Uji Aktivitas Antioksidan Kombinasi Ekstrak Daun Kersen (Muntingia calabura Linn.) dan Daun Sirsak (Anonna muricata Linn.) Metode DPPH (2,2-diphenyl-1- picrilhidrazyl)

Harningsih, Tri; Wimpy, Wimpy

doi:10.31001/biomedika.v11i2.422

Cited by 4 publications

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“…3 di atas didapatkan nilai IC50 ekstrak daun beluntas dan sirsak tunggal adalah 58 ppm dan 51 ppm. Berbeda dengan hasil penelitian Harningsih, [17] aktivitas antioksidan ekstrak daun sirsak didapatkan nilai IC50 sebesar 116,537 ppm dan dari Wanita [9] diperoleh IC50 dari daun beluntas sebesar 37,25 ppm. Perbedaan nilai IC50 dari penelitian sebelumnya disebabkan karena beberapa faktor, diantaranya tingkat kesuburan tanah, iklim, geografis yang akan mempengaruhi kandungan bioaktif dalam tanaman [18].…”

Section: Uji Antioksidan Menggunakan Metode Dpphunclassified

Aktivitas Aantioksidan Kombinasi Daun Sirsak (Anonna Muricata L.) dan Daun Beluntas (Pluchea Indica L.)

Hasanah,

Inayah

2023

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Soursop (Anonna Muricata L.) and Beluntas (Pluchea indica L.) are plants that contain secondary metabolites that have potential as antioxidants. The purpose of this study was to determine the activity of soursop leaf samples, beluntas and their combination in reducing DPPH. Samples were made into powder followed by maceration and evaporated the solvent to become an extract. Antioxidant activity testing with DPPH silencing method with UV-Vis spectrophotometer. Antioxidant activity test results for soursop leaf samples, beluntas, combinations (1:1), (1:2) and (2:1) have IC50 values of 58, 51, 57, 61 and 68 ppm, respectively. Further analysis Combination index of the combination (1:1) has a synergistic effect and the combination (1:2) and (2:1) has an antagonistic effect. Keywords: Antioxidant Activity, Anonna Muricata L. leaf, Pluchea indica L. leaf, DPPH

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Section: Uji Antioksidan Menggunakan Metode Dpphunclassified

Aktivitas Aantioksidan Kombinasi Daun Sirsak (Anonna Muricata L.) dan Daun Beluntas (Pluchea Indica L.)

Hasanah,

Inayah

2023

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“…Adanya senyawa dalam tabir surya dapat menyerap sinar UV pada panjang gelombang 290 nm hingga 450 nm (5) . Antioksidan merupakan senyawa yang dapat meredam kerja radikal bebas dan mengubahnya menjadi senyawa non radikal (6) .…”

Section: Pendahuluanunclassified

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Wimpy¹,

Harningsih²,

Larassati³

2020

j. ilm. manuntung sains farm. kesehat.

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The kepok banana peel extract (Musa paradisiaca Linn) and avocado peel extract (Persea americana Mill) contain alkaloids, flavonoids, saponins and steroids. There is a linear relationship between antioxidant activity and sunscreen capability. The stronger antioxidant activity,the better sunscreen capability will be. This study aims to determine antioxidant activity and sunscreen capability from the combination of kepok banana peel extract and avocado peel extract compared to it's single form. This study was conducted with quota sampling. The preparation of ethanol extract from kepok banana peel and avocado peel were using maceration method with 96 % of ethanol. The study was started by collecting samples, making the extract with 96% ethanol solvent, then continue with phytochemical testing, antioxidant activity testing of kepok banana peel extract and avocado peel extract with variation ratio (1:0), (1:1), (1:2), (2:1), (0:1). Further tests including cream formulation, cream stability test and in vitro SPF value determination was also conducted. The result of IC50 determination from : kepok banana peel extract is 122,77, belongs to medium category; avocado peel extract is 11,50, belongs to very strong category; (kepok banana peel extract : avocado peel extract) (1:1) is 39,88, belongs to very strong category; (kepok banana peel extract : avocado peel extract) (1:2) : 25,31, belongs to very strong category; (kepok banana peel extract : avocado peel extract) (2:1) : 76,77, belongs to the strong category. The result of SPF measurement from the single form kepok banana is 16,21, belongs to ultra category; single form avocado peel is 21,21, belongs to ultra category; (kepok banana peel extract : avocado peel extract) (1:1) is 21,32, belongs to ultra category; (kepok banana peel extract : avocado peel extract) (1:2) is 21,52 belongs to ultra category; (kepok banana peel extract : avocado peel extract) (2:1) is 21,51, belongs to ultra category. The antioxidant activity from the variation ratio of banana peel extract and avocado peel extract (1:2) is stronger than the single form of banana peel extract, but it is weaker than the single form of avocado peel extract. The combination of kepok banana peel extract and avocado peel extract (1:2) has better sunscreen capability than it's single form.

