Uji Beberapa Konsentrasi Bakteri Bacillus sp. Endofit untuk Meningkatkan Pertumbuhan Bibit Kakao (Theobroma cacao L.)

Abstract

Development of cocoa (Theobroma cacao L.) requires high-quality seeds. Adding growth substance from Bacillus sp. endophytes is expected to increase the growth of cocoa seedlings. The purpose of this research was to evaluate the ability of Bacillus sp. endophytes to produce Indole Acetic Acid (IAA) hormone, evaluate the effects of Bacillus sp. endophytes application on growth of cocoa seedling, and obtain the best concentration to increase growth of cocoa seedling. The experiments were conducted in the laboratory and in the field from June to October 2015. The study was conducted in two stages: in vitro and in vivo.The first experiment tested the ability of Bacillus sp. endophytes to produce IAA using tryptophan enriched picovskaya liquid medium and non-enriched tryptophan. The results from the first experiment showed that all Bacillus sp. endophytes produce IAA hormones. The second experiment tested the concentrations of Bacillus sp. endophytes to improve the growth of cocoa seedlings. The experiment was conducted using a completely randomized design consisting of 5 treatments and 4 replications, i.e., without treatment of Bacillus sp., and concentrations of 1011, 1012, 1013, and 1014 cfu/mL. The parameters observed were the number of bacterial colonies of Bacillus sp. endophytes, cocoa seed height, stem diameter, number of leaves and planting area of 4 month cocoa seedlings. The results from the second experiment showed that all concentrations of Bacillus sp. endophytes increase the growth of cocoa seedlings. The concentration of 1011 cfu/mL produced more colony in planting medium, increased height, stem diameter, leaf number and leaf area in cocoa seedlings.

Uji kemampuan bakteri diazotrof asal perakaran bawang merah dalam mendukung pertumbuhan dan hasil tanaman bawang merah

“…Peningkatan bobot kering akar diduga disebabkan oleh hormon yang dihasilkan bakteri mampu merangsang pertumbuhan. Hal ini sejalan dengan hasil percobaan Puspita et al, (2018) Nitrogen dalam biosintessi klorofil (Halmedan et al, 2017;Sari et al, 2016).…”

Pemanfaatan Mikroba Tanah dalam Pembuatan Pupuk Organik serta Peranannya terhadap Tanah Aluvial dan Pertumbuhan Bibit Tanaman Kakao

“…IAA yang dapat membantu pertumbuhan tanaman. Puspita et al (2018) Pemberian Bacillus sp. Endofit konsentrasi 1011 cfu/mL mampu meningkatkan pertumbuhan bibit kakao melalui pertambahan tinggi, diameter batang, jumlah daun, luas daun dan jumlah koloni yan lebih baik.…”

Induced resistance by Bacillus subtilis on oil palm seedling infected by Ganoderma boninense

“…It is presumed that the growth of the oil palm seedlings is slower in responding to its reaction. In the experiment done by Puspita et al (2018), the same application on cocoa seedlings shows different results i.e. the endophytic Bacillus from the roots, stems, bunch, and the leaf tissues contain IAA hormones with a concentration interval of 118-343 ppm which can increase the growth of cocoa seedlings.…”

“…Puspita et al (2013) state that the produced IAA functions as the plant growthpromoter by stimulating cell division, cell enlargement and optimal absorption of water and nutrients that affect plant growth. The result shows that endophytic Bacillus of the leaves at a density of 10 11 cfu mL -1 could increase the seedling height, the number of leaves and the area of cocoa leaf (Puspita et al 2018). The Bacillus species used as a biological agent may bring a direct impact on the plants grow through the growth hormone synthesis (Amer and Utkhede 2007).…”