The results are reported of enzymatic-colorimetric determinations of citrate concentrations in aqueous humour and plasma, and of pyruvate concentrations in aqueous humour from cattle aged from 1 to 10 years. The mean citrate concentration in aqueous humour samples from 32 animals was 86.5 k 16.3 pmolil (mean value k standard deviation). I n plasma samples from 27 animals the mean citrate concentration was 110.3 k 47.2 pmolll. I n aqueous humour samples from 24 of the 32 cattles, the mean pyruvate concentration was 172.6 k35.6 pmolll. Regression analysis revealed a relationship between age and the citrate concentrations in aqueous humour (p < 0.001) and between age and the pyruvate concentrations in aqueous humour (0.05 > p > 0.02), the citrate concentrations rising and the pyruvate concentrations falling with increasing age. No relationship was demonstrated between the plasma citrate concentrations and age (0.50 > p > 0.40).Citrate and pyruvate are important intermediates in energy metabolism. Both metabolites occupy key positions, pyruvate being situated in the EmbdenMeyerhof pathway and citrate in the Krebs citric acid cycle. In glycolysis, pyruvate is converted into lactate. I n addition, pyruvate may be oxidized via the citric acid cycle.