UJI AKTIVITAS ANTIKANKER PAYUDARA SENYAWA ANDROGRAFOLIDA DARI TUMBUHAN SAMBILOTO (Andrographis paniculata (Burm F) Ness.) TERHADAP HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 (HER-2) SECARA IN SILICO

Suherlan, Sintya; Rohayah, Rohayah; Fakih, Taufik Muhammad

doi:10.29313/jiff.v4i2.7784

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Kanker payudara adalah sel jaringan pada payudara yang mengalami pertumbuhan abnormal, dimana sel dalam kelenjar payudara mengalami tumbuh kembang yang tidak terkon-Penulis koresponden: Alma Yunita Sari, alma19003@mail.unpad.ac.id trol (Suherlan, et al, 2021). Kanker payudara belum diketahui penyebabnya secara pasti, namun kemungkinan faktor risiko payudara berkaitan baik secara langsung maupun tidak langsung dengan kadar hormon estrogen dalam tubuh ataupun estrogen yang tidak diimbangi progesteron.…”

Section: Pendahuluanunclassified

Potensi Senyawa Aktif Tanaman Herbal untuk Pengobatan Kanker Payudara dengan Metode Penambatan Molekuler: Review Artikel

Yunita Sari,

Febrina

2023

jfu

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Breast cancer is one of the leading causes of death in women worldwide. Breast cancer can be triggered due to physical, chemical, environmental factors and can be passed down from generation to generation through genetics. Therefore, recently, studies have been intensively conducted on the use of medicinal plants for the treatment of breast cancer as an alternative because they have minimal side effects compared to other chemotherapy. This review aims to describe medicinal plants and their active compounds that have the potential as anticancer therapy which has been carried out in silico studies with molecular anchoring methods. The method used in writing this article is a literature review both nationally and internationally over the last five years using the keywords breast cancer, molecular docking, herbal plants, anticancer, in databases such as PubMed, Cochrane, and Google Scholar. Herbal plants in in silico research that have high potential for further research as cancer therapy include 33 herbal plants with different active compounds and protein targets.

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Section: Pendahuluanunclassified

Potensi Senyawa Aktif Tanaman Herbal untuk Pengobatan Kanker Payudara dengan Metode Penambatan Molekuler: Review Artikel

Yunita Sari,

Febrina

2023

jfu

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“…Protein HER-2 menjadi target pengobatan kanker payudara dan sebagai prediktor respon kemoterapi kanker payudara (Widjaja, 2011). Penelitian in silico yang dilakukan oleh Suherlan et al,(2021), menyebutkan bahwa senyawa andrografolida tumbuhan sambiloto dapat dijadikan senyawa kandidat antikanker payudara berdasarkan hasil penambatan molekul dengan reseptor HER-2. Gambar 2.…”

Section: Protein Dockingunclassified

Interaksi Senyawa Taxifolin dari Buah Kasturi (Mangifera casturi) sebagai Antikanker Payudara: Evaluasi Docking Molekular

Adawiyah

Komari

2021

J Nat Sci

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Taxifolin also called dihydroquercetin is a flavonoid. Taxifolin is included in the flavonol subclass and polyphenol class. Taxifolin has been found in the leaves, bark and flesh of Mangifera casturi. Taxifolin is thought to have potential as an antibreast cancer through its activity as an anti-neoplastic agent. The occurrence of breast cancer begins with overexpression of the Human Epidermal Growth Factor Receptor 2 (HER-2) protein which can induce dimerization and autophosphorylation of the tyrosine kinase enzyme, resulting in migration and metastasis of breast cancer cells. This study aims to determine the interaction of taxifolin with HER-2 protein by molecular docking. Molecular docking using the SwissDock web server. Parameters of molecular docking include Gibbs free energy and interaction of taxifolin ligands and HER-2 residues. The results of docking molecular expressed by free energy Gibbs of taxifolin compounds with HER-2 protein is -7,99 kcal / mol, while the free energy Gibbs of the native ligand bond with HER-2 protein is -10.76 kcal / mol. The residues on HER-2 that interacts with taxifolin are GLY727, GLY804, LEU726, THR798, ASP863, ALA730, SER728, ARG849, GLY729, CYS805, THR862, VAL734, LEU852, LYS753, ALA751, ASP808, ARG849, ASN850. The value of Gibbs free energy and the interaction of residues indicate that taxifolin compounds have potential as anticancer breasts. Taxifolin as an inhibitor of HER-2 protein expression.

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“…This method is beneficial for protein modeling and interactions between the examined protein and the chemicals used to represent it [10]. Employed molecular docking techniques to assess the affinity and interaction between ligands and EGF receptors [11].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Genes Encoding for Human Epidermal Growth Factor (hEGF) from Human Blood: In Vitro and In Silico Study

2023

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The 6.12 kDa human epidermal growth factor (hEGF) protein is expressed in the body and plays a role in cell proliferation and differentiation. This research aims to isolate the hEGF genes, determine the phylogenetic relationship between organisms based on the hEGF gene, construct the 3D structure of the hEGF protein, and identify the precise binding position between the hEGF protein and the tested Myosin-9. DNA was isolated from blood of healthy individuals followed by amplification using specified primers. DNA sequencing was utilized to identify the amplification results before generating the phylogenetic trees. The retrieved sequences were then modeled using the Swiss model and docking proteins in silico. In three samples, DNA was successfully amplified to 700 bp and the phylogenetic analysis revealed that the three hEGF gene samples belonged to the same clades. With a 30 % identity seq, aligned result sequences modeled using the Swiss model created proteins which were not homologous. The outcomes of this modeling were compared to the hEGF protein from the database, which had a sequence identity of greater than 90 %. The i-RMSD value of the target protein (5.4 +/- 0.4), the Van der Waals energy (-29.3 +/- 1.7), and the Z-score were determined by simulation (-1.7). These results indicate a potential interaction between the isolated EGF protein and the myosin-9 tested. HIGHLIGHTS Human Epidermal Growth Factor (hEGF) protein is a potential protein expressed in the body and plays a role in cell proliferation and differentiation Isolation of the EGF gene through human blood can be carried out to get data on protein size, phylogenetic analysis, and protein structure The results of in vitro study were modeled in silico to obtain the three-dimensional structure as well as intermolecular interactions of the protein Information beginning this study is used in follow-up research to predict EGF communications with other molecules GRAPHICAL ABSTRACT

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UJI AKTIVITAS ANTIKANKER PAYUDARA SENYAWA ANDROGRAFOLIDA DARI TUMBUHAN SAMBILOTO (Andrographis paniculata (Burm F) Ness.) TERHADAP HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 (HER-2) SECARA IN SILICO

Cited by 3 publications

References 14 publications

Potensi Senyawa Aktif Tanaman Herbal untuk Pengobatan Kanker Payudara dengan Metode Penambatan Molekuler: Review Artikel

Potensi Senyawa Aktif Tanaman Herbal untuk Pengobatan Kanker Payudara dengan Metode Penambatan Molekuler: Review Artikel

Interaksi Senyawa Taxifolin dari Buah Kasturi (Mangifera casturi) sebagai Antikanker Payudara: Evaluasi Docking Molekular

Isolation of Genes Encoding for Human Epidermal Growth Factor (hEGF) from Human Blood: In Vitro and In Silico Study

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