UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription

Turner, Declan L.; Fritzlar, Svenja; Sadeghipour, Sara; Barugahare, Adele; Russ, Brendan E.; Turner, Stephen J.; Mathias, Rommel A.

doi:10.1016/j.isci.2022.105168

Cited by 2 publications

(5 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deletion of both proteins abrogated genome cleavage and Frontiers in Cell and Developmental Biology frontiersin.org led to accumulation of B-capsids, but no A-or C-capsids, suggesting potential structural determinants of terminase engagement (DeRussy and Tandon, 2015;Borst et al, 2016). Finally, UL34 deletion reduces viral titres by 100-fold and abrogates capsid maturation analogous to terminase mutants (Turner et al, 2022a). The precise function of UL34 is not yet clear, however RC and chromatin organisation may be involved (Turner et al, 2022a).…”

Section: Stage 7: Capsid Assembly and Genome Packagingmentioning

confidence: 99%

“…Finally, UL34 deletion reduces viral titres by 100-fold and abrogates capsid maturation analogous to terminase mutants (Turner et al, 2022a). The precise function of UL34 is not yet clear, however RC and chromatin organisation may be involved (Turner et al, 2022a).…”

Section: Stage 7: Capsid Assembly and Genome Packagingmentioning

confidence: 99%

“…This is strong evidence that DNA itself contributes to the physical properties of the RC. However, this is not the sole determinant of RC morphology at late times after DNA replication, as deletion of the RC resident protein UL34 had no impact on the levels of viral DNA, but altered the size, morphology, and electron density of RCs (Turner et al, 2022a). Further, fluorescent labelling of viral genomes showed colocalization with maturing capsids at the RC periphery, and potentially indicates that genome replication (Stage 4) and packaging (Stage 7) is coupled, at least at late times (Mariamé et al, 2018).…”

Section: Stage 2: Nuclear Trafficking and Early Viral Gene Expressionmentioning

confidence: 99%

“…Further, fluorescent labelling of viral genomes showed colocalization with maturing capsids at the RC periphery, and potentially indicates that genome replication (Stage 4) and packaging (Stage 7) is coupled, at least at late times (Mariamé et al, 2018). Interestingly, maturing capsids also mis-localise when RC residents UL34 and UL84 are deleted (Strang Blair et al, 2012;Turner et al, 2022a), further indicating that the RC periphery is involved in capsid maturation and genome packaging, although precise mechanisms currently remain elusive.…”

Section: Stage 2: Nuclear Trafficking and Early Viral Gene Expressionmentioning

confidence: 99%

See 3 more Smart Citations

The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction

Turner

Mathias²

2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that can cause severe disease in immunocompromised individuals, transplant recipients, and to the developing foetus during pregnancy. There is no protective vaccine currently available, and with only a limited number of antiviral drug options, resistant strains are constantly emerging. Successful completion of HCMV replication is an elegant feat from a molecular perspective, with both host and viral processes required at various stages. Remarkably, HCMV and other herpesviruses have protracted replication cycles, large genomes, complex virion structure and complicated nuclear and cytoplasmic replication events. In this review, we outline the 10 essential stages the virus must navigate to successfully complete replication. As each individual event along the replication continuum poses as a potential barrier for restriction, these essential checkpoints represent potential targets for antiviral development.

show abstract

Section: Stage 7: Capsid Assembly and Genome Packagingmentioning

confidence: 99%

Section: Stage 7: Capsid Assembly and Genome Packagingmentioning

confidence: 99%

Section: Stage 2: Nuclear Trafficking and Early Viral Gene Expressionmentioning

confidence: 99%

Section: Stage 2: Nuclear Trafficking and Early Viral Gene Expressionmentioning

confidence: 99%

See 2 more Smart Citations

The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction

Turner

Mathias²

2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Homologue of RNR R1, inhibits NF-κB signaling [153]; Deubiquitin protease [159]; Regulates the localization of pUL47 in vAC and promotes capsid maturation [84,157]; Releases viral DNA from capsid [158]; Contributes to vAC biogenesis [160]; Contributes to viral growth, virion stability and virus entry [161] Immune evasion [153] UL48A (UL48.5) Smallest capsid protein [162]; Locates on tips of hexons in capsids [163] Unknown UL49 DNA replication [164][165][166]; Regulates late-stage gene transcription, vPIC member [167] Unknown Table 1. Cont.…”

Section: Ul48mentioning

confidence: 99%

Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review

Zeng

Cao

Yang

et al. 2023

Viruses

View full text Add to dashboard Cite

Human cytomegalovirus (HCMV) is a widespread pathogen that poses significant risks to immunocompromised individuals. Its genome spans over 230 kbp and potentially encodes over 200 open-reading frames. The HCMV transcriptome consists of various types of RNAs, including messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs), with emerging insights into their biological functions. HCMV mRNAs are involved in crucial viral processes, such as viral replication, transcription, and translation regulation, as well as immune modulation and other effects on host cells. Additionally, four lncRNAs (RNA1.2, RNA2.7, RNA4.9, and RNA5.0) have been identified in HCMV, which play important roles in lytic replication like bypassing acute antiviral responses, promoting cell movement and viral spread, and maintaining HCMV latency. CircRNAs have gained attention for their important and diverse biological functions, including association with different diseases, acting as microRNA sponges, regulating parental gene expression, and serving as translation templates. Remarkably, HCMV encodes miRNAs which play critical roles in silencing human genes and other functions. This review gives an overview of human cytomegalovirus and current research on the HCMV transcriptome during lytic and latent infection.

show abstract

UL49 is an essential subunit of the viral pre-initiation complex that regulates human cytomegalovirus gene transcription

Cited by 2 publications

References 61 publications

The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction

The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction

Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review

Contact Info

Product

Resources

About