UJI DAYA HAMBAT DAUN, KULIT BATANG DAN BUAH SAWO MANILA MUDA (Manilkarazapota L)TERHADAP BAKTERI Escherichia coliDAN Staphylococcus aureus MENGGUNAKAN METODE DIFUSI SUMURAN

Primadiamanti, Annisa; Purnama, Robby Chandra; Aulia, Riza

doi:10.33024/jaf.v5i2.4086

Cited by 2 publications

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“…This research is in line with Hasanah (2018) and Tampubolon et al (2023) that it has not been able to inhibit the growth of E. coli bacteria. 8,40 However, research from Primadiamanti (2020) found that it could inhibit E. coli bacteria even though the results were intermediate. or the ability to inhibit it was moderate in the test bacteria.…”

Section: Resultsmentioning

confidence: 99%

Testing The Antimicrobial Effectiveness of Manila Sauce Leaves (Manilkara Zapota L) against Bacteria Escherichia Coli: In Vitro Study

Azizah,

Kartika,

Hasbi

et al. 2024

JLL

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Diarrhea remains a significant public health concern globally, particularly affecting children under five years old. Traditional herbal remedies, including Manila Sapodilla (Manilkara zapota L) leaves, have been utilized in Indonesia for treating diarrhea due to their perceived efficacy and safety. This in vitro study aimed to assess the antimicrobial effectiveness of Manila Sapodilla leaf extract against Escherichia coli bacteria, a common cause of diarrhea. True experimental design was employed, utilizing various concentrations (25%, 50%, 75%, and 100%) of Manila Sapodilla leaf extract. The extract was obtained through maceration using ethanol followed by dilution with distilled water. Antimicrobial activity was evaluated using the Kirby-Bauer diffusion method, measuring inhibition zones formed around Escherichia coli colonies. Results demonstrated that Manila Sapodilla leaf extract exhibited inhibitory effects against Escherichia coli at all tested concentrations, albeit with varying degrees of effectiveness. The inhibition zones ranged from 5.37 mm to 8.53 mm, indicating resistance. Positive control using Ciprofloxacin displayed a significantly larger inhibition zone (21.75 mm), indicating sensitivity. These findings suggest the potential of Manila Sapodilla leaf extract as an alternative treatment for Escherichia coli infections. However, further research, including phytochemical analysis and exploration of different extraction methods and concentrations, is warranted to elucidate its therapeutic potential fully. This study contributes to the ongoing search for alternative herbal-based antibiotics to combat bacterial infections effectively.

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Section: Resultsmentioning

confidence: 99%

Testing The Antimicrobial Effectiveness of Manila Sauce Leaves (Manilkara Zapota L) against Bacteria Escherichia Coli: In Vitro Study

Azizah,

Kartika,

Hasbi

et al. 2024

JLL

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“…Penelitian relevan [14], hasil ekstrak etanol buah sawo konsentrasi 20%, 40%, 60% dengan metode difusi sumuran, tidak menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 80% dan 100% menunjukkan hambatan dengan rerata 8,29 mm dan 9,43 mm.…”

Section: Gambar 1 Uji Daya Hambatunclassified

Uji Efektivitas Antibakteri Ekstrak Etanol Buah Sawo (Manilkara zapota L.) Terhadap Escherichia coli Metode Difusi

Endrawati,

Indrawati,

Parmadi

et al. 2023

JIPP

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Tanaman sawo merupakan tanaman buah yang berasal dari Guatemala (Amerika Tengah) Meksiko, dan Hindia Barat. Tanaman ini sudah menyebar keberbagai benua salah satunya Asia, yaitu Indonesia. Salah satu bagian dari tanaman sawo yang dapat dimanfaatkan sebagai bahan obat adalah buah nya. Buah sawo muda mengandung tanin dan flavonoid yang dapat berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol buah sawo efektif dalam menghambat pertumbuhan bakteri Escherichia coli dengan metode difusi pada konsentrasi tertentu. Penelitian bersifat eksperimental dengan membuat sumuran pada media yang sudah diinokulasi bakteri Escherichia coli selebar 9mm dan di isi dengan ekstrak etanol buah sawo dengan konsentrasi 12,5%; 25%; 50%, kontrol positif kloramfenikol 250 mg, kontrol negatif DMSO 10% sebanyak 250 µg. Zona hambat yang terbentuk di ukur menggunakan jangka sorong. Hasil perhitugan zona hambat di analisis menggunakan uji One Way Annova dengan taraf signifikansi 0,05. Ekstrak etanol buah sawo dapat menghambat pertumbuha bakteri pada konsentrasi 12,5% dengan rerata hambatan sebesar 3,67 mm; konsentrasi 25% mengahambat dengan rerata 5,33 mm; konsentrasi 50% menghambat dengan rerata diamter 8,3 mm; kloramfenikol memberi hambatan dengan rerata diameter 29 mm; sedangkan DMSO tidak memeberi hambatan pada pertumbuhan bakteri Escherichia coli. Ekstrak etanol buah sawo dapat menghambat bakteri Escherichia coli pada semua konsentrasi.

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UJI DAYA HAMBAT DAUN, KULIT BATANG DAN BUAH SAWO MANILA MUDA (Manilkarazapota L)TERHADAP BAKTERI Escherichia coliDAN Staphylococcus aureus MENGGUNAKAN METODE DIFUSI SUMURAN

Cited by 2 publications

References 0 publications

Testing The Antimicrobial Effectiveness of Manila Sauce Leaves (Manilkara Zapota L) against Bacteria Escherichia Coli: In Vitro Study

Testing The Antimicrobial Effectiveness of Manila Sauce Leaves (Manilkara Zapota L) against Bacteria Escherichia Coli: In Vitro Study

Uji Efektivitas Antibakteri Ekstrak Etanol Buah Sawo (Manilkara zapota L.) Terhadap Escherichia coli Metode Difusi

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