UBE2C is a Potential Biomarker for Tumorigenesis and Prognosis in Tongue Squamous Cell Carcinoma

Liu, Pei‐Feng; Chen, Chunfeng; Shu, Chih‐Wen; Chang, Hyo Sik; Lee, Cheng-Hsin; Liou, Horng-Huei; Ger, Luo‐Ping; Chen, Chunlin; Kang, Bor‐Hwang

doi:10.3390/diagnostics10090674

Cited by 24 publications

(23 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these, UBE2C has been demonstrated to be a high tumor marker associated with advanced cancer. In malignant glioma, breast cancer, gastric cancer, melanoma, endometrial cancer, head and neck squamous cell carcinoma, and oral squamous cell carcinoma (OSCC), UBE2C is associated with poor prognosis [43][44][45][46][47][48][49][50]. In EMT-related genes, Claudin-7 (CLDN7) promotes EMT in colon cancer and ovarian cancer enhanced invasion [51,52].…”

Section: Discussionmentioning

confidence: 99%

Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma

Luo

Liu

Chang

et al. 2021

IJMS

View full text Add to dashboard Cite

Ribosome-binding protein 1 (RRBP1) is a potential oncogene in several cancer types. However, the correlation between RRBP1 expression and the prognosis of patients with upper tract urothelial carcinoma (UTUC) remains unclear. In this study, we identified that RRBP1 is associated with carcinogenesis and metastasis in UTUC using a methylation profiling microarray. High correlations between RRBP1 and cancer stages, nodal metastasis status, molecular subtypes, and prognosis in bladder urothelial cancer (BLCA) were found. Aberrant DNA methylation in the gene body region of RRBP1 was determined in UTUC tissues by methylation-specific PCR. RRBP1 expression was significantly increased in UTUC tissues and cell lines, as determined by real-time PCR and immunohistochemistry. RRBP1 depletion significantly reduced BFTC909 cell growth induced by specific shRNA. On the other hand, molecular subtype analysis showed that the expression of RRBP1 was associated with genes related to cell proliferation, epithelial–mesenchymal transition, and basal markers. A patient-derived organoid model was established to analyze patients’ responses to different drugs. The expression of RRBP1 was related to chemoresistance. Taken together, these results provide the first evidence that RRBP1 gene body hypomethylation predicts RRBP1 high expression in UTUC. The data highlight the importance of RRBP1 in UTUC malignancy and chemotherapeutic tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma

Luo

Liu

Chang

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…About 5-7 × 10 5 cells/mL cultured in 96-well plates were lysed by adding 100 µL of CellTiter-Glo reagent from the CellTiter-Glo luminescent cell viability assay kit (Promega, Madison, WI, USA) for 10 min and cell viability was measured by luminescence signal in a luminometer [38].…”

Section: Cell Viabilitymentioning

confidence: 99%

“…After invasion, cells adhered to the underside of the filter were fixed in 4% formaldehyde and stained with 0.1% crystal violet. The number of migrated cells was observed and quantified [38].…”

Section: Cell Invasion Assaymentioning

confidence: 99%

“…The IBIDI Culture-Inserts (IBIDI Culture-Inserts (IBIDI, Inc., Planegg, Germany) were first attached to the culture plates, then cells (in 140 µL DMEM at a density of 1.5 × 10 5 cells/mL) were cultured in the insert overnight. Subsequently, the insert was removed, and cells were rinsed with PBS and cultured in DMEM to observe the distance between migrated cells [38].…”

Section: Wound-healing Assaymentioning

confidence: 99%

See 1 more Smart Citation

Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma

Tseng

Chen

Shu

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Oral squamous cell carcinoma (OSCC) is one of the most common types of malignant tumor. Sequestosome 1 (SQSTM1) serves as an adaptor of autophagy for degrading protein aggregates. The regulation of autophagy by EGFR and its clinical impacts are indicated in various types of cancer. However, the association of EGFR and SQSTM1 in OSCC is still unknown. Our results show that the expression levels of SQSTM1 and EGFR proteins are higher in tumor tissues than in the corresponding tumor-adjacent (CTAN) tissues of OSCC patients. The expression levels of SQSTM1 were positively associated with the EGFR expression level. High co-expression of SQSTM1 and EGFR is associated with poor prognosis in OSCC patients. Moreover, SQSTM1 expression is decreased in EGFR-knockdown cells. Cell growth and invasion/migration are also decreased in cells with single/combined knockdowns of EGFR and SQSTM1 or in SQSTM1-knockdown cells without EGFR kinase inhibitor Lapatinib treatment compared to that in scrambled cells. However, cell growth and invasion/metastasis were not significantly different between the scrambled cells and SQSTM1-knockdown cells in the presence of Lapatinib. This study is the first to indicate the biological roles and clinical significance of SQSTM1 regulation by EGFR in OSCC.

show abstract

“…For cell migration, the cells (2 × 10 5 cells) were cultured into the IBIDI Culture-Inserts (IBIDI, Inc., Planegg, Germany) for 24 h within the culture dishes. Subsequently, the plastic inserts were removed, wound healing was observed, and the migration distance was quantified [20].…”

Section: Cell Invasion and Migrationmentioning

confidence: 99%

Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Liu

Shu

Lee

et al. 2021

Cancers

Self Cite

View full text Add to dashboard Cite

Guanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. However, the role played by GBP5 in cancer development, especially in oral squamous cell carcinoma (OSCC), is still unknown. Herein, next-generation sequencing analysis showed that the gene expression levels of GBP5 were significantly higher in OSCC tissues compared with those found in corresponding tumor adjacent normal tissues (CTAN) from two pairs of OSCC patients. Higher gene expression levels of GBP5 were also found in tumor tissues of 23 buccal mucosal squamous cell carcinoma (BMSCC)/14 tongue squamous cell carcinoma (TSCC) patients and 30 oral cancer patients from The Cancer Genome Atlas (TCGA) database compared with those in CTAN tissues. Immunohistochemical results showed that protein expression levels of GBP5 were also higher in the tumor tissues of 353 OSCC patients including 117 BMSCC, 187 TSCC, and 49 lip squamous cell carcinoma patients. Moreover, TCGA database analysis indicated that high gene expression levels of GBP5 were associated with poor overall survival in oral cancer patients with moderate/poor cell differentiation, and associated with poor disease-free survival in oral cancer patients with moderate/poor cell differentiation and lymph node metastasis. Furthermore, GBP5-knockdowned cells exhibited decreased cell growth, arrest at G1 phase, and decreased invasion/migration. The gene expression of markers for epithelial−mesenchymal transition and cancer stemness was also reduced in GBP5-silenced oral cancer cells. Taken together, GBP5 might be a potential biomarker and therapeutic target for OSCC patients, especially for those with poor cell differentiation and lymph node metastasis.

show abstract

UBE2C is a Potential Biomarker for Tumorigenesis and Prognosis in Tongue Squamous Cell Carcinoma

Cited by 24 publications

References 40 publications

Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma

Hypomethylated RRBP1 Potentiates Tumor Malignancy and Chemoresistance in Upper Tract Urothelial Carcinoma

Effect of EGFR on SQSTM1 Expression in Malignancy and Tumor Progression of Oral Squamous Cell Carcinoma

Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma

Contact Info

Product

Resources

About