UCP3: An Uncoupling Protein Homologue Expressed Preferentially and Abundantly in Skeletal Muscle and Brown Adipose Tissue

Vidal-Puig, António; Solanes, Gemma; Grujić, Danica; Flier, Jeffrey S.; Lowell, Bradford B.

doi:10.1006/bbrc.1997.6740

Cited by 672 publications

(457 citation statements)

References 17 publications

Supporting

Mentioning

427

Contrasting

Unclassified

Order By: Relevance

“…It has been demonstrated that UCP2 are expressed in a wide variety of tissues, while UCP3 specifically in muscle and BAT [3,5,7,22]. Although essentially the same tissue distribution was confirmed in the present study both in obese yellow KK and control C57BL mice, a noticeable difference in the expression level of UCP2 mRNA was found between these two mouse strains: that is, the UCP2 mRNA level in adipose tissues and skeletal muscle was higher in the obese mice.…”

Section: Discussionsupporting

confidence: 72%

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

Nakamura

Nagase

Asano

et al. 2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Chronic stimulation of the β3-adrenergic receptor (AR) in obese animals resulted in a reduced adiposity associated with an increased expression of thermogenic uncoupling protein (UCP)1 in adipose tissues. In this study, the mRNA expression of newly cloned UCP isoforms (UCP2 and UCP3) were examined in obese yellow KK and C57BL control mice. UCP2 mRNA was found in all tissues examined, with higher levels in adipose tissues and skeletal muscle of the obese mice. UCP3 mRNA was expressed in skeletal muscle, heart and brown adipose tissue similarly in the two mouse strains. Daily injection of a selective β3-adrenergic agonist, CL316,243 (0.1 mg/kg), for 10 days resulted in a marked reduction of white fat pad weight and 1.8~4.8-fold increase in the mRNA levels of UCP2 and UCP3 in skeletal muscle of obese mice. No noticeable change in the UCP2 and 3 mRNA levels was found in brown and white adipose tissues. It was also found that CL316,243 injection produced a marked and sustained elevation of the plasma free fatty acid level. These results, together with our previous findings of the fatty acid-induced UCP expression in a myocyte cell line in vitro, suggest that the β3-AR agonist-induced UCP expression in skeletal muscle may be mediated through the elevated plasma free fatty acids. It was also suggested that anti-obesity effect of β3-AR agonists is attributable to increased thermogenesis not only by UCP1 but also by UCP2 and UCP3.KEY WORDS: adipose tissue, β3-adrenergic agonist, fatty acid, obese mouse, uncoupling protein.

show abstract

Section: Discussionsupporting

confidence: 72%

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

Nakamura

Nagase

Asano

et al. 2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…4 UCP3 has significant amino-acid homology to UCP2 (71%), and expression of UCP3 in humans is much greater in skeletal muscle than in other tissues. 5 UCP2 mRNA levels decrease in white adipose tissue in obese subjects compared with their lean control counterparts. 6 UCP2 downregulates insulin secretion to alter b-cell lipid metabolism 7 or to reduce ATP production.…”

Section: Introductionmentioning

confidence: 87%

UCP2 A55V variant is associated with obesity and related phenotypes in an aboriginal community in Taiwan

et al. 2007

View full text Add to dashboard Cite

Objective: Human uncoupling proteins 2 and 3 (UCP2 and UCP3) are two mitochondrial proteins that are involved in the control of metabolism of fatty acid and possibly protect against oxidative damage. The aim of this study was to analyze genetic associations of four polymorphisms of the UCP2 and UCP3 genes with insulin, leptin concentration and obesity in Taiwan aborigines.Research methods: Four polymorphisms were compared in 324 obese (body mass index (BMI) X30 kg/m 2 ) and overweight (304BMI X25 kg/m 2 ) subjects, and 114 normal weight subjects (BMI o25 kg/m 2 ) in an aboriginal community of southern Taiwan. Anthropometric characteristics and fasting levels of insulin, leptin, triglycerides and cholesterol were measured. Results: Before and after adjusting for age distribution, only the Val55 allele in exon 4 of the UCP2 gene increased the risk of overweight and obesity (adjusted odds ratio (OR) ¼ 2.02, P ¼ 0.004) in comparison with Ala55. UCP2 V55V is also associated with higher fasting insulin levels than A55V (P ¼ 0.01) and A55A (P ¼ 0.04) in the obese/overweight group. Using the COCAPHASE program of the UNPHASED software, haplotype analysis of three single nucleotide polymorphisms (A55V-G866A-C-55T) revealed that A-G-C (73% in obese subjects and 77% in controls) was the most common haplotype and that the haplotype V-A-T (13% in obese subjects and 5% in controls) was significantly increased in obese and overweight subjects (BMI X25 kg/m 2 ) (OR ¼ 2.62, Po0.001). Discussions: UCP2 A55V variant might predispose to obesity and Val55 allele to confer population-attributable risk for 9.5% of obese disorders and increase insulin concentrations. The V-A-T haplotype within UCP2-UCP3 gene cluster is also significantly associated with obesity in Paiwan aborigines.

show abstract

“…There is also an inhibition of proton conductance in yeast mitochondria by ATP [28]. UCP3 is a UCP1 homologue that is highly skeletal-muscle-specific [29,30]. Its uncoupling activity is a matter of debate [4][5][6]10,12].…”

Section: Effects Of Nucleotides On the Proton Conductance Of Mitochonmentioning

confidence: 99%

Effects of magnesium and nucleotides on the proton conductance of rat skeletal-muscle mitochondria

Cadenas

Brand

2000

Biochemical Journal

View full text Add to dashboard Cite

During oxidative phosphorylation most of the protons pumped out to the cytosol across the mitochondrial inner membrane return to the matrix through the ATP synthase, driving ATP synthesis. However, some of them leak back to the matrix through a proton-conductance pathway in the membrane. When the ATP synthase is inhibited with oligomycin and ATP is not being synthesized, all of the respiration is used to drive the proton leak. We report here that Mg# + inhibits the proton conductance in rat skeletal-muscle mitochondria. Addition of Mg# + inhibited both oligomycin-inhibited respiration and the proton conductance, while removal of Mg# + using EDTA activated these processes. The proton conductance was inhibited by more than 80 % as free Mg# + was raised from 25 nM to 220 µM. Half-maximal inhibition occurred at about 1 µM free Mg# + ,

show abstract

UCP3: An Uncoupling Protein Homologue Expressed Preferentially and Abundantly in Skeletal Muscle and Brown Adipose Tissue

Cited by 672 publications

References 17 publications

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

.BETA.3-Adrenergic Agonist Up-Regulates Uncoupling Proteins 2 and 3 in Skeletal Muscle of the Mouse.

UCP2 A55V variant is associated with obesity and related phenotypes in an aboriginal community in Taiwan

Effects of magnesium and nucleotides on the proton conductance of rat skeletal-muscle mitochondria

Contact Info

Product

Resources

About