UhpA in Edwardsiella piscicida decreases the pathogenicity and the capability of inducing cytokine response in zebrafish

Liu, Jinyu; Sun, Yongcan; Huang, Jinjing; Ding, Lei; Ye, Tingqi; Wang, Xuepeng

doi:10.1016/j.aqrep.2020.100293

Cited by 7 publications

(12 citation statements)

References 28 publications

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“…One of them, the UhpA was regulated by the transport protein UhpT, to transport a broad range of phosphorylated sugars (15). In this system, the Glu6P has been used as a broad specificity of sugar phosphates (21), which decreased the pathogenicity of E. piscicida in zebrafish (15). Although UhpA decreased its pathogenicity has been reported, UhpA how to regulate the main virulent gene are still not available.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial strains include the wild type strain E. piscicida EIB202, the uhpA gene mutant strain ΔuhpA (15,25). E. piscicida strains were grown in tryptic soy broth (TSB) at 28℃.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

“…The pathogenesis of E. piscicida include many virulence factors such as the production of hemolysis (9,10), filament structural protein of flagellar (11), possession of type Ш secretion system (T3SS) (2,9), and type VI secretion system (T6SS) (12), production of growth metabolism (13,14), ability to adhere, invade, survive and replicate in host cells (2). Additionally, most of them were regulated by the two-component signal transduction system (TCS), such as UhpA/UhpB-UhpC, which enables the cell to acquire phosphorylated sugars from its environment that can be used as carbon or energy sources (15). Although uhpA gene deletion affects Glucose 6-phosphate (Glu6P) usage in E. piscicida and UhpA could decrease its pathogenicity in zebrafish (15), UhpA how to regulate the main virulent genes of hemolysis, flagellar, T3SS, T6SS and metabolism are still not available.…”

mentioning

confidence: 99%

“…Additionally, most of them were regulated by the two-component signal transduction system (TCS), such as UhpA/UhpB-UhpC, which enables the cell to acquire phosphorylated sugars from its environment that can be used as carbon or energy sources (15). Although uhpA gene deletion affects Glucose 6-phosphate (Glu6P) usage in E. piscicida and UhpA could decrease its pathogenicity in zebrafish (15), UhpA how to regulate the main virulent genes of hemolysis, flagellar, T3SS, T6SS and metabolism are still not available.…”

mentioning

confidence: 99%

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Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Chen¹,

Sun²,

Ye³

et al. 2020

Preprint

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Edwardsiella piscicida (E. piscicida) is an important zoonotic pathogen, which infects animals by colonizing the intestine. Glucose 6-phosphate (Glu6P) was an important carbohydrate in intestine and could be used as a regulate signal. Here we identify a virulence-regulating pathway named Glu6P transport regulatory protein UhpA, which affects the virulent genes of hemolysins, flagellar, T3SS, T6SS and metabolism related genes how to promote E. piscicida infect the host. The results showed that the metabolism related gene expression of cysteine synthase (orf 1134) and sulfate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76-fold and 0.68-fold lower than the ones in the wild strains (P < 0.05), the gene expression of ethA and ethB in the ΔuhpA strain was 0.80-fold and 0.72-fold lower than the ones in the wild strains (P < 0.05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51-fold and 1.21-fold higher than the ones in the wild strains (P < 0.05), the gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27-fold, 1.13-fold 1.28-fold and 1.23-fold higher than the ones in the wild strains (P < 0.05). Besides, the survival rate of fish challenged with E. piscicida EIB202 and ΔuhpA was 50% and 30% respectively. These suggested that although the uhpA gene deletion decreased the metabolic level and the hemolysins related gene expression in E. piscicida, the uhpA gene could down regulate the key virulent gene expression to decrease the pathogenicity of E. piscicida in fish.

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Section: Discussionmentioning

confidence: 99%

“…Bacterial strains include the wild type strain E. piscicida EIB202, the uhpA gene mutant strain ΔuhpA (15,25). E. piscicida strains were grown in tryptic soy broth (TSB) at 28℃.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Chen¹,

Sun²,

Ye³

et al. 2020

Preprint

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“…Total RNA extraction from head kidney and spleen was performed as described previously research (Sun, Li, et al., 2020), and qRT‐PCR was carried out as described previously with β‐actin as an internal reference (Liu et al., 2020; Wang, Wang, et al., 2020). Primers for immune‐related genes are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

Wang

Zhang

et al. 2020

Aquaculture Research

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Streptococcus iniae (S. iniae) is a Gram‐positive bacterium associated with disease in several commercial species and other animals including humans. This zoonotic pathogen has led to high worldwide morbidity and mortality and huge economic losses in aquaculture. Here a S. iniae ghost (SIG) vaccine was successfully generated with a chemical method, and its immunogenicity and protective efficacy were examined in this study. The results showed that the titres of immunoglobulin M (IgM) in serum were significantly higher in group A (SIG‐immunized) than group B (formalin‐inactivated S. iniae, FSI‐immunized), which was significantly higher than the control group (PBS‐treated). Also, the phagocytic activity was significantly higher in group A than group B, which was significantly higher than the control group. Moreover, AKP activity and lysozyme activity of serum in group A were significantly higher than group B, which was significantly higher than the control group. Also, IL‐1β, IFN‐γ and HSP70 in the head kidney of fish immunized with SIG and FSI showed significantly higher than the control group throughout the whole experimental period. In addition, IL‐1β and IFN‐γ in the spleen of fish immunized with SIG showed significantly higher than that of in the FSI‐immunized fish. Most important, the survival rate and relative per cent survival of group A were higher than group B, and both of them were all significantly more protected against the S. iniae challenge compared to the PBS‐treated group. These results suggest that immunization with SIG induces immune responses and provides protection against a virulent S. iniae challenge.

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FucP mediates fucose to regulate T3SS in Edwardsiella piscicida and promotes intestinal colonization in Tilapia

et al. 2021

Aquacult Int

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UhpA in Edwardsiella piscicida decreases the pathogenicity and the capability of inducing cytokine response in zebrafish

Cited by 7 publications

References 28 publications

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Glucose 6-phosphate transport regulatory protein UhpA regulates the virulent genes in Edwardsiella piscicida

Characterization ofStreptococcus iniaeghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus)

FucP mediates fucose to regulate T3SS in Edwardsiella piscicida and promotes intestinal colonization in Tilapia

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