UDP-glucosyltransferase OsUGT75A promotes submergence tolerance during rice seed germination

Abstract

Submergence stress represents a major obstacle limiting the application of direct seeding in rice cultivation. Under flooding conditions, coleoptile elongation can function as an escape strategy that contributes to submergence tolerance during seed germination in rice; however, the underlying molecular bases have yet to be fully determined. Herein, we report that natural variation of rice coleoptile length subjected to submergence is determined by the glucosyltransferase encoding gene OsUGT75A. OsUGT75A regulates coleoptile length via decreasing free abscisic acid (ABA) and jasmonic acid (JA) levels by promoting glycosylation of these two phytohormones under submergence. Moreover, we find that OsUGT75A accelerates coleoptile length through mediating the interactions between JASMONATE ZIMDOMAIN (OsJAZ) and ABSCISIC ACID-INSENSITIVE (OsABI) proteins. Last, we reveal the origin of the haplotype that contributes to coleoptile length in response to submergence and transferring this haplotype to indica rice can enhance coleoptile length in submergence conditions. Thus, we propose that OsUGT75A is a useful target in breeding of rice varieties suitable for direct seeding cultivation.

Dynamic monitoring of TGW6 by selective autophagy during grain development in rice

“…The recombinant proteins (GST‐OsATG8s, TGW6 ▵AIM2 ‐MBP, TGW6 ▵AIM3 ‐MBP, TGW6‐MBP, GST, and MBP) were purified from Escherichia coli strain BL21 and used for GST pull‐down assays as reported previously (He et al ., 2023). Detection of GST‐ and MBP‐fused proteins was performed with anti‐GST (CW0084M; 1 : 5000; CWBIO, Beijing, China) and anti‐MBP antibodies (E8032S; 1 : 5000; New England Biolabs, Ipswich, MA, USA), respectively.…”

Comprehensive gene expression analysis of submergence stress response in nucleus-cytoplasm hybrid wheat

“…Jasmonic acid has been shown in Arabidopsis to be an essential phytohormone that activates the ROS detoxification system (Yuan et al 2017). Recently, it was shown that UDP-glucosyltransferase promotes submergence tolerance in rice during seed germination via decreasing free jasmonic acid and abscisic acid levels by promoting glycosylation of these phytohormones under submergence (He et al 2023). The significant enrichment of "GO:0009753; response to jasmonic acid" in DEGs detected in the present study might possibly suggest that transcription of genes suppressing jasmonic acid synthesis is down-regulated under submergence stress in wheat.…”

Morphological and molecular response mechanisms of the root system of different Hemarthria compressa species to submergence stress

“…A study identified a key gene, OsUGT75A (encoding a UDP-glucosyltransferase), that controls the rapid elongation of rice germ sheaths under flooded conditions. This gene precisely regulates the free ABA and JA contents in seeds and germ sheaths through glycosylation of abscisic acid (ABA) and jasmonic acid (JA), thereby mediating the elongation of germ sheaths through the ABA and JA signaling pathways, which further reveals the molecular mechanism of rice flooding tolerance through the “escape strategy” ( He et al., 2023 ). Transcriptomic analysis of root tissues of flood-tolerant and flood-sensitive orchardgrass ( Dactylis glomerata ) cultivars, following submergence stress, identified DEGs of phytohormone-related genes, mitogen-activated protein kinases (MAPK), and Ca 2+ signaling genes that were significantly differentially expressed in submergence-tolerant orchardgrass ( Qu et al., 2023 ).…”