UHPLC–MS and GC–MS phytochemical profiling, amelioration of pain and inflammation with chloroform extract of Funaria hygrometrica Hedw. via modulation of inflammatory biomarkers

Asif, Ayesha; Ishtiaq, Saiqa; Kamran, Sairah Hafeez; Waseem, Raazia; Fatima, Syeda Farheen; Rehman, Saira

doi:10.1007/s10787-023-01207-y

Cited by 3 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The total reaction volume of 15 μL, which included 10μL of SYBR Green mix, primers at a concentration of 0.5 mM each, and 1 μL of cDNA as a template with the appropriate primers and housekeeping genes (GAPDH) were employed as controls for comparing the relevant Cts of samples to controls. The amplification conditions were 95°C for 30 s and 40 cycles at 95°C for 5 s and 60°C for 20 s [ 35 ]. The primer sequence of the gene NF-κB, IL-8 and IL-6 are presented in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Modulatory effects of rutin and vitamin A on hyperglycemia induced glycation, oxidative stress and inflammation in high-fat-fructose diet animal model

Iqbal,

Hafeez Kamran,

Siddique

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

In the current study we investigated the impact of combination of rutin and vitamin A on glycated products, the glyoxalase system, oxidative markers, and inflammation in animals fed a high-fat high-fructose (HFFD) diet. Thirty rats were randomly divided into six groups (n = 5). The treatments, metformin (120 mg/kg), rutin (100 mg/kg), vitamin A (43 IU/kg), and a combination of rutin (100 mg/kg) and vitamin A (43 IU/kg) were given to relevant groups of rats along with high-fructose high-fat diet for 42 days. HbA1c, D-lactate, Glyoxylase-1, Hexokinase 2, malondialdehyde (MDA), glutathione peroxidase (GPx), catalase (CAT), nuclear transcription factor-B (NF-κB), interleukin-6 (IL-6), interleukin-8 (IL-8) and histological examinations were performed after 42 days. The docking simulations were conducted using Auto Dock package. The combined effects of rutin and vitamin A in treated rats significantly (p < 0.001) reduced HbA1c, hexokinase 2, and D-lactate levels while preventing cellular damage. The combination dramatically (p < 0.001) decreased MDA, CAT, and GPx in treated rats and decreased the expression of inflammatory cytokines such as IL-6 andIL-8, as well as the transcription factor NF-κB. The molecular docking investigations revealed that rutin had a strong affinity for several important biomolecules, including as NF-κB, Catalase, MDA, IL-6, hexokinase 2, and GPx. The results propose beneficial impact of rutin and vitamin A as a convincing treatment strategy to treat AGE-related disorders, such as diabetes, autism, alzheimer’s, atherosclerosis.

show abstract

Section: Methodsmentioning

confidence: 99%