UHPLC as a suitable methodology for the analysis of carotenoids in food matrix

Development and validation of a rapid resolution liquid chromatography method for the screening of dietary plant isoprenoids: Carotenoids, tocopherols and chlorophylls

“…For instance, lower RSD values have been reported for these compounds in other studies, although such results were obtained by analyzing a mixture of standards rather than by considering both the extraction and HPLC procedures [28]. In such case only the precision of the chromatographic method was taken into account and not that of the extraction process, which is more likely to produce analyte losses.…”

“…A recent study describes the analysis of a mixture of up to 16 CARS standards by UPLC-MS/MS in 12 min [27]. Similarly, Bohoyo-Gil et al [28] proposed a methodology by UPLC for the determination of the seven carotenoids in honeybee pollen samples, pumpkin and nectarine flesh with satisfactory recoveries in 10 min.…”

Effects of Light Intensity and Nitrogen Starvation on Glycerolipid, Glycerophospholipid, and Carotenoid Composition in Dunaliella tertiolecta Culture

“…A method based on Bohoyo-Gil et al [18] and Scaife et al [19] were modified to extract carotenoids from D. tertiolecta . Initially, 10 mg of dried D. tertiolecta sample was extracted with 1 mL of acetone and vortexed for 20 sec.…”

“…The column was selected as a Zorbax Eclipse plus C18 column, 2.1 mm×100 mm and 1.8 µm particle size from Agilent Technologies (Böblingen, Germany) [18], and the column oven was set to a temperature of 25°C. The temperature of the auto-sampler tray was set to 4°C.…”

“…One hundred micrograms per mililiter of standard stock solutions were prepared in hexane containing 0.1% BHT (w/v) in order to increase solution stability [18], [21]. Solutions were stored in amber vials at −80°C.…”