Ulcerative Lesions and Diarrhea: Answer

Beuerlein, Katherine G; Bowers, Nathan L.; Savas, Jessica; Strowd, Lindsay C.

doi:10.1097/dad.0000000000002081

Cited by 1 publication

(1 citation statement)

References 27 publications

(43 reference statements)

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“… 14 , 15 However, patients with UC are often accompanied by diarrhea, leading to the short residence time of drugs in the colon and the inability to exert their therapeutic effects fully. 16 , 17 Nano drug delivery system (NDDS) is an effective means to solve the above problems. The loss of epithelial barrier function in inflamed colonic tissues of UC patients leads to increased mucosal permeability, which allows the NDDS to passively target the inflamed colonic tissue through an epithelial-enhanced permeability and retention (eEPR) effect.…”

Section: Introductionmentioning

confidence: 99%

Mucoadhesive Nanoparticles Enhance the Therapeutic Effect of Dexamethasone on Experimental Ulcerative Colitis by the Local Administration as an Enema

Dong

Deng

et al. 2023

DDDT

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Background As the first-line drug to treat ulcerative colitis (UC), long-term use of glucocorticoids (GCs) produces severe toxic and side effects. Local administration as enema can increase the local GCs concentrations and reduce systemic exposure to high oral doses by directly delivering GCs to the inflammation site in the distal colorectum. However, UC patients are often accompanied by diarrhea, leading to the short colonic residence time of GCs and failure to exert their function fully. Purpose A kind of mucoadhesive nanoparticles (NPs) loading different dexamethasone derivatives (DDs) were developed, which could attach to the positively charged inflammatory colonic mucosa through electrostatic adsorption after administered by enema, thereby improving the local concentration and achieving effective targeted therapy for UC. Methods Two DDs, dexamethasone hemisuccinate and dexamethasone phosphate, were synthesized. In NPs preparation, The core PEI-DDs NPs were built by the electrostatic adsorption of DDs and the cationic polymer polyethyleneimine (PEI). Then, the natural polyanionic polysaccharide sodium alginate (SA) was electronically coated around NPs to construct the final SA-PEI-DDs NPs, followed by the in vitro stability and release tests, in vitro and in vivo colonic mucosal adhesion tests. In the in vivo anti-UC test, the experimental colitis mice were induced by 2,4,6-trinitrobenzenesulfonic acid. The body weight and disease activity index changes were measured, and the myeloperoxidase activity, pro-inflammatory cytokines concentration, and hematoxylin and eosin staining were also investigated to evaluate the therapeutic effect of NPs. Results The structures of two DDs were demonstrated by 1 H-NMR and MS. Both NPs were negatively charged and achieved high loading efficiency of DDs, while their particle sizes were significantly different. NPs showed good stability and sustained release properties in the simulated colonic environment. Moreover, the negative charge on the of NPs surface made them easier to adhere to the positively charged inflammatory colonic mucosa, thereby enhancing the enrichment and retention of DDS in the colitis site. Furthermore, the NPs exhibited better therapeutic effects than free Dex on the experimental colitis mice induced by TNBS through the enema rectal. Conclusion These results indicated the mucoadhesive NPs as a kind of novel nano-enema showed great potential to achieve efficient treatment on UC.

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Section: Introductionmentioning

confidence: 99%