UCP-LF and other assay methods for schistosome circulating anodic antigen between 1978 and 2022

Fasogbon, Ilemobayo Victor; Aja, P. M.; Ondari, Erick Nyakundi; Adebayo, Ismail Abiola; Ibitoye, Olukayode Adebola; Egesa, Moses; Tusubira, Deusdedit; Swamiappan, Sasikumar; Onohuean, Hope

doi:10.1093/biomethods/bpad006

Cited by 7 publications

(6 citation statements)

References 92 publications

(38 reference statements)

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“…In general, LFAs offer significant advantages in diagnostics, making them particularly suitable for POC applications. Such advantages include: the fact that they are often designed for ease of use in various settings, including remote or resource-limited areas [ 9 , 59 ]. They provide rapid results at the POC, facilitating quick decision-making without the need to send samples to a centralized laboratory.…”

Section: Resultsmentioning

confidence: 99%

Point-of-care potentials of lateral flow-based field screening for Mycoplasma bovis infections: a literature review

Fasogbon,

Ondari,

Deusdedit

et al. 2024

Biology Methods and Protocols

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Point-of-care (POC) field screening for tools for Mycoplasma bovis (M. bovis) is still lacking due to the requirement for a simple, robust field-applicable test that does not entail specialized laboratory equipment. In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines, this review identifies the methodologies that were retrieved based on our search strategy, that have been reported for the diagnosis of m. bovis infection between 2014 and diagnostics. A search criteria was generated to curate one hundred and three articles, which were reduced in number (to 46), following the screening guidelines of PRISMA. The 43 articles included in the study present twenty-five different assay methods. The assay methods were grouped as microbiological culture, serological assay, PCR-based assay, LAMP-based assay, NGS-based assay, or Lateral flow assay. We, however, focus our discussion on the three lateral flow-based assays relative to others, highlighting the advantages they present above the other techniques and their potential applicability as a POC diagnostic test for M. bovis infections. We therefore call for further research on developing a lateral flow-based screening tool that could revolutionize the diagnosis of M. bovis infection.

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Section: Resultsmentioning

confidence: 99%

Point-of-care potentials of lateral flow-based field screening for Mycoplasma bovis infections: a literature review

Fasogbon,

Ondari,

Deusdedit

et al. 2024

Biology Methods and Protocols

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“…Hence, the prevalence of microhaematuria and S. haematobium infections in our study should be interpreted in light of the limited accuracy of the applied diagnostics. While there are diagnostic approaches that more sensitively detect S. haematobium infections, for example, the circulating anodic antigen (CAA) based up-converting phosphor-lateral flow (UCP-LF) assay, they are laboratory tests that require expensive consumables and equipment and are not applicable at the point of care [ 41 – 43 ]. Taken together, there is a pressing need for the development of more sensitive and specific point-of-care diagnostic tools, which can be supplied together with praziquantel to health facilities in Pemba and other African regions if they are supposed to play a role in schistosomiasis control and elimination.…”

Section: Discussionmentioning

confidence: 99%

Capacities and needs of health care facilities for schistosomiasis diagnosis and management in elimination settings

