UbcH10 expression on thyroid fine‐needle aspirates

Abstract

BACKGROUND:Thyroid fine‐needle aspiration (FNA) samples belonging to the follicular neoplasm/suspicious for malignancy classes are controversial. The authors identified UbcH10 as a marker useful in the diagnosis of several neoplasms, including thyroid cancer. Here, analysis of UbcH10 expression by quantitative reverse transcriptase polymerase chain reaction (RT‐PCR) and immunohistochemistry was applied to FNAs.METHODS:A series of 84 follicular neoplasm/suspicious for malignancy FNAs with histological follow‐up (30 malignant) was prospectively collected. UbcH10 immunostaining was performed on cell blocks and compared with that of the proliferation marker Ki‐67. At the mRNA level, UbcH10 was compared with CCND2 and PCSK2 expression, these latter being the best performing components of the previously reported 3‐gene assay; to determine the diagnostic accuracy, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve for each gene individually and in combination was evaluated.RESULTS:UbcH10 and Ki‐67 shared a similar pattern; although UbcH10 expression was higher in malignant than in benign lesions (P < .001), staining was sporadic, and the cutoff value derived by the ROC analysis was too low (1.25%) for routine application. Conversely, UbcH10 expression assessment by quantitative RT‐PCR was effective. UbcH10 mRNA levels associated with malignant histology were significantly higher than those associated with benign histology (P = .02). The AUC was 0.74 for UbcH10, 0.81 for CCDN2, 0.62 for PCSK2, and 0.84 for UbcH10 and CCND2 combination.CONCLUSIONS:UbcH10 quantitative RT‐PCR analysis, rather than immunohistochemistry, is useful to increase the detection of malignancy in thyroid FNAs. UbcH10 may be added as a panel component in quantitative RT‐PCR–based assays. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society.

Molecular Assays in Cytopathology for Thyroid Cancer

“…45,46 However, neither has demonstrated sufficiently high sensitivity or specificity to justify widespread use. Nonetheless, their combined use, along with other markers, continues to be explored.…”

Dominant negative Ubiquitin-conjugating enzyme E2C sensitizes cervical cancer cells to radiation

“…Overexpression of UBE2C has been demonstrated in a variety of malignancies such as colorectal, thyroid, lung, breast, ovary and lymphomas corresponding to their normal tissues (Warrenfeltz et al 630 M. V. Bose et al . 2004, Kadara et al 2009, Loussouarn et al 2009, Troncone et al 2009, Chen et al 2010, Guerriero et al 2010.…”

Molecular diagnosis for indeterminate thyroid nodules on fine needle aspiration: advances and limitations

“…All samples were indeterminate lesions with a cytopathological diagnosis of follicular neoplasm or suspicious for malignancy (Bethesda IV and V). Sensitivity and specificity for classifying indeterminate nodules remained fairly low at 72 and 67%, respectively [55]. The study's authors suggested that UbcH10 could, however, be added as a useful adjunct to other gene panels.…”

“…UBE2C (or UbcH10) is associated with destruction of cell cycle cyclins and facilitation of cell cycle progression [55]. One study by Guerriero et al examined UbcH10 expression using RT-PCR and immunohistochemistry in 84 FNA samples.…”