Über Microsporidien aus dem Nervensystem von Fischen (Glugea lophii Doflein) und die Hypertrophie der befallenen Ganglienzellen

Weißenberg, Richard

doi:10.1007/bf02978989

Cited by 15 publications

(5 citation statements)

References 3 publications

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“…The location of the microsporidian infection in the Japanese anglerfish is extremely similar to that described from both European and American lophiids (Weissenberg 1911c, 1976, Jakowska 1964, Takvorian and Cali 1986; infections being found in the trigeminal nerves, spinal nerves of the vertebral column, vagal nerves near the kidney and on the medulla oblongata region of the hind brain. However, infection was never observed on the optic chiasma as described from the American anglerfish (Takvorian and Cali 1986).…”

Section: Discussionsupporting

confidence: 74%

“…However, infection was never observed on the optic chiasma as described from the American anglerfish (Takvorian and Cali 1986). Histological examination of infected tissues also revealed very similar infections in the nerve ganglia to those reported from other lophiid species, with the xenomas being restricted to the distal portion of vastly hypertrophied host nerve cells (Mrázek 1899, Weissenberg 1911c, 1976, Jakowska 1964. However, the distinct spore zonation patterns present in the xenomas from European anglerfish were never observed in the Japanese species and any heterogeneous nature to the xenomas was attributed to the developing stages of the uninucleate spores, often observed at the median layer in the xenoma, as described by Weissenberg (1976).…”

Section: Discussionsupporting

confidence: 60%

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A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

Freeman

Yokoyama²,

Ogawa³

2004

FOLIA PARASIT

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Abstract. In the present study, a high percentage of Japanese anglerfish, Lophius litulon (Jordan, 1902), contained a microsporidian infection of the nervous tissues. Xenomas were removed and prepared for standard wax histology and transmission electron microscopy (TEM). DNA extractions were performed on parasite spores and used in PCR and sequencing reactions. Fresh spores measured 3.4 × 1.8 µm and were uniform in size with no dimorphism observed. TEM confirmed that only a single developmental cycle and a single spore form were present. Small subunit (SSU) rDNA sequences were >99.5% similar to those of Spraguea lophii (Doflein, 1898) and Glugea americanus (Takvorian et Cali, 1986) from the European and American Lophius spp. respectively. The microsporidian from the nervous tissue of L. litulon undoubtedly belongs in the genus Spraguea Sprague et Vávra, 1976 and the authors suggest a revision to the generic description of Spraguea to include monomorphic forms and the transfer of Glugea americanus to Spraguea americana comb. n. Since no major differences in ultrastructure or SSU rDNA sequence data exist between Spraguea americana and the microsporidian from the Japanese anglerfish, they evidently belong to the same species. This report of Spraguea americana is the first report of a Spraguea species from L. litulon and indeed from the Pacific water mass.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionsupporting

confidence: 60%

A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

Freeman

Yokoyama²,

Ogawa³

2004

FOLIA PARASIT

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“…In the hypertrophy of glomerular cells in the kidney of Esox lucius invaded by developmental stages of a myxospridan, the host cell remains uninucleate(23,25). The same is true in the hypertrophying ganglion cells of Lophius infected by the microspwridan Nosema lop& ( 7,17). In the lymphmystis virus xenomas of fibroblasts in perches and flatfishes, 2 nuclei are sometimes observed in the host cell, but the grraat majority of host cells m a i n uninucleate( 20,21,23,27).…”

Section: Discussionmentioning

confidence: 90%

Intracellular Development of the Microsporidan Glugea anomala Moniez in Hypertrophying Migratory Cells of the Fish Gasterosteus aculeatus L., an Example of the Formation of “Xenoma” Tumors

