UDP-glycosyltransferases play a crucial role in the accumulation of alkaloids and sesquiterpene glycosides in Dendrobium nobile

Tan, Daopeng; Wang, Jianmei; Cao, Ligang; Da, Yang; Lu, Yanliu; Wu, Di; Zhao, Yongxia; Wu, Xingdong; Fan, Qingjie; Yang, Zhou; Qin, Lin; He, Yuqi

doi:10.1016/j.arabjc.2023.104673

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…In addition, some studies have shown that the increase in SG contents from D. nobile may be caused by the enrichment of UDP glycosyltransferases involved in SG synthesis. 32 Considering that the production area may affect the contents of components of Dendrobium officinale under different epiphytic substrate cultivation modes were also different. 35 Accordingly, combining with the medicinal components of D. nobile, as well as the needs of planting costs and conditions, Guizhou may be more suitable for planting D. nobile than Hainan.…”

Section: Principal Component Analysismentioning

confidence: 99%

Quantitative analysis of six sesquiterpene glycosides from Dendrobium nobile Lindl. under different growth conditions by high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry in MRM mode

Gao,

Wu,

Yang

et al. 2024

Phytochemical Analysis

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The contents of sesquiterpene glycosides (SGs) from Dendrobium nobile Lindl. were greatly influenced by different growth conditions. A quantitative method based on HPLC‐QqQ‐MS/MS in MRM mode was developed for simultaneous analysis of six SGs. The results were further analyzed by principal component analysis and hierarchical cluster analysis. The results showed the SG contents were relatively high in 3‐year‐old D. nobile grown at an altitude of approximately 500 m on Danxia rocks in Chishui, Guizhou.

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Section: Principal Component Analysismentioning

confidence: 99%

Quantitative analysis of six sesquiterpene glycosides from Dendrobium nobile Lindl. under different growth conditions by high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry in MRM mode

Gao,

Wu,

Yang

et al. 2024

Phytochemical Analysis

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“…The control of polysaccharide production in plants is achieved by the regulation of GTs. This regulation encompasses various processes, including gene expression, protein modification, and enzyme activity regulation ( Tan et al, 2023 ). The availability and activity of nucleotide sugar donors, which are generated in the cytosol and transferred to the Golgi apparatus, significantly affect GTs activity.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, GTs activity can be influenced by post-translational changes such as phosphorylation, acetylation, and glycosylation, which can either enhance or limit the enzyme’s activity. Environmental factors such as temperature, light, and nutrition availability also influence the control of GTs activity and the production of polysaccharides ( Tan et al, 2023 ). Nucleotide sugar transporters facilitate the transportation of UDP monosaccharides and GDP monosaccharides from the cytoplasm of plants to the Golgi apparatus.…”

Section: Introductionmentioning

confidence: 99%

“…There are two basic forms of GTs that play a role in polysaccharide biosynthesis: one has a single transmembrane domain, while the other has numerous transmembrane domains ( Zabotina, Zhang & Weerts, 2021 ). Annotating GT family genes associated with plant polysaccharide synthesis has been performed in the CAZY database ( http://www.cazy.org/ ) ( Tan et al, 2023 ). Within this group, the GT1 family has the highest number of members and the most varied range of functions.…”

Section: Introductionmentioning

confidence: 99%

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Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Xu,

Hu,

et al. 2024

PeerJ

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Tuber plants are of great significance in the world as human food crops. Polysaccharides, important metabolites in tuber plants, also serve as a source of innovative drugs with significant pharmacological effects. These drugs are particularly known for their immunomodulation and antitumor properties. To fully exploit the potential value of tuber plant polysaccharides and establish a synthetic system for their targeted synthesis, it is crucial to dissect their metabolic processes and genetic regulatory mechanisms. In this article, we provide a comprehensive summary of the basic pathways involved in the synthesis of various types of tuber plant polysaccharides. We also outline the key research progress that has been made in this area in recent years. We classify the main types and functions of tuber plant polysaccharides and analyze the biosynthetic processes and genetic regulation mechanisms of key enzymes involved in the metabolic pathways of starch, cellulose, pectin, and fructan in tuber plants. We have identified hexokinase and glycosyltransferase as the key enzymes involved in the polysaccharide synthesis process. By elucidating the synthesis pathway of polysaccharides in tuber plants and understanding the underlying mechanism of action of key enzymes in the metabolic pathway, we can provide a theoretical framework for enhancing the yield of polysaccharides and other metabolites in plant culture cells. This will ultimately lead to increased production efficiency.

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“…Plant UGTs are a class of enzymes found in plant cells that have the ability to transfer various glycosyl groups to substrate molecules, hence altering their biological activity and characteristics . Many bioactive compounds, including alkaloids, saponins, and steroids, are produced by the catalysis of plant UGTs. − The glycosylation process generally occurs at functional groups such as hydroxyl, amino, carboxyl, thiol groups or even carbon atoms of the substrate molecules, and the resulting glycosylated relatives could be endowed with reduced toxicity and enhanced biological activity . In addition, the use of plant UGTs also allows the synthesis of non-natural products, which are new natural active substances with different activities and potencies from the original compounds .…”

Section: Introductionmentioning

confidence: 99%

Substrate Promiscuity, Crystal Structure, and Application of a Plant UDP-Glycosyltransferase UGT74AN3

Huang,

Zhang,

et al. 2023

ACS Catal.

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Glycosyltransferases are effective enzymes for glycosylating natural products (NPs), and some of them have the unusual property of being exceedingly promiscuous catalytically toward a range of substrates. UGT74AN3 is a plant glycosyltransferase identified from Catharanthus roseus in our previous work. In this study, we found that UGT74AN3 exhibits high substrate promiscuity toward 78 acceptors and 6 sugar donors and also exhibits N-/S-glycosylation activity toward simple aromatic compounds. The crystal structures of UGT74AN3 in the complex with various NPs were solved. Sugar donor recognition of UGT74AN3 was altered by structure-based mutagenesis, and the T145V mutant shifted its sugar donor preference from UDP-Glc to UDP-Xyl. Structural analysis reveals that a spacious U-shaped hydrophobic binding pocket accounts for the high substrate promiscuity of UGT74AN3. The residues E85 and F193 might serve as gatekeepers of UGT74AN3 to control substrate binding. In addition, a rare substrate binding mode was discovered in the structure of UGT74AN3, and the process of substrate flipping in the pocket was charted by molecular dynamics simulations. Moreover, a cost-effective one-pot system by coupling UGT74AN3 with AtSuSy, a sucrose synthase, was established for in situ generating and recycling UDP-Glc from sucrose and UDP to glycosylate NPs. Our study reveals the structural basis underlying the substrate promiscuity of UGT74AN3 and provides an efficient and economical enzymatic synthesis strategy for producing valuable glycosides for drug discovery.

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UDP-glycosyltransferases play a crucial role in the accumulation of alkaloids and sesquiterpene glycosides in Dendrobium nobile

Cited by 8 publications

References 16 publications

Quantitative analysis of six sesquiterpene glycosides from Dendrobium nobile Lindl. under different growth conditions by high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry in MRM mode

Quantitative analysis of six sesquiterpene glycosides from Dendrobium nobile Lindl. under different growth conditions by high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry in MRM mode

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Substrate Promiscuity, Crystal Structure, and Application of a Plant UDP-Glycosyltransferase UGT74AN3

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