“…In rats it has been shown to lead to the production of fatty livers, followed after some time by cirrhosis. More recent work has shown that this may be followed eventually by the development of hepatoma-like tumours (Copeland and Salmon, 1946;Staub, Viollier and Werthemann, 1948). Furthermore, choline deficiency of a severity not sufficient to produce cirrhosis predisposes the liver to the carcinogenic action of Butter Yellow (Opie, 1944;Buckley, Buckley and Snipes, 1951).…”