Ulmus macrocarpa Hance Extracts Attenuated H2O2 and UVB-Induced Skin Photo-Aging by Activating Antioxidant Enzymes and Inhibiting MAPK Pathways

Abstract

To protect from reactive oxygen species (ROS) damages, skin cells have evolved to have antioxidant enzymes, such as copper and zinc-dependent superoxide dismutase (SOD1), mitochondrial manganese-dependent superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR), and suppressed the expression of matrix metalloproteinases (MMPs) through the mitogen-activated protein kinase (MAPK) signaling pathways, such as c-Jun N-terminal kinase (JNK) and p38. Bioactive compounds analyses were performed using a high-performance liquid chromatography-photodiode array detector (HPLC-PDA) system. The antioxidant activity of Ulmus macrocarpa Hance (UMH) extracts was estimated in vitro. The anti-aging activity of UMH extracts was estimated in vivo using the SKH-1 hairless mice. The UMH extracts reduced the H2O2-induced intracellular ROS production and the cell damages in human dermal fibroblasts (HDFs). Moreover, the H2O2-induced phosphorylation of JNK and p38 was detected in HDF and UMH extracts blocked the phosphorylation. These results suggest that UMH extracts can reduce the expression of MMPs and the reduced MMPs lead to the inhibition of collagen degradation. In addition, oral administration of the UMH extracts decreased the depth, thickness, and length of wrinkles on UVB exposed hairless mice. Therefore, UMH extracts play an advantage of the functional materials in antioxidant and anti-aging of skin.

Dihydrocaffeic Acid Prevents UVB-Induced Oxidative Stress Leading to the Inhibition of Apoptosis and MMP-1 Expression via p38 Signaling Pathway

“…Human skin provides the main protection barrier against environmental agents, as well as conferring external beauty. Extrinsic skin aging, also referred as to skin photoaging, occurs due to the cumulative exposure to external stimuli, and UVA and UVB irradiation is one of the most harmful factors [31]. UVB promotes oxidative stress by increasing ROS production and decreasing endogenous antioxidants, directly causing molecular damage and activating aging-related signal transduction.…”

“…UVB promotes oxidative stress by increasing ROS production and decreasing endogenous antioxidants, directly causing molecular damage and activating aging-related signal transduction. In addition to photoaging, the alterations induced by UVB also can lead to other skin disorders, including skin cancer [5, 31].…”

“…These set of alterations lead to ECM degradation and the appearance of photoaging signals [41]. It is well known that antioxidant extracts or compounds that inhibit MMP expression can be used to prevent skin aging [31]. Previous research has already shown that DHCA decreases MMP-2 and MMP-9 inhibition in the brain tissue of rats [12].…”

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

“…Several evidence suggests that p90RSK is activated by MAPK ( 61 ). The activation of MAPK signaling pathway will increase antioxidant activities ( 62 ) properties. Furthermore, increasing antioxidant activity and activating MAPK signaling will result in the apoptosis of CC cells ( 63 ).…”

Rapamycin Protects Skin Fibroblasts from Ultraviolet B-Induced Photoaging by Suppressing the Production of Reactive Oxygen Species

“…Enhanced ROS levels induce the production of matrix metalloproteinases (MMPs), which can degrade extracellular matrix components and inhibit collagen synthesis, causing skin relaxation, wrinkles, and erythema [9]. Additionally, excessive ROS levels can disrupt the balance of the oxidation/antioxidant system, resulting in the dysregulated secretion of antioxidants, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) [10-13]. Given the important role of ROS, reducing ROS accumulation is a future approach for skin photoprotection.…”