UHPLC-QqQ-MS/MS method development and validation with statistical analysis: Determination of raspberry ketone metabolites in mice plasma and brain

Yuan, Bo; Zhao, Danyue; Kshatriya, Dushyant; Bello, Nicholas T.; Simon, James E.; Wu, Qingli

doi:10.1016/j.jchromb.2020.122146

Cited by 12 publications

(13 citation statements)

References 23 publications

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“…The results from this study found that high-fat diet-induced obese mice have a higher bioavailability and absorption of RK, and RK also accumulated in lipid-rich tissues such as the brain. The results from the in vitro and mouse studies strengthen the claims of RK being a potential anti-obesity agent [ 57 ]. A single clinical study showed that a multi-ingredient supplement containing RK was able to reduce body weight, metabolic lipid, and inflammatory parameters in 45 obese individuals after an 8 week exercise and weight loss program.…”

Section: Potential Role Of Rk As An Anti-obesity Agentmentioning

confidence: 56%

“…A very recent publication describes the development and validation of ultra-high-performance liquid chromatography with a triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS) method for targeted determination of RK and its metabolites in plasma and brain specimens in mice. They believe that based on this method, it would be possible to study the pharmacokinetics and toxicity of RK as well as establish the safest effective dose that can be consumed for human use [ 57 ]. A pioneering study to assess the safety and toxicity implications of consuming RK as a dietary supplement generated a quantitative structure–activity relationship (QSAR) model that predicts the activity of new chemicals.…”

Section: Pharmacokinetic (Adme) and Safety Profile Of Rkmentioning

confidence: 99%

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Pharmacological Exploration of Phenolic Compound: Raspberry Ketone—Update 2020

Rao

Kurakula

Mamidipalli³

et al. 2021

Plants

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Raspberry ketone (RK) is an aromatic phenolic compound naturally occurring in red raspberries, kiwifruit, peaches, and apples and reported for its potential therapeutic and nutraceutical properties. Studies in cells and rodents have suggested an important role for RK in hepatic/cardio/gastric protection and as an anti-hyperlipidemic, anti-obesity, depigmentation, and sexual maturation agent. Raspberry ketone-mediated activation of peroxisome proliferator-activated receptor-α (PPAR-α) stands out as one of its main modes of action. Although rodent studies have demonstrated the efficacious effects of RK, its mechanism remains largely unknown. In spite of a lack of reliable human research, RK is marketed as a health supplement, at very high doses. In this review, we provide a compilation of scientific research that has been conducted so far, assessing the therapeutic properties of RK in several disease conditions as well as inspiring future research before RK can be considered safe and efficacious with limited side effects as an alternative to modern medicines in the treatment of major lifestyle-based diseases.

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Section: Potential Role Of Rk As An Anti-obesity Agentmentioning

confidence: 56%

Section: Pharmacokinetic (Adme) and Safety Profile Of Rkmentioning

confidence: 99%

Pharmacological Exploration of Phenolic Compound: Raspberry Ketone—Update 2020

Rao

Kurakula

Mamidipalli³

et al. 2021

Plants

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“…In this type of experiment, the “source” of a mass spectrometer typically refers to the electrospray ionization (ESI) chamber, the transmission capillary and other spaces maintained under intermediate vacuum by the rough pump prior to the internal high vacuum. While ESI is generally considered as a “soft” ionization technique, ions with weak bonds can still undergo fragmentation during ionization and desorption from the aerosol in the ESI chamber, with the fragmentation intensity affected by the mobile phase and the ESI gas and voltage settings 16–18 . At the end of the transmission capillary, a fragmentor voltage is applied to propel ion transmission, and additional fragmentation could happen due to collision between accelerated ions and the surrounding neutral gas (this is also known as the in‐source collision‐induced dissociation (CID), which is distinguished from the CID occurring in the designated collision cell by collision with an auxiliary gas such as nitrogen, helium or argon) 19,20 .…”

Section: Resultsmentioning

confidence: 99%

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

Lyu

Yuan

Dinssa³

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale The safe consumption of Solanum scabrum and S. nigrum berries (SNBs) depends on a reliable and rapid chemical screen for the testing of the fruit and/or final food and industrial products for the presence and level of toxic glycoalkaloids. Such a rapid and sensitive screen could also be used by those involved in food safety and forensics, industry, research labs and those in agriculture production, breeding and food processing. Significant variation in the content and composition of glycoalkaloids across SNBs has been reported. To facilitate high‐throughput targeted analysis, this work overcame the slow scan speed of a traditional triple quadruple mass spectrometry (QqQ) method by development of a pseudo‐MS3 method. Methods In‐source fragmentation functioned as a pseudo‐MS or pseudo‐hydrolysis to trim down the structurally diverse and complex glycosides into five types of aglycone ions, which were then analyzed using multiple reaction monitoring (MRM). Characteristic product ions were selected based on the aglycone skeleton and substitution pattern and associated fragmentation pathway. Results A compact method with only 15 MRM transitions were developed for high‐throughput screening of very diverse glycoalkaloids. Glycosides of the same aglycone type were readily identified in the same transition window without the need for mass spectra interpretation. Validated using solamargine, the sole available standard, the accuracy was 99.7–101.3%, the intra‐ and inter‐day precision were, respectively, 2.5–5.0% and 8.0–9.2%, and the lower limit of detection and quantification were, respectively, 3.1 and 10.2 ng/mL (with 1 μL injection volume). Conclusions The peudo‐MS3 method allowed for high‐throughput targeted analysis with compact MRM transitions to address a large number of glycoalkaloids with diverse structures. This method could serve to meet the most heavy‐duty demand for rapid inspection of glycoalkaloids in SNBs. This method can be adopted and used by those involved in food safety and forensics, in developing food and industrial products and in genetics and breeding.

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“…For example, Navarro-González et al collected liver samples to study the hypocholesterolemic effect of a tomato juice, based on previous studies suggesting this effect due to the inhibitory action of HMG-CoA reductase (HMGCR) [30]. Yuan et al developed and applied a method to determine the bioavailability of raspberry ketone and its derived metabolites in plasma and brain of mice [31,32]. Despite the possibility of collecting different tissues and organs in animal models, they can normally only be collected once the animals are euthanized.…”

Section: Experimental Designsmentioning

confidence: 99%

Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

et al. 2022

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The study of the bioavailability of bioactive compounds is a fundamental step for the development of applications based on them, such as nutraceuticals, functional foods or cosmeceuticals. It is well-known that these compounds can undergo metabolic reactions before reaching therapeutic targets, which may also affect their bioactivity and possible applications. All recent studies that have focused on bioavailability and metabolism of phenolic and terpenoid compounds have been developed because of the advances in analytical chemistry and metabolomics approaches. The purpose of this review is to show the role of analytical chemistry and metabolomics in this field of knowledge. In this context, the different steps of the analytical chemistry workflow (design study, sample treatment, analytical techniques and data processing) applied in bioavailability and metabolism in vivo studies are detailed, as well as the most relevant results obtained from them.

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UHPLC-QqQ-MS/MS method development and validation with statistical analysis: Determination of raspberry ketone metabolites in mice plasma and brain

Cited by 12 publications

References 23 publications

Pharmacological Exploration of Phenolic Compound: Raspberry Ketone—Update 2020

Pharmacological Exploration of Phenolic Compound: Raspberry Ketone—Update 2020

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

Recent Analytical Approaches for the Study of Bioavailability and Metabolism of Bioactive Phenolic Compounds

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