UJI TOKSISITAS ORAL AKUT SINGLE DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

Fitria, Laksmindra

doi:10.31943/mangiferaedu.v4i1.39

Cited by 2 publications

(10 citation statements)

References 7 publications

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“…Dalam uji toksisitas juga dapat diperoleh berbagai temuan baru yang tidak terduga dari suatu bahan alam yang dapat bermanfaat untuk kesehatan. Hal ini dapat membuka peluang bahwa bahan yang diuji tersebut memiliki potensi positif terhadap sistem biologis tertentu (Fitria, et al, 2019).…”

Section: Pembahasanunclassified

“…Uji toksisitas oral akut dosis tunggal (single dose) buah luwingan oleh Fitria et al (2019) telah dilakukan dengan cara administrasi oral filtrat buah luwingan muda dan matang konsentrasi 100 % sebanyak satu kali saja di awal percobaan, kemudian diamati efeknya hingga hari ke-14. Empat belas hari merupakan jangka waktu di mana suatu zat yang masuk ke dalam tubuh (xenobiotik) dimetabolisme hingga memunculkan efek terhadap kondisi fisiologis.…”

Section: Pembahasanunclassified

“…Bentuk dan cara pemberian buah luwingan pada uji toksisitas oral dalam penelitian ini sama dengan uji toksisitas oleh Fitria et al (2019) Mengenai bentuk sediaan buah luwingan yang diujicobakan, kami memilih bentuk filtrat dengan pertimbangan bahwa selama ini buah luwingan dikonsumsi oleh masyarakat secara langsung sebagai buah konsumsi maupun bahan dalam masakan sehari-hari. Jus atau filtrat merupakan bentuk yang paling alami dan paling mudah diadministrasikan pada hewan uji.…”

Section: Pembahasanunclassified

“…Berdasarkan temuan ini maka perlu dilakukan uji toksisitas yang sama dengan konsentrasi yang lebih rendah, serta uji toksisitas dengan periode yang lebih panjang (tahap subkronik dan kronik) guna mempelajari efek buah luwingan jika dikonsumsi terus menerus dalam jangka waktu panjang, Sementara itu, filtrat buah luwingan tidak menyebabkan perubahan profil leukosit (Gambar 6). Pada uji toksisits akut dosis tunggal (Fitria et al, 2019) dinyatakan bahwa hewan yang diberi filtrat buah luwingan muda ataupun matang mengalami peningkatan limfosit yang signifikan hingga di atas kisaran normal. Hal ini diduga karena administrasi dosis tunggal filtrat buah luwingan dianggap sebagai antigen yang harus dilawan.…”

Section: Pembahasanunclassified

“…Oleh karena itu perlu dilakukan penelitian untuk mempelajari keamanan dan efek sampingnya melalui serangkaian uji toksisitas oral (Walum, 1998). Uji toksisitas oral akut single dose filtrat buah luwingan telah dilakukan oleh Fitria et al (2019) menggunakan tikus Wistar sebagai model praklinis. Hasil menunjukkan no observed adverse effect level (NOAEL) pada konsentrasi 100 % yang berarti buah luwingan relatif aman dikonsumsi namun masih terbatas untuk periode yang singkat.…”

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UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

et al. 2020

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Hairy fig is a tropical medium-sized tree that produces abundant fruits throughout the year. In some Asian countries, the fruits are consumed as traditional medicine and food ingredient. Meanwhile in Indonesia there has not been much use. A series of oral toxicity tests must be conducted to study the possibility of toxic effects and the safety before further exploration. Oral single dose toxicity study of young and ripe hairy fig fruit filtrate has been carried out. Results demonstrated no observed adverse effect level (NOAEL) at a concentration of 100%. This study was aimed to continue the oral toxicity test with repeated dose following standard toxicity procedure by OECD Test Guideline No. 407 with some modifications. Parameters observed were mortality, sublethal effects consisted of physical conditions and behavior, body weight, core temperature, complete blood count, as well as liver, heart, and renal functions by measuring ALT, AST, and creatinine, respectively. Sampling points on day 0, 7, 14, 21, and 28. Results showed no observed adverse effect level (NOAEL) in both young and ripe fruits filtrate at a concentration of 100% however with a tendency to cause anemia and associate with renal dysfunction. Therefore, it is necessary to perform similar method of toxicity test but with lower concentration, also continue with further toxicity tests (subchronic and chronic periods).

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UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

et al. 2020

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Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

Fitria

Sari²,

Handayani

et al. 2022

Jurnal Biodjati

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Hairy fig (Ficus hispida) fruits (HFF) are widely consumed as food and traditional medicine in several West Asian countries, both the unripe fruit (UHFF) and ripe fruit (RHFF). However, they are not commonly utilized in Indonesia. Acute oral toxicity studies reported No Observed adverse effect level (NOAEL). Further, a reproductive toxicity study found that UHFF boosted spermatogenesis and increased the quality and quantity of spermatozoa. Meanwhile, RHFF exhibited the opposite effects. To provide comprehensive information from the previous study, this research was conducted to evaluate the hemotoxicity of UHFF and RHFF about their impact on the male reproductive system. Nine Wistar rats were assigned into three groups: the first group received UHFF juice, the second group received RHFF juice, and the third group received distilled water as control. The volume of each treatment was 2 mL/individual/day for 77 days. On days 0, 28, and 77, blood samples were collected for routine hematological profile examination using a hematology analyzer (Sysmex®XP-100). Data were analyzed using a one-way ANOVA test and Duncan's test (α=0.05) to discover significant differences between groups and times. Results showed that consuming hairy fig fruit, especially the UHFF, had an unfavorable effect on erythrocytes resulting in hypochromic microcytic anemia. Still, there was no adverse effect on leukocytes and platelets. Anemia may have occurred due to the presence of hemotoxic compounds that interfere with the synthesis and binding of hemoglobin or because the hairy fig fruit filtrates were oxidized, thereby increasing the level of oxidative stress within the body, of which is a drop in hemoglobin levels.

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UJI TOKSISITAS ORAL AKUT SINGLE DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

Cited by 2 publications

References 7 publications

UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769)

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