Weißenberg¹

1968

The Journal of Protozoology

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SYNOPSIS. The conclusion drawn in 1921 that the large nuclei in the cytoplasmic cortex of Glugea cysts are not vegetative nuclei of the microsporidan but nuclei of the hypertrophied host cell was based on the discovery of early developmental stages in the mesenchyme of stickleback larvae experimentally fed Glugea spores. This observation had been made on serial sections from experiments done in 1912. The intracellular development of the microsporidan could be followed up in this material only thru the 1st stages of schizogony. Renewed infection experiments, done still in 1921 on a much broader basis, have fully confirmed the previous findings, as briefly stated in 1922. On this material, the intracellular development of G. anomala has been followed up in recent years from uninucleate host cells 7 μ in diameter, interpreted as wandering cells in the mesenchyme, until they became macroscopic multinucleate cysts, in which schizogony and sporogony of the microsporidan produced innumerable vegetative stages and spores of Glugea. The details of the developmental processes are described in the present paper. The multinucleate host cell and the intracellular parasites together form one of the symbiotic complexes for which the term “xenom” or “xenoma” has been used by me since 1949. By a sequence of amitotic nuclear divisions, the uninucleate host cell in the Glugea xenomas of Gasterosteus becomes plurinucleate in contrast to the usual structure of other xenomas of fish. Already in 1921, I thought that the host cell in the Glugea xenomas may have phagocytic properties. The observation of accumulation of granules from pigment cells in some of the Glugea xenomas has now verified this supposition.

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“…Among the 18 genera infecting fish, 12 of these produce xenomas: Amazonspora Azevedo and Matos 2003;Glugea Thélohan, 1891;Ichthyosporidium Caullery and Mesnil, 1905;Loma Morrison and Sprague, 1981;Microfilum Faye, Toguebaye and Bouix, 1991;Microgemma Ralphs and Matthews, 1986;Myosporidium Baquero, Rubio, Moura, Pieniazek and Jordana, 2005;Neonosemoides Faye, Toguebaye and Bouix, 1996;Pseudoloma Matthews, Brown, Larison, Bishop-Stewart, Rogers and Kent, 2001;Potaspora Casal, Matos, Teles-Grilo and Azevedo 2008;Spraguea Weissenberg 1976 andTetramicra Matthews andMatthews 1980. Among these microsporidians, the genus Spraguea is a typical case of close relationship between the kind of parasite, host and the place of infection. Presently, it is known that five (L. piscatorius, L. budegassa, L. americanus, L. litulon and L. gastrophysus) of seven lophiid species are parasitized in the nervous tissues (spinal nerves of the vertebral column, trigeminal nerves, vagal nerves or in the medulla oblongata region of the hind brain) by microsporidia belonging to the genus Spraguea (Jakowska 1964;Takvorian and Cali 1986;Weissenberg 1911cWeissenberg , 1976. One exception for this was observed in the anglerfish Lophius budegassa in Spain for the reason that the microsporidians Tetramicra brevifilum and Spraguea lophii were simultaneously found in hepatocytes and musculature, respectively (Maíllo et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Redefining the genus Spraguea based on ultrastructural and phylogenetic data from Spraguea gastrophysus n. sp. (Phylum Microsporidia), a parasite found in Lophius gastrophysus (Teleostei) from Brazil

et al. 2012

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The ultrastructure of the fish-infecting microsporidium Spraguea gastrophysus found in the dorsal ganglia and kidney of the anglerfish, Lophius gastrophysus (family Lophiidae) collected on the Brazilian Atlantic coast is described. Each whitish xenoma (up to 3.1 × 1.8 mm) contains several groups of parasites. The host cells are hypertrophied and contain various parasite life stages including mature spores and several developmental stages with unpaired nuclei. Monomorphic spores are ellipsoidal, lightly curved and measure about 3.35 × 1.71 μm. The spore contains a gradually tapering isofilar polar filament with five to six coils arranged in a single row. The nucleus occupies a central zone of the sporoplasm where also several polyribosomes are presented. The posterior vacuole contains a voluminous spherical and granular posterosome measuring up to ~0.65 μm in diameter. The partial small subunit, intergenic spacer and partial large subunit rRNA gene were sequenced and the phylogenetic analysis places the microsporidian described here in the clade that includes all sequences of the Spraguea genus. The ultrastructural morphology of the xenoma and the spores of this microsporidian parasite, as well as the molecular and phylogenetic analysis, suggest the description of a new species. A redefining of the genus Spraguea is also done.

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Über Microsporidien aus dem Nervensystem von Fischen (Glugea lophii Doflein) und die Hypertrophie der befallenen Ganglienzellen

Cited by 15 publications

References 3 publications

A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

A microsporidian parasite of the genus Spraguea in the nervous tissues of the Japanese anglerfish Lophius litulon

Intracellular Development of the Microsporidan Glugea anomala Moniez in Hypertrophying Migratory Cells of the Fish Gasterosteus aculeatus L., an Example of the Formation of “Xenoma” Tumors

Redefining the genus Spraguea based on ultrastructural and phylogenetic data from Spraguea gastrophysus n. sp. (Phylum Microsporidia), a parasite found in Lophius gastrophysus (Teleostei) from Brazil

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