UFLC Method Development for the Quantification of Carbamazepine in Blood Samples and an Application to Pharmacokinetic Study

Abstract

An isocratic simple rapid method has been developed and validated for the determination of carbamazepine (CBZ) in both solution form and blood samples. The assay was performed using an analytical C18 (100 mm×4.6 mm i. d) with a mobile phase consisting of methanol and water (50:50), the flow rate was 1 ml/min and UV detection at 285 nm. The method was found to be specific for CBZ, no interfering peaks were observed with an overall analytical run time of 5 min. Accuracy reported as % recovery were found to be 95.83-103.34% and 93.18-104.49% for inter-day and intra-day accuracies, respectively. Inter-day precision (reproducibility) was found to be 0.22-1.19 SD, while intra-day precision (repeatability) was found to be 0.14-2.12 SD for the samples studied. The calibration curve was found to be linear with the equation y=13474x+16969, with a correlation coefficient of 0.997 (R2) over a concentration range of 0.5-40 lg/ml. The farthest point of quantitation was the least fixation. The system is straightforward and fast and does not require any preparatory treatment of the specimen. The strategy was approved and effectively requested the blood tests to study the medication pharmacokinetic parameters.