (UHPLC‐Q‐TOF) in a full‐scan mode to identify and assess large numbers of resveratrol metabolites. For acute intake, mice were overfed with a single dose of trans‐resveratrol (150 mg/kg) and organs were collected after 30 and 60 min. For sustained intake, trans‐resveratrol was given in the chow (0.04% w/w corresponding to 40 mg/kg/day), and plasma and the organs were collected after 3 months of this resveratrol diet. We found that trans‐resveratrol‐3‐O‐glucuronide and resveratrol‐3‐sulfate were the main metabolites found after acute intake, and free trans‐resveratrol (in the brain and heart) and dihydroresveratrol derivatives were found after sustained administration Conclusions Our results show notable differences between acute and sustained administration of trans‐resveratrol and distribution of trans‐resveratrol and its metabolites in mouse heart, brain, and liver. The results suggest a strategy for development of galenic forms of resveratrol.

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Quantification of trans-resveratrol and its metabolites in human plasma using ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry

Svilar

Martin

Defoort

et al. 2019

Journal of Chromatography B

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Ultra high performance liquid chromatography-quadrupole-time of flight analysis for the identification and the determination of resveratrol and its metabolites in mouse plasma

Cited by 16 publications

References 37 publications

Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur (Microcebus murinus), Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of Flight

Resveratrol Metabolism in a Non-Human Primate, the Grey Mouse Lemur (Microcebus murinus), Using Ultra-High-Performance Liquid Chromatography–Quadrupole Time of Flight

Distribution of trans‐resveratrol and its metabolites after acute or sustained administration in mouse heart, brain, and liver

Quantification of trans-resveratrol and its metabolites in human plasma using ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry

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