Uga3 and Uga35/Dal81 Transcription Factors Regulate
            <i>UGA4</i>
            Transcription in Response to γ-Aminobutyric Acid and Leucine

Cardillo, Sabrina Beatriz; Moretti, Mariana Bermúdez; Garcia, Susana Raquel Correa

doi:10.1128/ec.00117-10

Cited by 18 publications

(45 citation statements)

References 49 publications

(58 reference statements)

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“…We also assayed the recruitment of Dal81 at the UGA1 promoter by ChIP. No enrichment of HA-Dal81 at UGA1 was detected in the absence of GABA while addition of the inducer allowed recruitment of this factor ( Figure 5, column 3), in agreement with another study performed with the UGA4 promoter (Cardillo et al 2010). As shown above, Gal11, a component of the mediator complex, is required for full activation by Uga3.…”

Section: Cofactors Involved In Uga3 Transcriptional Activationsupporting

confidence: 91%

Yeast Zinc Cluster Proteins Dal81 and Uga3 Cooperate by Targeting Common Coactivators for Transcriptional Activation of Γ-Aminobutyrate Responsive Genes

Sylvain¹,

Liang²,

Hellauer³

et al. 2011

Genetics

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In Saccharomyces cerevisiae, optimal utilization of various compounds as a nitrogen source is mediated by a complex transcriptional network. The zinc cluster protein Dal81 is a general activator of nitrogen metabolic genes, including those for g-aminobutyrate (GABA). In contrast, Uga3 (another zinc cluster protein) is an activator restricted to the control of genes involved in utilization of GABA. Uga3 binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA. Dal81 appears to act as a coactivator since the DNA-binding activity of this factor is dispensable but its mode of action is not known. In this study, we have mapped a regulatory, as well as an activating, region for Uga3. A LexA-Uga3 chimeric protein activates a lexA reporter in a GABA-and Dal81-dependent manner. Activation by Uga3 requires the SAGA complex as well as Gal11, a component of mediator. ChIP analysis revealed that Uga3 is weakly bound to target promoters. The presence of GABA enhances binding of Uga3 and allows recruitment of Dal81 and Gal11 to target genes. Recruitment of Gal11 is prevented in the absence of Dal81. Importantly, Dal81 by itself is a potent activator when tethered to DNA and its activity depends on SAGA and Gal11 but not Uga3. Overexpression of Uga3 bypasses the requirement for Dal81 but not for SAGA or Gal11. Thus, under artificial conditions, both Dal81 and Uga3 can activate transcription independently of each other. However, under physiological conditions, both factors cooperate by targeting common coactivators.

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Section: Cofactors Involved In Uga3 Transcriptional Activationsupporting

confidence: 91%

Yeast Zinc Cluster Proteins Dal81 and Uga3 Cooperate by Targeting Common Coactivators for Transcriptional Activation of Γ-Aminobutyrate Responsive Genes

Sylvain¹,

Liang²,

Hellauer³

et al. 2011

Genetics

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“…First, a strain containing an insertion of the KanMX-KlURA3 module was generated using the pCORE plasmid as template for PCR (Storici et al, 2001;Storici & Resnick, 2003). Second, fragments containing the desired mutations in the promoter of UGA4 gene were generated by PCR using the plasmids Yep357-UAS GABA mut and Yep357-UAS GABA del (Cardillo et al, 2010) as templates. Counter-selection with 5-fluorootic acid (5-FOA) was carried out to isolate those mutants that had lost the pCORE module and had incorporated the desired mutations.…”

Section: Methodsmentioning

confidence: 99%

“…ChIP experiments were performed according to the method of Cardillo et al (2010). Normal mouse IgG (Santa Cruz) or monoclonal anti-HA antibody [HA probe (F-7), Santa Cruz] were used.…”

Section: Methodsmentioning

confidence: 99%

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Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters

et al. 2012

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c-Aminobutyric acid (GABA) transport and catabolism in Saccharomyces cerevisiae are subject to a complex transcriptional control that depends on the nutritional status of the cells. The expression of the genes that form the UGA regulon is inducible by GABA and sensitive to nitrogen catabolite repression (NCR). GABA induction of these genes is mediated by Uga3 and Dal81 transcription factors, whereas GATA factors are responsible for NCR. Here, we show how members of the UGA regulon share the activation mechanism. Our results show that both Uga3 and Dal81 interact with UGA genes in a GABA-dependent manner, and that they depend on each other for the interaction with their target promoters and the transcriptional activation. The typical DNA-binding domain Zn(II) 2 -Cys 6 of Dal81 is unnecessary for its activity and Uga3 acts as a bridge between Dal81 and DNA. Both the trans-activation activity of the GATA factor Gln3 and the repressive activity of the GATA factor Dal80 are exerted by their interaction with UGA promoters in response to GABA, indicating that Uga3, Dal81, Gln3 and Dal80 all act in concert to induce the expression of UGA genes. So, an interplay between the factors responsible for GABA induction and those responsible for NCR in the regulation of the UGA genes is proposed here.

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“…c GAAC-regulated expression according to Natarajan et al (2001). (Cai et al 2007), and Uga3-dependent gene expression of UGA4, the GABA permease (Cardillo et al 2010).…”

Section: Pathway Genesmentioning

confidence: 99%

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

2012

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Ever since the beginning of biochemical analysis, yeast has been a pioneering model for studying the regulation of eukaryotic metabolism. During the last three decades, the combination of powerful yeast genetics and genome-wide approaches has led to a more integrated view of metabolic regulation. Multiple layers of regulation, from suprapathway control to individual gene responses, have been discovered. Constitutive and dedicated systems that are critical in sensing of the intra- and extracellular environment have been identified, and there is a growing awareness of their involvement in the highly regulated intracellular compartmentalization of proteins and metabolites. This review focuses on recent developments in the field of amino acid, nucleotide, and phosphate metabolism and provides illustrative examples of how yeast cells combine a variety of mechanisms to achieve coordinated regulation of multiple metabolic pathways. Importantly, common schemes have emerged, which reveal mechanisms conserved among various pathways, such as those involved in metabolite sensing and transcriptional regulation by noncoding RNAs or by metabolic intermediates. Thanks to the remarkable sophistication offered by the yeast experimental system, a picture of the intimate connections between the metabolomic and the transcriptome is becoming clear.

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Uga3 and Uga35/Dal81 Transcription Factors Regulate UGA4 Transcription in Response to γ-Aminobutyric Acid and Leucine

Cited by 18 publications

References 49 publications

Yeast Zinc Cluster Proteins Dal81 and Uga3 Cooperate by Targeting Common Coactivators for Transcriptional Activation of Γ-Aminobutyrate Responsive Genes

Yeast Zinc Cluster Proteins Dal81 and Uga3 Cooperate by Targeting Common Coactivators for Transcriptional Activation of Γ-Aminobutyrate Responsive Genes

Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

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