Ubiquitin–proteasome system components are upregulated during intestinal regeneration

Pastén, Consuelo; Ortíz-Pineda, Pablo A.; García‐Arrarás, José E.

doi:10.1002/dvg.20803

Cited by 18 publications

(12 citation statements)

References 66 publications

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“…Our previous studies suggested that the proteasome could be playing an important role during intestinal regeneration in H. glaberrima (Pasten et al, 2012). Therefore, our first step was to determine if proteosomal enzymatic activity was present in extracts of regenerating intestine.…”

Section: Resultsmentioning

confidence: 99%

“…We had recently characterized proteasome components from H. glaberrima and shown them to be overexpressed during intestinal regeneration (Pasten et al, 2012). Thus our hypothesis was that, similar to what occurs in other systems (Glickman and Ciechanover, 2002), proteasomes could modulate different biological processes during intestinal regeneration, such as cell division or apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous report, we characterized several Ubiquitin Proteasome System (UPS) components of the sea cucumber, Holothuria glaberrima and showed that they displayed significant up-regulation during the first weeks of intestinal regeneration (Pasten et al, 2012). Moreover, we showed that when regenerating animals were treated with MG132, a proteasome inhibitor, the size of the regenerating rudiment was significantly reduced, suggesting a regulatory role of proteasomes during intestinal regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of proteolytic activities during intestinal regeneration of the sea cucumber, Holothuria glaberrima

Pastén¹,

Rosa²,

Ortiz³

et al. 2012

Int. J. Dev. Biol.

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Proteolysis carried out by different proteases control cellular processes during development and regeneration. Here we investigated the function of the proteasome and other proteases in the process of intestinal regeneration using as a model the sea cucumber Holothuria glaberrima. This echinoderm possesses the ability to regenerate its viscera after a process of evisceration. Enzymatic activity assays showed that intestinal extracts at different stages of regeneration possessed chymotrypsin-like activity. This activity was inhibited by i) MG132, a reversible inhibitor of chymotrypsin and peptidylglutamyl peptidase hydrolase (PGPH) activities of the proteasome, ii) E64d, a permeable inhibitor of cysteine proteases and iii) TPCK, a serine chymotrypsin inhibitor, but not by epoxomicin, an irreversible and potent inhibitor of all enzymatic activities of the proteasome. To elucidate the role which these proteases might play during intestinal regeneration, we carried out in vivo experiments injecting MG132, E64d and TPCK into regenerating animals. The results showed effects on the size of the regenerating intestine, cell proliferation and collagen degradation. These findings suggest that proteolysis by several proteases is important in the regulation of intestinal regeneration in H. glaberrima.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of proteolytic activities during intestinal regeneration of the sea cucumber, Holothuria glaberrima

Pastén¹,

Rosa²,

Ortiz³

et al. 2012

Int. J. Dev. Biol.

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“…In echinoderms, Patruno et al () demonstrated that levels of conjugated ubiquitin increased during the early post‐amputation phase and later growth during arm regeneration in the brittle star Amphiura filiformis , reflected in highly expressed transcripts for components of the protein degradation system (polyubiquitin and proteasome subunits) and structural proteins, such as actin and collagen (Burns et al ). Pasten et al () determined the expression levels of Rpn10, b3, and Ub‐RPL40 during early intestinal regeneration (3, 7, and 14 d) and compared them with those of normal intestines using gene expression microarrays in the sea cucumber, Holothuria glaberrima . Rpn10, β3, and Ub‐RPL40 genes were expressed at all stages of regeneration and in normal animals, but expression levels were significantly higher during the very early stages of intestinal regeneration.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the 26S Proteasome S7 Subunit Gene and Its Expression during Regeneration in Apostichopus japonicus

Qin

Zhao

et al. 2015

J World Aquaculture Soc

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The 26S proteasome, which includes 20S core and 19S regulatory particles, plays an important role in removing harmful and/or unnecessary proteins. In this study, we cloned a 2443‐bp full‐length cDNA sequence of the S7 subunit of 19S from the sea cucumber Apostichopus japonicus, including a 70‐bp 5′‐untranslated region (UTR) and 1070‐bp 3′‐UTR coding for a theoretical 435 amino acid protein. Sequence analysis showed that S7 belongs to the ATPases associated with a wide variety of cellular activities (AAA+) superfamily. S7 was widely expressed in different tissues as determined by real‐time quantitative polymerase chain reaction, and its expression was gradually upregulated in the regenerating body wall. Combined with histological observations, our results suggest that the S7 subunit or the 26S proteasome assembled based on S7 may be associated with the regulation of cell migration and cell cycle during body wall regeneration in A. japonicus.

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“…Recently high throughput gene expression techniques have been applied to echinoderm regeneration significantly increasing the number of candidate genes and timescales involved in this process. Gene expression profiling of regenerating ophiuroid arms and holothurians intestine revealed that some of the differentially expressed genes are homologs of genes known to be involved in regenerationrelated processes such as wound healing, cell proliferation, differentiation, morphological plasticity, cell survival, stress response, pathogenic challenge, and neoplastic transformation [126,[133][134][135][136][137][138][139].…”

Section: Echinodermsmentioning

confidence: 99%

Understanding regeneration through proteomics

et al. 2013

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Regeneration is a complex cellular process that, rather than simply forming a scar following injury, the animal forms a new functional tissue. Regeneration is a widespread process among metazoa, although not uniformly. Planaria, starfish, and some worms can regenerate most of their body, whereas many other species can only regenerate parts of specific tissues or fail to accomplish a functional regrowth, as is the case of mammals CNS. Research in regenerative medicine will possibly culminate in the regeneration of organs/tissues originally not prone to this process. Despite the complexity of the interactions and regulatory systems involved, the variety of tissues and organs these cells differentiate into has so far impaired the success of direct transplantation to restore damaged tissues. For this reason, a study, at the molecular level of the regeneration mechanisms developed by different animal models is likely to provide answers to why these processes are not readily activated in mammals. Proteomic-based approaches are being recognized as extremely useful to study of regeneration events, also because there is a relevant contribution of posttranscriptional processes that involve frequently the occurrence of a broad range of PTMs. The present review focuses on the significant knowledge brought up by proteomics in diverse aspects of regeneration research on different animal models, tissues, and organs.

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Ubiquitin–proteasome system components are upregulated during intestinal regeneration

Cited by 18 publications

References 66 publications

Characterization of proteolytic activities during intestinal regeneration of the sea cucumber, Holothuria glaberrima

Characterization of proteolytic activities during intestinal regeneration of the sea cucumber, Holothuria glaberrima

Characterization of the 26S Proteasome S7 Subunit Gene and Its Expression during Regeneration in Apostichopus japonicus

Understanding regeneration through proteomics

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