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“…It can be seen from the IC50 value of guava leaves that was lower than the IC50 of the combination of guava leaves and Meniran plant. The lower the IC50 value is, the stronger antioxidant activity will be(Harningsih & Wimpy, 2018) The results of the analysis also depict the differences in the IC50 values in all combinations. The combination of Meniran plants and guava leaves with the ratio of 1: 2 had the strongest IC50 value when compared with the singular form and the other combinations because the guava leaves had more phytochemical components such as triterpenoids(Arya et al, 2012) than Meniran plants.…”

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confidence: 96%

The Screening of Antioxidant Activities of Meniran Plants (Phyllanthus niruri Linn) and Guava Leaves (Psidium guajava Linn) Combination Using DPPH Free Radical Method

Wimpy¹,

Harningsih²,

Sisvitara³

2021

biomedika

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Degenerative disease is a serious health problem and causes a lot of deaths in Indonesia. One of the most dangerous degenerative diseases is cancer. Free radicals play a role in oxidative stress in the later stages of carcinogenesis. Antioxidant delays or inhibits cellular damage mainly through its free radical scavenging property. Meniran plants and guava leaves have high radical-scavenging activities. The previous studies reported that the combination of ginger and Meniran plant extract has a stronger antioxidant activity than the extract of a single plant. This research aims at determining the antioxidant activities of Meniran plants and guava leaves compared with its singular form. This research was conducted from October to December 2016 at the Chemical Laboratory of Nasional Health Science Institute and the Center for Development and Research of Medicinal Plants and Traditional Medicine, Tawangmangu, Karanganyar. The antioxidant activity assay was done using DPPH free radical method and vitamin C was used as the control. They were measured with UV-Vis Spectrophotometer. This study concludes that the IC50 value of Meniran plants was 30.689 ppm and the IC50 value of guava leaves was 13.7859. The IC50 values of Meniran plant and guava leaf combination with various ratios were 20.6095 ppm (1:1), 12.5629 ppm (1:2), and 16.841 ppm (2:1). The combination of Meniran plant and guava leaf extract (1: 2) had the strongest antioxidant activity of 12.56 ppm.

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Uji Aktivitas Antioksidan Kombinasi Ekstrak Daun Kersen (Muntingia calabura Linn.) dan Daun Sirsak (Anonna muricata Linn.) Metode DPPH (2,2-diphenyl-1- picrilhidrazyl)

Cited by 4 publications

References 0 publications

Aktivitas Aantioksidan Kombinasi Daun Sirsak (Anonna Muricata L.) dan Daun Beluntas (Pluchea Indica L.)

Aktivitas Aantioksidan Kombinasi Daun Sirsak (Anonna Muricata L.) dan Daun Beluntas (Pluchea Indica L.)

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The Screening of Antioxidant Activities of Meniran Plants (Phyllanthus niruri Linn) and Guava Leaves (Psidium guajava Linn) Combination Using DPPH Free Radical Method

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