Ndum,

Trippler,

Mohammed

et al. 2024

Parasites Vectors

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Background Schistosomiasis is a debilitating neglected tropical disease endemic in sub-Saharan Africa. The role of health facilities in the prevention, diagnosis, control, and elimination of schistosomiasis is poorly documented. In a setting targeted for schistosomiasis elimination in Zanzibar, we assessed the prevalence of Schistosoma haematobium among patients seeking care in a health facility and investigated schistosomiasis-related knowledge of staff, and health facilities’ capacities and needs for schistosomiasis diagnosis and management. Methods We conducted a health facility-based mixed-method study on Pemba Island from June to August 2023. Patients aged ≥ 4 years seeking care in four health facilities were screened for S. haematobium infection using urine filtration and reagent strips. Those patients aged ≥ 10 years were additionally interviewed about signs and symptoms. Staff from 23 health facilities responded to a questionnaire assessing knowledge and practices. Ten staff participated in a focus group discussion (FGD) about capacities and needs for schistosomiasis diagnosis and management. Results The prevalence of S. haematobium infection in patients attending the health facilities, as determined by the presence of eggs in urine, was 1.1% (8/712). Microhaematuria was detected in 13.3% (95/712) of the patients using reagent strips. Among patients responding to the questionnaire, pelvic pain, pain during sex, and painful urination were reported by 38.0% (237/623), 6.3% (39/623), and 3.2% (20/623), respectively. Among the health facility staff, 90.0% (44/49) and 87.8% (43/49) identified blood in urine and pelvic pain, respectively, as symptoms of urogenital schistosomiasis, 81.6% (40/49) and 93.9% (46/49) reported collecting a urine sample and pursuing a reagent strip test, respectively, for diagnosis, and 87.8% (43/49) administered praziquantel for treatment. The most reoccurring themes in the FGD were the need for more staff training about schistosomiasis, requests for diagnostic equipment, and the need to improve community response to schistosomiasis services in health facilities. Conclusions The prevalence of S. haematobium infection in patients seeking care in health facilities in Pemba is very low and similar to what has been reported from recent community-based cross-sectional surveys. The health facility staff had good schistosomiasis-related knowledge and practices. However, to integrate schistosomiasis patient management more durably into routine health facility activities, scalable screening pathways need to be identified and capacities need to be improved by regular staff training, and an unbroken supply of accurate point-of-care diagnostics and praziquantel for the treatment of cases. Graphical abstract

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“…The articles which were included are the ones which were original. The search of the articles and post-search analysis followed the statement given by the Preferred Reporting Items for Systematic Review and Meta-Analyses [5][6][7]. All available data which was generated from the two mentioned databases reporting cases of developing hyperkalaemia in east African countries were included.…”

Section: Search From the Internet And Criteria Used In Selectionmentioning

confidence: 99%

Occurrence and contributing factors associated with hyperkalaemia in East Africa: a systematic review

A.M,

I.V,

Odongo

et al. 2023

KJHS

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Hyperkalaemia is potentially a life-threatening medical condition characterized by higher-than-normal potassium in the blood. It can result in cardiac arrhythmias which is a serious heart problem and subsequently can lead to a sudden death. Impaired kidney function, such as chronic kidney disease or acute kidney injury, can hinder the proper excretion of potassium, leading to its accumulation in the blood. The prevalence of hyperkalemia in the United States is reported to be 1.55% for the normal population and 6.35% for kidney disease patients. Hyperkalaemia can be too problematic for people with kidney diseases. The prevalence of chronic kidney disease ranges between 2% and 7% in Uganda. The majority of the CKD patients in Uganda are presented late with advanced symptoms such as hyperkalemia. However, a study done by Dorsthorst has found out that it’s not only renal-impaired patients who are at the risk of becoming hyperkalemic after deviant consumption of potassium-rich foods, but the healthy humans with normally functioning kidneys are as well at risk. The study also alludes that the severity of hyperkalemia in patients with kidney disease or with normal kidney functionality does not differ. This review work was aimed at finding out the different reported cases of hyperkalemia in the Eastern region of Africa as well as their contributing factors. Articles used in this review were obtained from the internet using two search engines: Scopus and PubMed. Articles written in languages other than English and those which were review papers were excluded. The articles were screened and data were extracted. The results showed that hyperkalemia is caused by factors such as an overdose of potassium pills, chronic kidney disease, drug-to-drug interactions, and renal failure among other factors. Cases of hyperkalemia were reported in Uganda, Ethiopia, Tanzania, and Rwanda. Hyperkalaemia can be managed by responding to its specific cause. The overall prevalence of hyperkalemia has been reported to be approximately 1.1% in the general population (people with normal kidney function). From this review, it can be concluded that hyperkalemia occurs at high rates in people suffering from kidney disease. However, drug-drug interactions, increased intake of potassium, and tumor lysis are other causes.

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UCP-LF and other assay methods for schistosome circulating anodic antigen between 1978 and 2022

Cited by 7 publications

References 92 publications

Point-of-care potentials of lateral flow-based field screening for Mycoplasma bovis infections: a literature review

Point-of-care potentials of lateral flow-based field screening for Mycoplasma bovis infections: a literature review

Capacities and needs of health care facilities for schistosomiasis diagnosis and management in elimination settings

Occurrence and contributing factors associated with hyperkalaemia in East Africa: a systematic review

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