UBASH3B Is a Novel Prognostic Biomarker and Correlated With Immune Infiltrates in Prostate Cancer

Abstract

Background: UBASH3B (STS1) is an important gene that negatively regulates T-cell receptor signaling in activated T-lymphocytes that involved in cancers. However, the function of UBASH3B in prostate cancer (PCa) and the correlation between UBASH3B and tumor-infiltrating immune cells still remain unclear. Methods: Real-time PCR and immunohistochemistry were applied to detect mRNA and protein expression of UBASH3B in PCa patients and benign prostate hyperplasia patients (BPH). Clinical features of patients with PCa were recorded and Kaplan Meier curve was subsequently plotted. Based on mRNA expression of UBASH3B, patients with PCa from TCGA database were divided into low-UBASH3B-expression group and high-UBASH3B-expression group for construct lncRNA-miRNA-mRNA network and analyzing GO and KEGG pathways. Single gene analysis method was performed by using GSEA to interpret gene expression data in PCa. The PPI network was constructed using STRING and the correlation between UBASH3B and tumor-infiltrating immune cells was analyzed by TIMER and CIBERSORT. Results: The mRNA and protein expression of UBASH3B were upregulated in PCa. The abundant expression of UBASH3B is associated with poor prognosis in PCa. The subnetwork of UBASH3B contains three lncRNAs (MIAT, LINC01297, MYLK-AS1) and four miRNAs (hsa-miR-200a-3p, hsa-miR-455-5p, hsa-miR-192-5p, hsamiR-215-5P). The mRNA expression of UBASH3B was involved in 28 KEGG pathways. GSEA analysis showed that 18 hallmark gene sets were significantly enriched in high-UBASH3B-expression, whereas 1 gene set was enriched in low-UBASH3B-expression. PPI network revealed a tightly interaction between UBASH3B and LCP2 (an immune related gene). TIMER and CIBERSORT database indicated that UBASH3B was correlated with 11 types of tumor-infiltrating immune cells (naïve B cell, memory B cells, resting CD4 + memory T cell, activated CD4 + memory T cell, regulatory T cell, activated NK cell, M2 macrophages, resting dendritic cells, activated dendritic cells, resting mast cells, neutrophils). Conclusions: In conclusion, UBASH3B may be a novel potential prognostic biomarker and is associated with tumor-infiltrating immune cells in tumor microenvironment, suggesting UBASH3B as a potential target for future treatment of PCa.

Potential role of m6A RNA methylation regulators in osteosarcoma and its clinical prognostic value

“…Timer was used to analyze the tumor-infiltrating immune cells and the correlations between the infiltrating level of different subsets of immune cells and IGF2BP2 high and low expression samples. Cibersort was used for estimating the abundance of different immune cell types in tumor microenvironment by FPKM data [ 26 ].…”

Emerging Role of MiR-192-5p in Human Diseases

“…Fortunately, a recent study by Chen and coworkers reported that the expression of miR-192-5p is aberrantly increased in prostate cancer tissues, and the enhanced expression of miR-192-5p promotes the proliferation of prostate cancer cells in vitro ( Chen ZJ et al, 2019 ). Additionally, bioinformatics analyses have revealed that miR-192-5p is associated with ubiquitin-associated and SH3 domain-containing B (UBASH3B), a poor prognostic marker of prostate cancer ( Wang et al, 2019 ). These two studies reveal the possible regulatory role of miR-192-5p in prostate cancer.…”

Early development and functional properties of tryptase/chymase double-positive mast cells from human pluripotent stem cells

“…Compared with the other clusters, the MP cluster exhibited MC-like development features, such as high expression of STAT5A/STAT5B , SRGN , MAPK14 , and MITF ( Shelburne et al, 2003 ; Abrink et al, 2004 ; Morii et al, 2004 ; Hu et al, 2012 ). Furthermore, several genes related to MC activation, including FCER1G , UNC13D , CD84 , CD226 , LCP2 , STXBP2 , and BTK , were highly expressed in this cluster ( Figure 3B; Bachelet et al, 2006 ; Álvarez-Errico et al, 2011 ; Oksaharju et al, 2011 ; Bin et al, 2013 ; Rodarte et al, 2018 ; Simonowski et al, 2020 ; Wang et al, 2020 ). However, in this cluster, the endothelial markers PECAM1 and ITGB3 were highly expressed, while the hematopoietic markers SPN and PTPRC were weakly expressed and absent, respectively ( Supplementary Figure S3D ).…”