Ultra‐Fast Single‐Nucleotide‐Variation Detection Enabled by Argonaute‐Mediated Transistor Platform

Abstract

Abstract“Test‐and‐go” single‐nucleotide variation (SNV) detection within several minutes remains challenging, especially in low‐abundance samples, since existing methods face a trade‐off between sensitivity and testing speed. Sensitive detection usually relies on complex and time‐consuming nucleic acid amplification or sequencing. Here, we develop a graphene field‐effect transistor (GFET) platform mediated by Argonaute protein that enables rapid, sensitive and specific SNV detection. The Argonaute protein provides a nanoscale binding channel to preorganize the DNA probe, accelerating target binding and rapidly recognizing SNVs with single‐nucleotide resolution in unamplified tumor‐associated microRNA, circulating tumor DNA, virus RNA and reverse transcribed cDNA when a mismatch occurs in the seed region. An integrated microchip simultaneously detects multiple SNVs in agreement with sequencing results within 5 min, achieving the fastest SNV detection in a “test‐and‐go” manner without the requirement of nucleic acid extraction, reverse transcription and amplification.This article is protected by copyright. All rights reserved

Sensitivity-Enhancing Strategies of Graphene Field-Effect Transistor Biosensors for Biomarker Detection

“…The functionalization step is crucial for constructing high-performance GFET biosensors . The main strategies used to functionalize GFET-based biosensors include the functionalization of the graphene channel , or the gate electrode surface. , …”