Ulcer Prevention Effect Of 3,4,5-Tihydroxy-N0-[(2-Methyl-1H-Indol-3yl)Methylidene]Benzohydrazide In HCl/Ethanol-Induced Gastric Mucosal Damage In Rats

Abstract

The newly synthesized, 3,4,5-Trihydroxy-N 0-[(2-methyl-1H-indol-3-yl)-methylidene] benzohydrazide (TIBH), is an indole and gallic acid derivative. The aim of this research investigation was to evaluate the acute toxicity and the ulcer prevention potential of TIBH in HCl/Ethanol-induced gastric ulcer rat model. Six groups of rats were orally received 5ml/kg of vehicle (1 % Carboxy methyl cellulose) for the normal and ulcer control groups each, Omeprazole (20mg/kg) for positive control, 50 mg/kg, 100 mg/kg and 200 mg/kg of TIBH for experimental groups, respectively. After one hour, instead of rats in the normal group which received 5ml/kg of 1% CMC, other groups received 5ml/kg of HCl/Ethanol. All rats were sacrificed after one additional hour. Gastric juice, gastric mucosa, morphologies of gastric ulcers and protein expressions of both control and treatment groups were evaluated. TIBH showed a ulcer prevention potential by increase of the mucus secretion, decrease of the gastric acidity, up-regulation of HSP70 protein, down-regulation of Bax protein, decrease of the lipid peroxidation and the increase of the Superoxide dismutase (SOD) activity in gastric tissue homogenate. Acute toxicity assay exposed valuable information on the safety of this compound. TIBH had a dose dependent ulcer prevention potential against HCl/Ethanol-triggered gastric ulcer.

Protective effect of silkworm pupa oil on hydrochloric acid/ethanol‐induced gastric ulcers

“…A low degree of cell necrosis in the glandular cavity was observed, and inflammation and lymphatic dissolution decreased. The preventative effect of silkworm pupa oil in the HSPO group was thus better than that in the HSPO group …”

“…The preventative effect of silkworm pupa oil in the HSPO group was thus better than that in the HSPO group. [21][22][23] Gastric secretion volume and gastric juice pH in mice Table 4 shows that the mice with gastric ulcers in the control group secreted the highest amount of gastric juice, whereas the pH of gastric juice was lowest, in contrast to the normal group. The experimental group using pupa oil showed a reduction in the gastric juice secretion and an increase in gastric juice pH.…”

Gastroprotective Effect of Sinapic Acid on Ethanol-Induced Gastric Ulcers in Rats: Involvement of Nrf2/HO-1 and NF-κB Signaling and Antiapoptotic Role

“…Therefore, these molecules and pathways exhibited a protective role against gastric ulceration induced by ethanol and other insults. Furthermore, ROS/RNS increase oxidative-stress-induced apoptosis in gastric mucosal cells ( Tayeby et al, 2017 ). Moreover, ethanol promotes hypersecretion of gastric acid, proinflammatory cytokines, and ROS/RNS, which together function to induce apoptosis and suppress the production of NO and prostaglandin E2 ( Laloo et al, 2013 ; Antonisamy et al, 2014 ; Albaayit et al, 2016 ).…”

Gastro protecting influence of Topiramate in ethanol produced gastric ulcers in rats

“…Regular alcohol use damages gastric mucosa and is associated with gastric inflammation, ulceration, and even carcinoma of the gastric tissue [ 2 ]. Alcohol causes gastric damage through different mechanisms such as increased gastric secretion, decreased mucosal protection, the release of pro-inflammatory cytokines [ [3] , [4] , [5] , [6] ], and invasion of activated neutrophils, apoptosis, and oxidative stress [ 7 , 8 ]. On the other hand, different drugs, such as TPM, are effective against alcohol abuse and dependency.…”