Ubiquitylome study highlights ubiquitination of primary metabolism related proteins in fruit response to postharvest pathogen infection

Cheng, Yulin; Xia, Xuehan; Lin, Yunlong; Cao, Haohao; Yao, Juanni; Li, Zhengguo

doi:10.1016/j.postharvbio.2020.111142

Cited by 9 publications

(4 citation statements)

References 48 publications

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“…Protein extraction for proteome, phosphoproteome, and ubiquitylome analyses was performed as described previously [ 45 ]. After grinding, samples of infected and uninfected petals were placed in lysis buffer containing 10 mM dithiothreitol, 1% protease inhibitor, 1% phosphatase inhibitor, and 50 μM PR-619 (a general inhibitor of deubiquitylating enzymes) for sonication.…”

Section: Methodsmentioning

confidence: 99%

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Li,

Yao,

Ming

et al. 2023

Horticulture Research

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As two of the most abundant post-translational modifications (PTMs), phosphorylation and ubiquitination play a significant role in modulating plant–pathogen interactions and increasing evidence indicates their crosstalk in plant immunity. Rose (Rosa sp.) is one of the most important ornamental plants and is seriously infected by Botrytis cinerea. Here, integrated proteomics analysis was performed to detect global proteome, phosphorylation, and ubiquitination changes in rose upon B. cinerea infection and investigate the possible phosphorylation and ubiquitination crosstalk. A total of 6165 proteins, 11774 phosphorylation and 10582 ubiquitination sites, and 77 phosphorylation and 13 ubiquitination motifs were identified. B. cinerea infection resulted in 169 up-regulated and 122 down-regulated proteins, 291 up-regulated and 404 down-regulated phosphorylation sites, and 250 up-regulated and 634 down-regulated ubiquitination sites. There were 12 up-regulated PR10 proteins and half of them also showed reduced ubiquitination. A lot of kinases probably involved in plant pattern-triggered immunity (PTI) signaling were up-regulated phosphoproteins. Noticeably, numerous kinases and ubiquitination-related proteins also showed a significant change in ubiquitination and phosphorylation, respectively. A cross-comparison of phosphoproteome and ubiquitylome indicated that both two PTMs of 104 proteins were dynamically regulated, and many putative PTI signaling components in plant plasma membrane were co-regulated. Moreover, five selected proteins including four PR10 proteins and a plasma membrane aquaporin were proven to be involved in rose resistance to B. cinerea. Our study provides insights into the molecular mechanisms underlying rose resistance to B. cinerea and also increases the database of phosphorylation and ubiquitination sites in plants.

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Section: Methodsmentioning

confidence: 99%

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Li,

Yao,

Ming

et al. 2023

Horticulture Research

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“…Metabolomic profiling of citrus fruit with enhancement of disease resistance by postharvest heat treatment (HT) showed that HT induced the accumulation of sugars [121]. There was a significant change (upregulated predominantly) in primary metabolites including sugars, organic acids, and amino acids in citrus fruit upon Pd infection [90]. These results indicate that primary metabolites are probably also involved in citrus fruit resistance against Pd infection by fueling resistance responses.…”

Section: Plant Metabolism In Citrus Fruit Resistancementioning

confidence: 97%

“…In addition to the two major facilitator superfamily transporters PdMfs1 and PdMfs2, a putative sucrose transporter PdSUT1 is involved in Pd pathogenicity, which is proven by the ∆PdSUT1 mutant showing reduced Pd virulence on citrus fruit compared with the wild type strain [88]. Since fungal sucrose transporter is a homologue to plant sucrose transporters [89], and sucrose is induced in citrus fruit upon Pd infection [90], we infer that PdSUT1 is involved in Pd pathogenicity possibly by transporting sucrose from host citrus fruit to promote Pd development.…”

Section: Transporters In Pd Pathogenicitymentioning

confidence: 99%

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

et al. 2020

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As the major postharvest disease of citrus fruit, postharvest green mold is caused by the necrotrophic fungus Penicillium digitatum (Pd), which leads to huge economic losses worldwide. Fungicides are still the main method currently used to control postharvest green mold in citrus fruit storage. Investigating molecular mechanisms of plant–pathogen interactions, including pathogenicity and plant resistance, is crucial for developing novel and safer strategies for effectively controlling plant diseases. Despite fruit–pathogen interactions remaining relatively unexplored compared with well-studied leaf–pathogen interactions, progress has occurred in the citrus fruit–Pd interaction in recent years, mainly due to their genome sequencing and establishment or optimization of their genetic transformation systems. Recent advances in Pd pathogenicity on citrus fruit and fruit resistance against Pd infection are summarized in this review.

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“…He et al [10] found that protein ubiquitination played a widely regulating role in rice seed germination by analyzing the ubiquitinome pro les. Cheng et al [11] highlighted that protein ubiquitination, especially the proteins related to primary metabolism, involved in responding to post-harvest pathogen infection of sweet orange fruit. Furthermore, several investigations reported that protein ubiquitination played crucial roles in fruit ripening and quality.…”

Section: Introductionmentioning

confidence: 99%

A multilevel investigation to reveal the regulatory mechanism of lignin accumulation in juice sac granulation of pomelo

LIU,

Chen,

et al. 2024

Preprint

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Granulation of juice sacs is a physiological disorder, which affects pomelo fruit quality. Here, the transcriptome and ubiquitinome of the granulated juice sacs were analyzed in Guanxi pomelo. We found that lignin accumulation in the granulated juice sacs was regulated at transcription and protein modification levels. In transcriptome data, we found that the genes in lignin biosynthesis pathway and antioxidant enzyme system of the granulated juice sacs were significantly up-regulated. However, in ubiquitinome data, we found that ubiquitinated antioxidant enzymes increased in abundance but the enzyme activities decreased after the modification, which gave rise to reactive oxygen species (ROS) contents in granulated juice sacs. This finding suggests that ubiquitination level of the antioxidant enzymes is negatively correlated with the enzyme activities. Increased H2O2 is considered to be a signalling molecule to activate the key gene expressions in lignin biosynthesis pathway, which leads to the lignification in granulated juice sacs of pomelo. This regulatory mechanism in juice sac granulation of pomelo was further confirmed through the verification experiment using tissue culture by adding H2O2 or dimethylthiourea (DMTU). Our findings suggest that scavenging H2O2 and other ROS are important for reducing lignin accumulation, alleviating juice sac granulation and improving pomelo fruit quality.

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Ubiquitylome study highlights ubiquitination of primary metabolism related proteins in fruit response to postharvest pathogen infection

Cited by 9 publications

References 48 publications

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

A multilevel investigation to reveal the regulatory mechanism of lignin accumulation in juice sac granulation of pomelo

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