UDP-glucuronyltransferase in perfused rat liver and in microsomes: V. Studies with gunn rats

Bock, Karl Walter; Clausbruch, Ulrich C.V.; Ottenwälder, H.

doi:10.1016/0006-2952(78)90245-9

Cited by 19 publications

(1 citation statement)

References 22 publications

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“…Phosphatidylcholine or microsomal protein (mg/mg of protein in enzyme preparation) Fig. 1 (Zakim et al, 1973); this has not been confirmed by more recent studies (Bock et al, 1978;Leakey, 1978;Burchell & Bock, 1980;Lalani et al, 1980). Furthermore the demonstration here that Gunnor Wistar-rat liver microsomes are equally effective environments for restoration of activity to highly purified bilirubin UDP-glucuronyltransferase suggests that the Gunn-rat hepatic microsome is not an abnormal environment.…”

Section: Discussionmentioning

confidence: 61%

Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferase into Gunn-rat liver microsomes

Burchell¹

1982

Biochemical Journal

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1. Reconstitution of purified bilirubin UDP-glucuronyltransferase from Wistar-rat liver into Gunn-rat liver microsomes provides a better environment than phosphatidylcholine liposomes, such that the final specific activity of the Wistar-rat liver enzyme was increased up to 85 units/mg of protein. 2. Gunn- and Wistar-rat liver microsomes were equally effective for reconstitution of the purified enzyme. 3. The transferase activity does not appear to be fully expressed in the more rigid environment of foetal Wistar-rat liver microsomes. 4. These reconstitution experiments reveal a final specific activity for the purified bilirubin UDP-glucuronyltransferase consistent with the capacity of the whole rat liver to glucuronidate bilirubin and indicate that the absence of this enzyme activity in Gunn-rat liver microsomes is not due to an abnormal microenvironment.

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Section: Discussionmentioning

confidence: 61%

Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferase into Gunn-rat liver microsomes

Burchell¹

1982

Biochemical Journal

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Hepatic Disposition and Elimination of Biliary Contrast Media

Barnhart¹

1984

Radiocontrast Agents

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Vitamin A (retinol) status in the Gunn rat

Thunberg

Håkansson

1983

Arch Toxicol

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Vitamin A (retinol) status and the effect of a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 10 micrograms X kg-1, on vitamin A in the liver and serum, and on the hepatic UDP-glucuronosyltransferase (UDPGT) (EC 2.4.1.17) activity, were studied in heterozygous (GW) and homozygous (GG) Gunn rats. 1) Data from vitamin A analyses demonstrate that the amount of vitamin A stored in the liver of untreated Gunn rats is of the same magnitude as that of Sprague-Dawley rats. 2) The retinol content in the liver of both GG and GW rats was reduced to about 50% by TCDD-treatment. 3) Retinol levels in serum were found to be variable and no significant effect due to TCDD could be observed. 4) No correlation between the TCDD-induced reduction of vitamin A and the induction of UDPGT activity by TCDD could be demonstrated in this study. The vitamin A reduction caused by TCDD was considerably less in the Gunn rat than in the Sprague-Dawley rat, and the results indicate that the Gunn rat is more resistant to TCDD than other strains of rat. TCDD-induced reduction of liver vitamin A seems to some extent to correlate with TCDD-toxicity in different strains of rat. The specific properties of the Gunn rat and its relatively high resistance to TCDD make it a valuable tool in studies about the mechanism of TCDD-toxicity.

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UDP-glucuronyltransferase in perfused rat liver and in microsomes: V. Studies with gunn rats

Cited by 19 publications

References 22 publications

Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferase into Gunn-rat liver microsomes

Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferase into Gunn-rat liver microsomes

Hepatic Disposition and Elimination of Biliary Contrast Media

Vitamin A (retinol) status in the Gunn rat

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