Über die ozonisierungsreaktion von jod in wasserfreiem fluorwasserstoff und die hydrolyse von jodtrifluorid

Synthesis of the N-Terminal N-Myristoylated and S-Palmitoylated Undetrigintapeptide of Endothelial NO-Synthase

“…[4,5] The localization of eNOS to the plasma membrane and its concentration in the caveolae, membrane microdomains highly enriched in various signal transducing proteins is crucial to its correct biological functioning. [6] In contrast to the other isoforms of NO-synthase identified so far, the N-terminus of eNOS is N-myristoylated and twice S-palmitoylated (see Figure 1 and 1, Scheme 1). [7] The lipid until the IF 3 dissolved largely or totally on shaking and the HF solution was a yellow-brown color.…”

“…19 F NMR (n-C 6 groups are required for plasma membrane localization and biological activity. [6,8] However, the precise biological roles fulfilled by the lipidated part of the protein are subject to various hypotheses.…”

“…[6,8] However, the precise biological roles fulfilled by the lipidated part of the protein are subject to various hypotheses. In particular, notions have been forwarded that the lipid groups might be responsible for selective targeting of eNOS at the plasma membrane and caveolae, for example, by mediatiation of protein/protein or protein/lipid interactions, and that palmitoylation/depalmitoylation might be involved in signalling through eNOS.…”

“…[4] A second drawback is the short wavelengths for the excitation maxima of the fluorophores. Furthermore, oxidation of the known fluorogenic substrates yields, in most cases, a mixture of reaction products rather than one well defined fluorophore [6] and, in some cases, not even the exact structure of the fluorescent compounds obtained could be elucidated.…”

Die Struktur von IF3

“…[5] Der gelbe Feststoff ist in allen gängigen Lösungsmitteln entweder unlöslich oder er reagiert mit ihnen, so dass Strukturinformationen bisher nur mit der Schwingungsspektroskopie erhalten werden konnten. [6] Es war schon bekannt, dass sich IF 3 spurenweise in wasserfreiem HF löst, wie an einer leichten Gelbfärbung der Lösung zu erkennen ist. Die Zugabe von H 2 O in kleinen Mengen erhöht die Löslichkeit, wobei die Lösung eine tiefgelbe Farbe mit einem Stich ins Bräunliche annimmt.…”