Ultra high performance liquid chromatography tandem mass spectrometry analysis of quorum-sensing molecules of Candida albicans

Greguš, Petr; Vlčková, Hana; Buchta, Vladimı́r; Kestřánek, Jan; Krivcíková, Lucie; Nováková, Lucie

doi:10.1016/j.jpba.2010.05.029

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…The C. albicans quorum sensing molecules farnesol and tyrosol are via a LC-MS/MS method quantifiable with limits of detection of respectively 15.2 and 3.0 nM (Gregus et al, 2010). …”

Section: Analytical Techniquesmentioning

confidence: 99%

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

et al. 2017

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The expression of certain bacterial genes is regulated in a cell-density dependent way, a phenomenon called quorum sensing. Both Gram-negative and Gram-positive bacteria use this type of communication, though the signal molecules (auto-inducers) used by them differ between both groups: Gram-negative bacteria use predominantly N-acyl homoserine lacton (AHL) molecules (autoinducer-1, AI-1) while Gram-positive bacteria use mainly peptides (autoinducer peptides, AIP or quorum sensing peptides). These quorum sensing molecules are not only involved in the inter-microbial communication, but can also possibly cross-talk directly or indirectly with their host. This review summarizes the currently applied analytical approaches for quorum sensing identification and quantification with additionally summarizing the experimentally found in vivo concentrations of these molecules in humans.

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“…The C. albicans quorum sensing molecules farnesol and tyrosol are via a LC-MS/MS method quantifiable with limits of detection of respectively 15.2 and 3.0 nM (Gregus et al, 2010). …”

Section: Analytical Techniquesmentioning

confidence: 99%

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

et al. 2017

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“…The results of multiple regression analysis (Table 3), canonical correlation analysis (Table 4), and advanced regression models (Fig. 2) confirmed that the presence of symptoms, hrHPV DNA, and Ureaplasma urealyticum are the predictor variables with the highest influence on the formation of the [44] tested the samples of vaginal washing from the women with/without of symptoms of vulvovaginosis. Obtained results showed the presence of tyrosol and/ or farnesol aromatic alcohols in 10 of 12 tested samples.…”

Section: Determination Of 2-phenylethanol In Vivomentioning

confidence: 57%

“…All those alcohols seem to have important influence on the dynamics of growth and morphogenesis in Candida species. 2-phenylethanol and tryptophol at high concentrations (C500 lM) inhibit the filamentous growth of Candida and biofilm formation, while tyrosol stimulate/ promote both effects [44]. Martins et al [48] identified isoamyl alcohol, 2-phenylethanol, E-nerolidol, and E, E-farnesol in the extracellular medium of planktonic and biofilm cells of C. albicans.…”

Section: Determination Of 2-phenylethanol In Vivomentioning

confidence: 99%

The correlation of the lifestyle and medical conditions with the vaginal infections and production of 2-phenylethanol

Findri-Guštek¹,

Petek

Sarajlija

et al. 2012

Arch Gynecol Obstet

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The medical exposure to irradiation, marital status, and number of partners as well as stress factors (miscarriages, chronic, viral, or tumor illnesses) had the highest influence on the development of the bacterial/fungal/viral infections. The formation of 2-phenylethanol, both in vivo and in vitro, was confirmed and connected with Candida species. Besides, according to statistical tests, it seems that presence of symptoms, hrHPV DNA, and Ureaplasma urealyticum had also significant role on the formation of 2-phenylethanol in vivo.

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“…La base de este control celular dependiente de la densidad de la morfogénesis, es similar a la que se observa con las células bacterianas que regulan sus actividades a través de la detección de quorum. Se han identificado un número de moléculas que afectan la capacidad de Candida albicans de sufrir el cambio de levadura a hifas, y tres compuestos: Tyrosol, farnesol, y ácido farnesóico, demostraron ser moléculas autoinductoras del sistema de QS [29,30,31,32]. Por otra parte se ha demostrado la producción de farnesol en un número de Cándida aislada en laboratorios clínicos.…”

Section: Mecanismo De Quorum -Sensing Y Su Efecto En La Patogénesis Dunclassified

Quorum sensing: mecanismo de comunicación celular en “Candida albicans”

Aparicio-Marenco

Daza

Trujillo

et al. 2013

Cienc. Salud virtual

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El presente artículo es una revisión sobre quórum-sensing como sistema de regulación dependiente de la acumulación en el medio ambiente de unas moléculas señal, denominadas autoinductores, sintetizadas durante el crecimiento microbiano, difundidas al exterior, para alcanza un umbral y generar de este modo una respuesta adaptativa. Microorganismos como Cándida habitante de la cavidad oral, tracto gastrointestinal y vagina, constituyen los agentes etiológicos de micosis oportunistas por excelencia, ocasionando mas del 50% de las infecciones fungicas a causa del uso indiscriminado de antibióticos de amplio espectro, la implantación de material protésico y de órganos, la corticoterapia e inmunosupresión terapéutica o adquirida, registrandose un aumento significativo de cuadros clínicos producidos por levaduras en los últimos años, con diversas manifestaciones dependientes de la localicacion de la infección y tipo de paciente. Recientemente, se ha demostrado que Candida albicans regula su transformación morfogénico a través de cambios en la densidad celular implicados en su poder patogenico utilizando moléculas señal como farnesol, tyrosol y ácido farnesóico. Es así, como en años recientes han sido publicadas diversas investigaciones orientadas a dilucidar los mecanismos de comunicacion microbiana mediante autoinductores. Por tal, el objeto de ésta revisión de literatura científica, tiene por objeto describir aspectos relacionados con los antecedentes históricos y el esclarecimiento acerca de la manera como C albicans utiliza éste mecanismo para la inducción de cambios morfogénicos por la sintesis de sustancias como farnesol, mediante la búsqueda, recopilación y revisión de artículos de publicación reciente en diversas bases de datos de carácter científico y médico.

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Ultra high performance liquid chromatography tandem mass spectrometry analysis of quorum-sensing molecules of Candida albicans

Cited by 17 publications

References 21 publications

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo

The correlation of the lifestyle and medical conditions with the vaginal infections and production of 2-phenylethanol

Quorum sensing: mecanismo de comunicación celular en “Candida albicans”

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