Über Induktion von Medullarplatte durch Medullarplatte im Jüngeren Keim, ein Beispiel homöogenetischer oder assimilatorischer Induktion

Mangold, O.; Spemann, Hans

doi:10.1007/bf02080953

Cited by 138 publications

(23 citation statements)

References 10 publications

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“…We therefore made an anterior neuroectoderm (ANE) cDNA library from Xenopus late gastrulae to increase cloning efficiency, and screened it for genes that are involved in neuralization and neural patterning. We focused on the ANE to clarify the following features of ANE: (1) it might contain a source of secreted factors responsible for 'homeogenetic induction', neural induction by the neural plate (Mangold and Spemann, 1927;Servetnick and Grainger, 1991); (2) the ANE should have its own neuralizing activity as a default state after suppressing BMP signaling; and (3) the ANE may have self-regionalizing activity to give rise to brain structures from the forebrain to midbrain, and thus generate a wide variety of cell populations during neurulation.…”

Section: Introductionmentioning

confidence: 99%

XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 inXenopusembryos

2003

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A family of inner nuclear membrane proteins is implicated in gene regulation by interacting with chromatin, nuclear lamina and intranuclear proteins; however, the physiological functions of these proteins are largely unknown. Using a Xenopus expression screening approach with an anterior neuroectoderm cDNA library, we have identified an inner nuclear membrane protein, XMAN1, as a novel neuralizing factor that is encoded by theXenopus ortholog of human MAN1. XMAN1 mRNA is expressed maternally, and appears to be restricted to the entire ectoderm at the early gastrula stage, then to the anterior neuroectoderm at the neurula stage. XMAN1 induces anterior neural markers without mesoderm induction in ectodermal explants, and a partial secondary axis when expressed ventrally by dorsalizing the ventral mesoderm. Importantly, XMAN1 antagonizes bone morphogenetic protein (BMP) signaling downstream of its receptor Alk3, as judged by animal cap assays, in which XMAN1 blocks expression of downstream targets of BMP signaling (Xhox3 and Msx1), and by luciferase reporter assays, in which XMAN1 suppresses BMP-dependent activation of theXvent2 promoter. Deletion mutant analyses reveal that the neuralizing and BMP-antagonizing activities of XMAN1 reside in the C-terminal region, and that the C-terminal region binds to Smad1, Smad5 and Smad8, which are intracellular mediators of the BMP pathway. Interference with endogenous XMAN1 functions with antisense morpholino oligos leads to the reduction of anterior neuroectoderm. These results provide the first evidence that the nuclear envelope protein XMAN1 acts as a Smad-interacting protein to antagonize BMP signaling during Xenopus embryogenesis.

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Section: Introductionmentioning

confidence: 99%

XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 inXenopusembryos

2003

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“…In the development of a wide variety of animal tissues, a small number of pre-specified cells induce their neighbors to generate secondary cells with similar fates (Mangold and Spemann, 1927). This two-step induction strategy, termed homeogenetic induction, has the potential to produce an endless cascade of induction, leading to either malignant transformations or hyperproliferative diseases (reviewed by Tang et al, 1997;Zwick et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

EDL/MAE regulates EGF-mediated induction by antagonizing Ets transcription factor Pointed

2003

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“…Alternatively, the epithelial signals may induce the mesenchymal cells to express the same or other signalling molecules, which subsequently induce syndecan expression in mesenchymal cells. In this case the induction would be mediated via homoiogenetic mechanism (Mangold and Spemann, 1927;Kurihara and Sasaki, 1981;Ruis i Altaba, 1990;Servetnick and Grainger, 1991). The possibility that syndecan, which is expressed early on in the dental epithelium, would act as a signal molecule inducing its own expression has been mostly ruled out (Vainio et al, 1989a).…”

Section: Discussionmentioning

confidence: 99%

Coordinated induction of cell proliferation and syndecan expression in dental mesenchyme by epithelium: Evidence for diffusible signals

Vainio

1992

Developmental Dynamics

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Epithelial-mesenchymal interactions induce the expression of syndecan, a cell surface proteoglycan, and tenascin, an extracellular matrix glycoprotein in the mesenchymal component of many organ rudiments including the tooth. Experimental recombination cultures of early dental epithelium and mesenchyme were analysed by double immunostaining to compare the distribution of syndecan, tenascin, and proliferating cells (BrdU incorporation) in the induced dental mesenchyme. After 5-9 hr in culture expression of syndecan and tenascin as well as an increase in BrdU incorporation were evident in the mesenchymal cells adjacent to the epithelium and the positive area enlarged with time. Syndecan and tenascin were colocalized only partially in some explants. The expression of syndecan and tenascin in the recombinants correlates with their stage-dependent expression pattern during early tooth development in vivo (Vainio and Thesleff, 1992). The area of increased cell proliferation in the mesenchyme correlated closely with syndecan expression. In none of the explants was increased BrdU incorporation observed in syndecan negative areas. Epithelium induced also condensation of the mesenchymal cells. Induction and spread of the syndecan-positive zone in the dental mesenchyme required close and continuous contact with the epithelium. The mechanism by which the induction of syndecan expression spreads in the mesenchyme was studied in rat-mouse interspecies recombination cultures, using syndecan antibodies that recognize mouse but not rat syndecan. The rat mesenchyme and epithelium were first cultured in contact for 24 hr. Then the epithelium was removed and freshly dissected, "uninduced" mouse mesenchyme was placed in contact with different aspects of the rat mesenchyme. The rat mesenchymal cells that had located next to the epithelial tissue stimulated syndecan expression in adjacent mouse mesenchyme. The induction potential was gradually lost toward the periphery of the rat mesenchyme. Based on these findings we suggest that diffusible signal molecules mediate the spread of syndecan induction in the mesenchyme and that syndecan plays a role in the regulation of cell proliferation.

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Über Induktion von Medullarplatte durch Medullarplatte im Jüngeren Keim, ein Beispiel homöogenetischer oder assimilatorischer Induktion

Cited by 138 publications

References 10 publications

XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 inXenopusembryos

XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 inXenopusembryos

EDL/MAE regulates EGF-mediated induction by antagonizing Ets transcription factor Pointed

Coordinated induction of cell proliferation and syndecan expression in dental mesenchyme by epithelium: Evidence for diffusible signals

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