UHPLC: The Greening Face of Liquid Chromatography

Abstract

Pharmaceutical analysis based on chromatographic separation is an important part of studies aimed at developing routine quality analysis of drugs. High-performance liquid chromatography (HPLC) is one of the main analytical techniques recommended for drug analysis. Although it meets many criteria vital for analysis, it is time-consuming and uses a relatively high amount of organic solvents compared to other analytical techniques. Recently, Ultra-high-performance liquid chromatography (UHPLC) has been frequently proposed as an alternative to HPLC, which means introducing an environment-friendly approach to drug analysis achieved by reducing the consumption of solvents. It also offers greater chromatographic resolution and higher sensitivity as well as requiring less time due to faster analysis. This review focuses on the basics of UHPLC, compares that technique with HPLC and discusses the possibilities of applying UHPLC for the analysis of different pharmaceuticals and biopharmaceuticals.

Analytical Methods to Characterize and Purify Polymeric Nanoparticles

“…This reduction on the filling particle size enables a finer separation of similar compounds. In addition, the working pressure of UHPLC equipment is markedly higher than that supported by conventional HPLC, which enables more rapid flow rates, resulting in shorter elution times and decrease on the solvent amount used [20,21].…”

Kinetics of Degradation of Biapenem

“…In dry air, in the range 363-393 K, temperature affected the reaction to a lesser extent than in an environment of increased relative air humidity (RH > 50%) (Table III). Compared to other carbapenems, the stability of biapenem in the solid state is greater as it shows lower susceptibility to degradation on exposure to increased relative air humidity [5,[14][15][16].…”

Comparative study of phenolic profile of fruit and juice samples of a progeny of ‘Meana’ × ‘Florina’ from an Asturian cider apple breeding program

“…Compared with HPLC, UHPLC offers greater chromatographic resolution and higher sensitivity, as well as requiring less time due to faster analysis. As a matter of fact, it constitutes an environment-friendly approach to food analysis achieved by reducing the consumption of solvents [17]. So far, this separation technique has been already applied to the determination of 21 major polyphenols in cider and dessert apples [9].…”